ABSTRACT
BACKGROUND/METHODS: Transmission of 362 nm ultraviolet A radiation (UVA) through epithelium of oral mucosa and skin epidermis was compared in rat and man. The transmitted irradiance was measured by means of a radiometer. RESULTS: In man, significantly (P<0.01) greater transmittance (2.8-fold) was observed in skin compared to buccal mucosa. In rat, no significant differences were found. CONCLUSION: In different species and different types of epitheliae, separate studies are needed for the evaluation of UVA transmission.
Subject(s)
Epidermis/radiation effects , Mouth Mucosa/radiation effects , Ultraviolet Rays , Animals , Humans , Optics and Photonics , RatsABSTRACT
BACKGROUND/PURPOSE: Ultraviolet-A radiation (UVA) of the oral mucosa after photosensitization with either systemic methoxsalen (8-MOP) or topical trioxsalen (TMP), i.e. mouth-PUVA, has been reported to be successful in the treatment of oral lichenoid lesions. In the case of PUVA treatment of skin disorders, local immune suppressive effects have been demonstrated, and the antigen presenting epithelial Langerhans cells (LCs) have been shown to be especially sensitive to ultraviolet treatments. Our aim was to compare the photobiological effects of PUVA in oral mucous membrane (OMM) using topical TMP or systemic 8-MOP photosensitization. METHODS: Rat OMM photosensitized with topical TMP or systemic 8-MOP was treated with PUVA using UVA doses of 1-8 J/cm2. The LCs were demonstrated in epithelial sheets of the treated OMM with ATPase staining. RESULTS: Both treatments caused a sim ilar, dose-dependent depletion of ATPase-positive LCs, with a maximal depletion of 80% or 73% with 8 J/cm2 at 2 days after irradiation as photosensitized with TMP or 8-MOP, respectively. This contrasts with earlier published findings in human skin, where topical TMP is an order of magnitude greater a sensitizer than 8-MOP, and PUVA-induced depletion of LCs occurs maximally 5 days after irradiation. CONCLUSION: The depletion of LCs of rat OMM after PUVA treatment is greater using topical TMP compared to systemic 8-MOP, but the difference is significantly smaller than reported earlier in human skin.
Subject(s)
Langerhans Cells/drug effects , Methoxsalen/pharmacology , Mouth Mucosa/drug effects , PUVA Therapy , Photosensitizing Agents/pharmacology , Trioxsalen/pharmacology , Administration, Oral , Administration, Topical , Animals , Dose-Response Relationship, Drug , Epithelium/drug effects , Male , Rats , Rats, Long-EvansABSTRACT
OBJECTIVE: To investigate the feasibility of topical psoralen PUVA (sensitization in photosensitizing psoralen drug + UVA radiation) treatment of oral lichenoid lesions (OLL). STUDY DESIGN: A total of 16 patients with OLL were treated using a 0.01% trioxsalen ointment and UVA doses in the 0.09 to 1.80 J/cm2 range. The average number of sessions was 8.7 and a mean cumulative irradiation dose was 4.25 J/cm2. RESULTS: A marked-to-complete healing occurred in 3 to 16 (19%) patients immediately after therapy, in 4 of 14 (29%) after 3 months, and in 5 of 14 (38%) after 14 months, respectively. Of the 16 subjects with OLL, five were diagnosed as oral lichen planus (OLP) and 11 were classified as oral lichenoid reaction (OLR). Post-PUVA amelioration rate in patients with genuine OLP (4 of 5, 80%) was superior to that in patients with OLR (1 of 9, 11%). CONCLUSION: Topical trioxsalen photosensitization can be used in mouth-PUVA treatment, and lichen planus is a main indication for this therapy.
Subject(s)
Lichen Planus, Oral/drug therapy , PUVA Therapy/methods , Administration, Topical , Adolescent , Adult , Aged , Feasibility Studies , Female , Follow-Up Studies , Humans , Lichenoid Eruptions/drug therapy , Male , Middle Aged , Mouth Diseases/drug therapy , Ointments , Photosensitizing Agents/administration & dosage , Photosensitizing Agents/therapeutic use , Radiation Dosage , Remission Induction , Treatment Outcome , Trioxsalen/administration & dosage , Trioxsalen/therapeutic useABSTRACT
Four holes drilled in rabbit tibia were filled with different commercial hydroxyapatite products. All particles caused a mild inflammatory reaction, which disappeared in 2 months. The line between the new and original bone was visible even after 6 months. No differences in healing pattern were discovered for the various products. When bone cavities are filled with hydroxyapatite particles, the shape of the particles does not seem to affect the healing process.
Subject(s)
Bone and Bones/physiopathology , Hydroxyapatites , Prostheses and Implants , Animals , Bone Marrow/pathology , Bone and Bones/pathology , Durapatite , Fibroblasts/pathology , Hydroxyapatites/chemistry , Osteitis/pathology , Osteogenesis , Rabbits , Tibia , Wound HealingABSTRACT
UVA-radiation can produce malignant changes in skin. When making light cured dental fillings, dentist and dental assistant, particularly on their fingers, may be exposed to UVA radiation. The latex examination gloves were able to absorb 76% of the radiation, whereas the vinyl examination gloves absorbed only 33% of the radiation. Latex gloves thus proved to give more effective protection against UVA radiation than vinyl gloves.
Subject(s)
Gloves, Surgical , Latex , Ultraviolet Rays , Vinyl Compounds , Absorption , Materials Testing , Radiation Dosage , Random AllocationABSTRACT
The minimal phototoxic dose values for UVA radiation of psoralen-treated skin and of oral mucous membrane were studied in 16 healthy volunteers. A commercial 0.01% trioxalen ointment was used as the topical photosensitizer. In all 16 persons the radiation dose needed to induce erythema was greater for the buccal mucosa than for the skin, and the average buccal minimal phototoxic dose was 2.3-fold that of the cutaneous minimal phototoxic dose.
Subject(s)
Mouth Mucosa/drug effects , PUVA Therapy , Skin/drug effects , Trioxsalen/toxicity , Administration, Topical , Adult , Female , Humans , Lichen Planus/drug therapy , Male , Mouth Diseases/drug therapy , Trioxsalen/administration & dosage , White PeopleABSTRACT
A botryoid odontogenic cyst (BOC) which recurred 4 times over a period of 9 years is presented. Immunocytochemical comparison of cytokeratin composition of the cyst epithelium with that of the overlying oral epithelium showed distinct differences. The cyst epithelium expressed keratins typical of both simple and nonkeratinizing squamous epithelia. The keratin profile of BOC is in line with previous findings on odontogenic epithelia, thus confirming its odontogenic origin.
Subject(s)
Mandibular Diseases/pathology , Odontogenic Cysts/pathology , Epithelium/analysis , Epithelium/pathology , Female , Humans , Immunohistochemistry , Keratins/analysis , Mandibular Diseases/metabolism , Middle Aged , Odontogenic Cysts/analysis , RecurrenceABSTRACT
Seventeen patients with histologically confirmed oral lichen planus (LP) unresponsive to previous treatments were enrolled in a study on the feasibility of photochemotherapy in the treatment of oral LP. An Espe Uviolite apparatus originally designed for irradiation of light-cured dental composite fillings was used as the source of radiation. The wavelength range of the apparatus was 320-400 (UVA) nm. Two hours before irradiation, 8-methoxy-psoralen (0.6 mg/kg) was administered orally, treatments being given at intervals of 2-3 days. Subjective symptoms disappeared totally in 13 of 17 patients at a mean cumulative dose of 6.1 J/cm2. Two months after treatment with a mean total dose of 11.6 J/cm2, 12 of the 17 lesions had markedly improved. After 12-24 months of follow-up, complete clinical remission was found in 5 of the 17 patients, 7 patients were in partial remission and 5 remained only slightly improved. We conclude that PUVA treatment may be useful for treating recalcitrant oral LP but, until more extensive studies are performed, it must be considered as experimental.
