ABSTRACT
Conditions for the submerged cultivation of a strain of V. cholerae O139, were worked out. These conditions ensured a high yield of biomass, soluble O-antigen and exoenzymes (proteinase, phospholipase A) into the culture medium, which exceeded their production by strains of serovar O1, respectively, 2, 3, 4 and 8 times. The preparation, isolated from the culture fluid and lyophilized, contained up to 50% of O-antigen and exoenzymes. In experiments on white mice the preparation exhibited low toxicity (LD50 was equal, on the average, to 1.2 mg) and immunogenicity (ED50 was equal to 3-5 micrograms) with respect to V. cholerae O139, which corresponded to the protective potency of commercial vaccine against V. cholerae O1 infection.
Subject(s)
Bacterial Vaccines/biosynthesis , Vaccines, Synthetic/biosynthesis , Vibrio cholerae/immunology , Animals , Antigens, Bacterial/immunology , Antigens, Bacterial/isolation & purification , Antigens, Bacterial/toxicity , Bacterial Vaccines/immunology , Bacterial Vaccines/isolation & purification , Bacterial Vaccines/toxicity , Culture Media , Epitopes/immunology , Epitopes/isolation & purification , Epitopes/toxicity , Immunization , Lethal Dose 50 , Mice , Rabbits , Vaccines, Synthetic/immunology , Vaccines, Synthetic/isolation & purification , Vaccines, Synthetic/toxicity , Vibrio cholerae/growth & developmentABSTRACT
The influence of Y. pestis phospholipase D on the physiological state of leukocytes in the blood of guinea pigs was studied in vivo by flow impulse fluorometry with the use of fluorochrome acridine orange. During the first hours of observation the intensity of leukocyte fluorescence increased due to a rise in the number of polymorphonuclear leukocytes and changes in the permeability of cell membranes. Further changes in the intensity of the fluorescence of the material under study after 24 hours of observation occurred due to the appearance of activated lymphocytes in the blood stream. The processes normalized by day 21. The reaction of blood leukocytes to phospholipase D was specific in comparison with the reaction to capsular antigen, "mouse" toxin, lipopolysaccharide and the main somatic antigen.
