ABSTRACT
AIM: to define a role of hepatotropic (HAV, HBV, HCV, and HDV) and opportunistic hepatotropic (HGV, CMV, EBV, HHV types 1, 2, and 6) viruses in the etiological pattern of diseases accompanied by enhanced blood AlAT and AsA T activities in pregnant women. SUBJECTS AND METHODS: Two hundred and eleven pregnant women, including 123 patients with chronic viral hepatitis, 74 with enhanced blood AlAT activity and no markers of viral hepatitis (EAlA T-NMVH), and 14 with acute viral hepatitis were examined. RESULTS: Most pregnant women with chronic HBV and HCV infections were found to have HBV DNA and HCV RNA in the blood in the presence of normal and enhanced activities of transaminases. In the EAlAT-NMVH group, there was none of the opportunistic hepatotropic viruses in more than 7% of cases. No genetic material of HAV, HBV, HCV, HDV, HGV, CMV, EBV, HHV types 1, 2, and 6 was found in the blood of all 10 patients with hepatitis of unspecified etiology. CONCLUSION: In the absence of serologic data supporting the presence of infectious pathology, blood testing using the polymerase chain reaction is of low informative value in detecting opportunistic hepatotropic viruses in pregnant women with hepatitis of unspecified etiology. However, by keeping in mind that the spectrum of opportunistic hepatotropic viruses is not confined to those included in this study, it is expedient to examine additionally pregnant women with enhanced blood AlAT and AsAT activity in order to identify TTV, B19V, HHV-8, SEN and NV-F in the blood.
Subject(s)
Alanine Transaminase/blood , Aspartate Aminotransferases/blood , Hepatitis, Viral, Human/virology , Opportunistic Infections/virology , Pregnancy Complications, Infectious/virology , Female , Hepatitis, Viral, Human/blood , Hepatitis, Viral, Human/enzymology , Hospitals, Special , Humans , Liver Function Tests , Obstetrics and Gynecology Department, Hospital , Opportunistic Infections/blood , Opportunistic Infections/enzymology , Pregnancy , Pregnancy Complications, Infectious/blood , Pregnancy Complications, Infectious/enzymology , Prospective StudiesABSTRACT
Moscow infection hospital N 1 admitted in 1970-2000 fifty seven pregnant women with acute fatty gestational hepatosis (AFGH). 10 women died. AFGH is a severe complication of pregnancy. It occurs rather rarely, has clinical variants, can be mistaken for infectious diseases, primarily for viral hepatitides. When AFGH diagnosis is verified, the patient must immediately deliver otherwise the prognosis is poor.
Subject(s)
Fatty Liver/diagnosis , Jaundice/diagnosis , Pregnancy Complications/diagnosis , Adult , Diagnosis, Differential , Fatty Liver/complications , Female , Hepatitis, Viral, Human/diagnosis , Humans , Jaundice/complications , Pregnancy , PrognosisABSTRACT
AIM: To evaluate course and outcomes of hepatitis C in gravidae; introduction of optimal management of pregnancy, postpartum period in mothers and newborns in viral hepatitis C. MATERIAL AND METHODS: Case histories, general and obstetric statuses, obstetric and extragenital pathology, pregnancy course and outcomes were studied in 76 pregnant women with viral hepatitis C. RESULTS: A complicated course of pregnancy was observed in 38.2, 13.1 and 24.3% of women in trimester I, II and III, respectively. Therapeutic policy of removing symptoms of the pathology with consideration of hepatitis C severity and stage allowed to keep pregnancy in all the women. CONCLUSION: Hepatitis C virus can complicate pregnancy but the latter does not aggrevate the course of hepatitis C.
Subject(s)
Hepatitis C , Pregnancy Complications, Infectious , Adult , Antiviral Agents/therapeutic use , Diagnosis, Differential , Female , Hepacivirus/genetics , Hepacivirus/immunology , Hepatitis C/diagnosis , Hepatitis C/drug therapy , Hepatitis C/virology , Hepatitis C Antibodies/analysis , Humans , Pregnancy , Pregnancy Complications, Infectious/diagnosis , Pregnancy Complications, Infectious/drug therapy , Pregnancy Complications, Infectious/virology , Pregnancy Outcome , Prognosis , RNA, Viral/analysis , Severity of Illness IndexABSTRACT
A new procedure for colorimetric determination of the dehydrogenase activity in the mycelium of Streptomyces levoris was developed. The procedure is more precise, universal and adaptive. The novelty of the procedure is in the switching of the respiration chain to ascorbic acid that excludes the use of the respiration chain inhibitors. The adaptivity refers to the adjusting of the reaction to a particular strain by the reaction time and mycelium concentration in the reaction mixture. The procedure is technologically advantageous and handy. It provides simultaneous assay of a large number of samples of different type dehydrogenases.
Subject(s)
Citric Acid Cycle/physiology , Oxidoreductases/analysis , Streptomyces/enzymology , Adaptation, Physiological , Colorimetry , Electron Transport/physiology , Streptomyces/ultrastructureABSTRACT
The influence of succinic acid as a component of media for biosynthesis of levorin, a polyenic antibiotic was studied. It was shown that with the use of the soybean-corn medium supplemented with succinic acid (0.05-0.4 per cent) the antibiotic content in the fermentation broth was higher than that in the control. The highest stimulating effect (135 per cent) was observed with addition of 0.1 per cent of succinic acid. For providing optimal antibiotic production in the synthetic medium supplemented with succinic acid (0.4 per cent) addition of acetic acid (0.05 per cent) was required. Studies with the soybean-corn medium with and without succinic acid revealed differences in the level of p-aminoacetophenone, an aromatic fragment of the levorin molecule. Under the conditions of the medium with succinic acid the content of p-aminoacetophenone in the mycelium was higher by 10 to 18 per cent as compared to that in the control and depended on the fermentation period. The role of succinic acid in biosynthesis of levorin is discussed.
