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1.
Radiats Biol Radioecol ; 54(2): 127-39, 2014.
Article in Russian | MEDLINE | ID: mdl-25764814

ABSTRACT

The study of aberrant methylation of CpG islands in the promoter regions of genes (P16/CDKN2A, P14/ARF, RASSF1A, GSTP1) in blood leukocytes of liquidators of the Chernobyl accident (n = 83, 38-76 years of age) and control subjects of two groups (n = 48, age ≤ 35 and n = 65, age > 35) was carried out using methylation-sensitive restriction endonuclease analysis followed by PCR. The total number of AciI sites in the analyzed fragments ranged from 2 to 7 for different genes. Only 1 subject (2.1%) from the control group (healthy young individuals, age ≤ 35) has methylation of the studied CpG--dinucleotides of RASSF1A gene. Promoter methylation of at least one of the genes analyzed was observed in 28.92% liquidators and significantly exceeded (p = 0.016) such rate in a one-age (> 35 years of age) control group (12.31%). A significantly elevated frequency (p = 0.023) of individuals with abnormal methylation of GSTP1 gene in the group of liquidators as compared to the control group was revealed. The occurrence of promoter methylation of RASSF1A gene significantly correlated with aging both in the control group (r = 0.214; p = 0.023) and in the liquidators of the Chernobyl accident (r = 0.230; p = 0.036). No similar trend was found for other genes. Multiple regression analysis showed that the growth in the number of methylated loci of a set of genes p16, p14 and GSTP1 is exclusively due to the fact of exposure (OR = 7.32, 95% CI = 2.49-25.83, p-value = 2.7 x 10(-5)). The results obtained demonstrate for the first time the reality of the radiation-induced aberrant methylation of CpG islands in promoters of genes involved in the basic protective, functions of cells in the human body in remote periods after radiation exposure.


Subject(s)
CpG Islands/radiation effects , DNA Methylation/radiation effects , Leukocytes/radiation effects , Promoter Regions, Genetic/radiation effects , Adult , Aged , Chernobyl Nuclear Accident , Cyclin-Dependent Kinase Inhibitor p16/genetics , DNA Methylation/genetics , Female , Glutathione S-Transferase pi/genetics , Humans , Male , Middle Aged , Tumor Suppressor Proteins/genetics
2.
Article in Russian | MEDLINE | ID: mdl-21446169

ABSTRACT

AIM: To obtain monoclonal antibodies (MCAs) to glycoprotein E1 of rubella virus, to assess their immunochemical characteristics and ability to use fluorescent MCA for rapid identification of rubella virus. MATERIALS AND METHODS: Rubella virus strain C-74 (Moscow), vaccine strains "Orlov" (Saint-Petersburg), Wistar RA 27/3 (USA) as well as strain Judith (Germany) were used. Viral antigens were obtained using diploid cells L-68 and cell lines VNK-21-F and Vero E6. MCAs were produced by conventional method and their isotype was determined: Immunoblotting, immunoenzume assay (IEA), hemagglutination inhibition assay (HIA) and immunofluorescence assay (IFA) were performed. RESULTS: Five monoclonal antibodies--Kh-252.1, Kh-347.2, Kh-183.3, Kh-214.4, Kh-187.5--to antigens of rubella virus strain C-74 were obtained. Isotypes of these antibodies were determined and their reactivity with native and denaturated antigens of other strains ("Orlov", Wistar RA 27/3, Judith) was characterized. IEA showed that all MCAs interacted with rubella virus glycoprotein E1 at high titers ranging from 1/1600 to 1/200,000. Immunoblotting demonstrated that 4 MCAs (Kh-252.1, Kh-347.2, Kh-183.3, Kh-214.4) had aforementioned feature. MCAs inhibited hemagglutinating activity of Judith strain in titer from 1/16 to 1/1024 in HIA. FITC conjugate of MCA Kh-347.2 (most sensitive variant) allowed to detect rubella virus in infected Vero E6 cells after 24 hours since infection, whereas FITC conjugates of 3 MCAs (Kh-183.3, Kh-214.4, Kh-187.5)--after 72 hours since infection. CONCLUSION: Use of FITC conjugates of MCAs is a perspective tool for identification of rubella virus glycoprotein E1 in infected cell cultures and nasopharyngeal swabs.


Subject(s)
Antibodies, Monoclonal, Murine-Derived/immunology , Rubella virus/immunology , Viral Envelope Proteins/immunology , Animals , Antibodies, Monoclonal, Murine-Derived/chemistry , Chlorocebus aethiops , Humans , Mice , Mice, Inbred BALB C , Rubella/diagnosis , Rubella/immunology , Vero Cells
3.
Radiats Biol Radioecol ; 50(2): 128-33, 2010.
Article in Russian | MEDLINE | ID: mdl-20464958

ABSTRACT

The genes of detoxication, MTHFR and p53 were studied in Down' and Ehlers-Danlos syndrome cells. The frequency GSTM1(0/0) genotype in Down syndrome patients was in 1.5 times higher than in control cells (p < 0.069). Opposite the frequency GSTM1(0/0) genotype in Ehlers-Danlos syndrome was 23.3% 2 times lower than in control cells (p < 0.034). This indication was in 2 times lower in women cells than in men cells and in 3 times lower than in control cells (p < 0.026). The mutations of p53 gene (7th exon) were detected in 4 from 11 Down patients (36.7%; in 2 cases af women and men), in Ehlers-Danlos patients--in 5 cases and only in men (29.4% among all the observed patients). The observations 24 healthy donors weren't revealed any mutations (p < 0.013-0.001). The hypothesis about the connection between gene polymorphisms which take a part in genome stability and radiosensitivity in Down and Ehlers-Danlos patients was developed.


Subject(s)
Down Syndrome/genetics , Ehlers-Danlos Syndrome/genetics , Methylenetetrahydrofolate Reductase (NADPH2)/genetics , Radiation Tolerance/genetics , Tumor Suppressor Protein p53/genetics , Adolescent , Child , Child, Preschool , Female , Gene Frequency , Humans , Male , Polymorphism, Genetic
4.
Radiats Biol Radioecol ; 49(5): 543-51, 2009.
Article in Russian | MEDLINE | ID: mdl-19947517

ABSTRACT

Here presented the data on the frequencies of chromosome aberrations in lymphocytes of peripheral blood of 97 volunteers depending on genotypes by genes of xenobiotics detoxication before and after gamma-irradiation with dose of 1 Gy in vitro. The frequencies of aberrations were estimated by analyzing not less than 500-1000 metaphases per person. The data of cytogenetic analysis were compared with the results of PCR-genotyping of loci GSTM1, GSTT1, GSTP1, CYP1A1, CYP2D6, NAT2, and MTHFR. The significant differences by the frequencies of aberrations between "single-locus" genotypes were not found except for GSTM1 locus, for which the enhanced frequency of spontaneous aberrations of chromosome type in "positive" genotypes compared to "zero" ones, i.e., homozygotes by deletion (p = 0.04) was observed. The minimum frequency of spontaneous aberrations of chromosome type was recorded for carriers of double homozygotes by deletion of GSTM1-GSTT1: 0.0006 +/- 0.0003 against 0.0027 +/- 0.0003 for the rest of genotypes (p = 0.016 by the Mann-Witney test). The frequency of gamma-induced chromosome aberrations was correlated with the total amount of minor alleles in loci GSTP1, NAT2, and MTHFR (r = 0.25 at p = 0.0065).


Subject(s)
Arylamine N-Acetyltransferase/genetics , Chromosome Aberrations , Gamma Rays , Glutathione Transferase/genetics , Methylenetetrahydrofolate Reductase (NADPH2)/genetics , Polymorphism, Genetic , Adult , Alleles , Cells, Cultured , Humans , Lymphocytes/radiation effects , Xenobiotics/metabolism
5.
Genetika ; 45(6): 753-7, 2009 Jun.
Article in Russian | MEDLINE | ID: mdl-19639866

ABSTRACT

Cells of a diploid line obtained from embryos with the Down's syndrome, known to be unable to repair gamma-induced DNA damage, were treated with natural (garlic extract, retinol) and synthetic (crown compound) antimutagens and with adapting factors (heat shock, low CdCl2 concentrations, 10(-8) M). The protective effect was evaluated by registering DNA breaks and cell survival, and the protection coefficients were calculated. The most effective results were obtained with the use of the garlic extract and retinol. No protection of the DNA structure was observed when cells were treated with low concentrations of cadmium chloride and then with high concentrations, i. e., no adaptive response (AR) was formed under these conditions. The spectrum of proteins in treated and control cells as well as detoxication genes (GSTM1, GSTT1 , CYPIA1) were determined.


Subject(s)
Antimutagenic Agents/pharmacology , Cadmium Chloride/toxicity , DNA Damage/physiology , DNA Repair , Mutagens/toxicity , Polymorphism, Genetic , Adaptation, Physiological , Cadmium Chloride/pharmacology , Cell Line , Crown Compounds/pharmacology , Cytochrome P-450 CYP1A1/genetics , Cytochrome P-450 CYP1A1/metabolism , DNA Damage/drug effects , DNA Damage/radiation effects , Down Syndrome/pathology , Fibroblasts/drug effects , Fibroblasts/physiology , Fibroblasts/radiation effects , Garlic/chemistry , Glutathione Transferase/genetics , Glutathione Transferase/metabolism , Hot Temperature , Humans , Plant Extracts/pharmacology , Vitamin A/pharmacology
6.
Article in Russian | MEDLINE | ID: mdl-19491810

ABSTRACT

Polymorphisms of glutation-S-transferase (GSTM1, GSTT1 GSTP1) and methylentetrahydrofolate reductase (MTHFR) genes have been studied in DNA from blood lymphocytes of 18 patients with Down's syndrome and 61 controls. Frequencies of normal alleles of GST genotypes were lower in patients as compared to the controls. A DNA analysis of 11 patients and 17 controls revealed the presence of mutations in region 246-250 of exon 7 of the p53 gene in 4 patients. Mutations were not found in the control group. Due to the small sample size, the results of this study should be interpreted with caution and need replication in larger studies.


Subject(s)
DNA/genetics , Down Syndrome/genetics , Glutathione Transferase/genetics , Methylenetetrahydrofolate Reductase (NADPH2)/genetics , Polymorphism, Genetic , Adult , Alleles , Child , Down Syndrome/enzymology , Gene Frequency , Genotype , Glutathione Transferase/blood , Humans , Methylenetetrahydrofolate Reductase (NADPH2)/blood , Mutation
7.
Radiats Biol Radioecol ; 48(3): 278-86, 2008.
Article in Russian | MEDLINE | ID: mdl-18689252

ABSTRACT

A complex of cytogenetic and of immunogenetic study of the state of the lymphocyte genomes in the liquidators of the ChNPP accident and their unirradiated children has been carried out for the first time. Increased frequencies of the chromosome aberrations, of gene mutations (TCR mutations) and of predictors of apoptosis (cells with CD95+ immunophenotype) have been revealed in both generations. The analysis of correlations between the parameters under study has demonstrated distinctive features characteristic of induction of genomic instability in the organism of unirradiated children as compared to their fathers--liquidators directly exposed to radiation. Individual variability of genome destabilization were observed by all criteria used and manifested themselves in the diverse spectrum of transgenerational mutational effects and in different levels of their expression. The results obtained demonstrate the necessity of integral evaluation of the state of the genome using several genetic criteria to reveal transgenerational genomic instability in children of a special category--the offsprings of irradiated parents.


Subject(s)
Chernobyl Nuclear Accident , Chromosomal Instability/genetics , Chromosomal Instability/immunology , Paternal Exposure , Radiation Injuries/genetics , Radiation Injuries/immunology , Radioactive Hazard Release , Adolescent , Adult , Child , Child, Preschool , Cytogenetic Analysis , Dose-Response Relationship, Radiation , Genome, Human/radiation effects , Humans , Lymphocytes/immunology , Lymphocytes/radiation effects , Male , Mutation , Occupational Exposure/adverse effects , Radiation, Ionizing , Receptors, Antigen, T-Cell/genetics , Receptors, Antigen, T-Cell/radiation effects , Ukraine , fas Receptor/immunology
8.
Radiats Biol Radioecol ; 48(3): 303-12, 2008.
Article in Russian | MEDLINE | ID: mdl-18689254

ABSTRACT

The dependence of the level of unstable chromosome aberrations and nononcological diseases on the genotype in 57 liquidators of the ChNPP accident was studied. Candidate genes presumably affecting radiosensitivity were highly polymorphic loci of xenobiotic detoxication genes (glutathione-S-transferases GSTM1, GSTT1, GSTP1) and the 5,10-methylenetetrahydrofolate reductase gene (MTHFR) involved in DNA methylation and synthesis. An increased frequency (0.014 +/- 0.001 per cell) of unstable chromosome aberrations, including radiation-specific dicentrics and centric rings (0.0015 +/- 0.0002 per cell), has been found to be preserved in the group of liquidators examined in 2006-2007. No associations of polymorphism for each of the studied genes with cytogenetic parameters were revealed. Increased frequencies of chromosome aberrations were recorded in homozygous carriers of a deletion at the GSTM1 locus in combination with homozygosity for minor alleles at the MTHFR and GSTP1 loci (p = 0.00002 and p = 0.0233, respectively). The number of homozygous carriers of the minor allele GSTP1 was increased among patients with chronic obstructive pulmonary disease and in liquidators with acute circulation disturbances (p = 0.014 and p = 0.04, respectively). Double homozygotes for GSTM1 and GSTT1 deletions were significantly more frequent among subjects with benign tumors (cysts, polyps, p = 0.015) and with benign thyroid tumors (p = 0.017). This genotype has proved to be protective for patients with severe cardiovascular diseases (acute circulation disturbances, p = 0.027).


Subject(s)
Chernobyl Nuclear Accident , Chromosome Aberrations , Occupational Exposure/adverse effects , Radiation Injuries/complications , Radiation Injuries/genetics , Radiation Tolerance/genetics , Radioactive Hazard Release , Alleles , Female , Gene Frequency , Genotype , Glutathione S-Transferase pi/genetics , Glutathione Transferase/genetics , Homozygote , Humans , Male , Methylenetetrahydrofolate Reductase (NADPH2)/genetics , Middle Aged , Point Mutation , Polymorphism, Genetic , Pulmonary Disease, Chronic Obstructive/epidemiology , Pulmonary Disease, Chronic Obstructive/etiology , Pulmonary Disease, Chronic Obstructive/genetics , Sequence Deletion , Thyroid Neoplasms/epidemiology , Thyroid Neoplasms/etiology , Thyroid Neoplasms/genetics , Vascular Diseases/epidemiology , Vascular Diseases/etiology , Vascular Diseases/genetics
9.
Radiats Biol Radioecol ; 48(6): 690-7, 2008.
Article in Russian | MEDLINE | ID: mdl-19178046

ABSTRACT

Mini/microsatellite (MNS/MCS) loci are efficient tools in solving basic and applied problems in different spheres of biology and medicine due to their unique characteristics - a high frequency of tandem repeats in combination with their wide variability. Specifically, they have been found use as potential markers of genetic effects of ionizing radiation on animals and on human. However there is no general agreement as to the influence of irradiation on the frequency of mutations in hypervariable repetitive DNA sequences up to now. The present work is the study of the mutation frequency at MCS/MNS loci in 19 families of workers occupationally exposed to chronic beta-radiation from tritium and tritium oxide (examined group), and in the control group included 23 families. The results have indicated that the average frequency of microsatellite mutations in the examined group made up 4.7% and exceeds about 7-fold the same parameter of the control group (0.7%). This differences is statistically significant (p = 0.004). The average frequency of minisatellite mutation in the examined group made up 3% while in the control group it was 2 time lower (1.5%), but this difference is not statistically significant. Mutations for 4 MCS and 2 MNS loci were revealed in two children from one family (the total reconstructed dose in their father was about 1000 mSv). If we exclude this family from statistical analysis the frequencies of MCS and MNS mutations in the children of nuclear workers do not statistically differ from the control values.


Subject(s)
Microsatellite Repeats/genetics , Mutation , Occupational Exposure , Tritium/toxicity , Adolescent , Adult , Aged , Beta Particles , Child , DNA Mutational Analysis , Family Health , Female , Germ Cells/radiation effects , Humans , Male , Middle Aged , Paternal Exposure , Polymorphism, Genetic , Radiation Dosage , Water
10.
Bull Exp Biol Med ; 144(5): 717-21, 2007 Nov.
Article in English | MEDLINE | ID: mdl-18683505

ABSTRACT

Study of polymorphism in 3 genes of the glutathione S-transferase family (GSTM1, GSTT1, and GSTP1) in children with Ehlers-Danlos syndrome whose cells were defective in repair of gamma-induced DNA damages revealed accumulation of GSTM1(+) genotypes compared to children of the control group. Generation of reactive oxygen species by neutrophils from patients with this syndrome was higher than in healthy donors. Our results indicate that glutathione S-transferase genes are involved in the resistance to mutagenic agents and demonstrate medical and genetic peculiarities of patients with Ehlers-Danlos syndrome.


Subject(s)
Ehlers-Danlos Syndrome/genetics , Lymphocytes/metabolism , Mutagens/pharmacology , Polymorphism, Genetic , Adolescent , Cadmium Chloride/pharmacology , Child , DNA Damage , Drug Resistance , Ehlers-Danlos Syndrome/blood , Female , Gamma Rays , Gene Frequency , Genetic Predisposition to Disease/genetics , Genotype , Glutathione Transferase/genetics , Humans , Lymphocytes/drug effects , Lymphocytes/radiation effects , Male , Reactive Oxygen Species/metabolism
11.
Radiats Biol Radioecol ; 46(4): 424-8, 2006.
Article in Russian | MEDLINE | ID: mdl-17020091

ABSTRACT

A complex investigation of different cell defence systems, such as: DNA repair, antioxidant system (SOD), xenobiotic detoxification system (glutathione-S-transferases M1 and T1), radioadaptive response (RAR) in lymphocytes of patients with hereditary disease of connective tissue (Elers-Danlose syndrome) was carried out. The frequency of genotype GSTM1 (0/0) in children with Elers-Danlose syndrome (23%) is lower as compared to the control group (44%). The lymphocytes of children with Elers-Danlose syndrome were characterized by reduced ability to repair gamma-induced damage of DNA. At given size of the samples of examined children no correlative relationships between GST-status of organism and the condition of other cell defence systems were revealed. The data obtained demonstrate the individual peculiarities of the defence systems in repair-deficient cells of the examined children.


Subject(s)
DNA Repair/genetics , Ehlers-Danlos Syndrome/genetics , Glutathione Transferase/genetics , Child , DNA/analysis , Ehlers-Danlos Syndrome/enzymology , Female , Humans , Lymphocytes/enzymology , Male , Polymorphism, Genetic
12.
Radiats Biol Radioecol ; 46(4): 466-74, 2006.
Article in Russian | MEDLINE | ID: mdl-17020099

ABSTRACT

A complex genetic examination of children which belong to two cohorts and their parents were carried out. The first cohort included children and constantly living on territories contaminated with radionuclides (Novozybkov district, Bryansk region). They were subdivided in groups according to the ontogenetic age periods of development of their parents at the time of the Chernobyl accident. In the children born in 1986-1995 the level of aberrant genomes is significantly higher as compared to the control (p < 0.001). In children born in 1998-2002 the differences are insignificant (p > 0.05). The frequency of aberrant genomes had a tendency to decrease with the period of time between the birth date of a child and the moment of the accident. Analysis of the results of cytogenetic investigation for the same living on territories with different densities of radioactive contamination (zone I-- 627-688 kBq/m2, 137Cs and zone II-- 135-402 kBq/m2, 137Cs) revealed insignificant differences in the spectrum and average frequencies of chromosome aberrations. The second cohort included children born in 1987-1991 and 1993-2002 from irradiated fathers (Chernobyl clean-up workers) and unirradiated mothers living on territories without radionuclide contamination. These children also displayed increased frequencies of aberrant genomes as compared to the control (p < 0.001). The analysis of the dynamics years of birth of cytogenetic disturbances in the same cohorts of children showed the average frequencies of aberrant genomes remain higher than the control level. In most of the children of both cohorts the repair synthesis of genome DNA by gamma- and UV-radiation is reduced as compared to one in the children from the control group.


Subject(s)
Chernobyl Nuclear Accident , Chromosome Aberrations , Genomic Instability , Radioactive Hazard Release , Adolescent , Cesium Radioisotopes , Child , Child, Preschool , Chromosomes, Human/radiation effects , Cytogenetic Analysis , DNA Repair , Environmental Exposure , Female , Gamma Rays , Health , Humans , Lymphocytes/cytology , Male , Radioactive Pollutants/toxicity
13.
Radiats Biol Radioecol ; 46(2): 167-77, 2006.
Article in Russian | MEDLINE | ID: mdl-16756114

ABSTRACT

The main results of the complex examination of the genome instability are presented in children constantly living on territories contaminated with radionuclides as a result of the accident at the CNPP (Novozybkov district, Bryansk region, 16-18 Ci/km2, 137Cs) and in children exposed to low-intensity radiation at different stages of ontogenetic development: children exposed to postnatal irradiation in 1986 (born before the accident), children exposed to intrauterine irradiation during the accident in 1986, children of irradiated parents born after the accident in 1987-1992 and in 1994-2000. In all examined groups of irradiated children increased frequencies of certain radiation-induced chromosome aberrations were observed as well as a reduced activity of unscheduled synthesis of genomic DNA in lymphocytes and peculiarities in individual heterozygosity of genes encoding structural and enzymatic proteins of blood. An increased radiosensitivity of lymphocyte genomes to testing in vitro irradiation and peculiarities in the dynamics of the frequencies of chromosome aberrations and sister chromatid exchanges in 3 cell generations were revealed in children from the contaminated areas. The data obtained suggest a systemic character of dysgenomic effects, the reality of induction of genome instability in the growing organism of children exposed to low-intensity radiation at low doses the expression of which is determined by individual genotypic features of the organism. Biological significance of the phenomenon of the post-radiation genome instability, its relation to the state of health and the pathogenetic role in the development of somatic pathology are postulated.


Subject(s)
Chernobyl Nuclear Accident , Disease/etiology , Genomic Instability , Power Plants , Radioactive Hazard Release , Adolescent , Blood Proteins/drug effects , Blood Proteins/genetics , Child , Child, Preschool , Chromosome Aberrations , DNA/biosynthesis , Dose-Response Relationship, Radiation , Environmental Exposure , Female , Heterozygote , Humans , Infant, Newborn , Lymphocytes/physiology , Lymphocytes/radiation effects , Male , Maternal Exposure , Morbidity , Paternal Exposure , Pregnancy , Prenatal Exposure Delayed Effects , Russia/epidemiology , Soil Pollutants, Radioactive , Ukraine
14.
Radiats Biol Radioecol ; 43(2): 150-2, 2003.
Article in Russian | MEDLINE | ID: mdl-12754797

ABSTRACT

A concept of polygenomic realization of mutagenic effects in the human body exposed to low-dose radiation on the basis of cell reproductions of genomic damages (Nig = 2n/2) was suggested. The above-said is in agreement with the principles of non-threshold mutagenic action of radiation and biological amplifier, and lead to the development of polygenomic dysbalance with pathophysiological consequences.


Subject(s)
Chromosome Aberrations , Mutagenesis , Radiation Genetics , Radioactive Hazard Release , Age Factors , Child , Follow-Up Studies , Humans , Power Plants , Radiation Dosage , Russia , Time Factors , Ukraine
16.
Radiats Biol Radioecol ; 42(6): 735-9, 2002.
Article in Russian | MEDLINE | ID: mdl-12530161

ABSTRACT

An experimental study of expression of genomic instability was carried out with the use of testing irradiation in peripheral blood lymphocytes of 15 children (born in 1986-1998) living on territories with radionuclide contaminations (over 15 Ci/km2, 137Cs, Novozybkov district, Bryansk region). In 5 children exposed to intrauterine irradiation in 1986 this phenomenon was studied in 3 successive cell generations (mitoses). The data obtained suggest the reality of expression of induced genomic instability in the offsprings of repeatedly divided cells of a growing organism exposed to prolonged action of low radiation levels.


Subject(s)
Cesium Radioisotopes/pharmacology , Genome, Human , Lymphocytes/radiation effects , Child , Female , Humans , Lymphocytes/ultrastructure , Male , Maternal Exposure , Pregnancy
17.
Article in Russian | MEDLINE | ID: mdl-12525007

ABSTRACT

The content of total IgE, antibodies to thyroglobulin (TG-Ab), antibodies to thyreoid peroxidase (TPO-Ab) in the blood serum and skin reaction to autologous serum were detected in patients with chronic relapsing urticaria (CRU). The skin test to autologous serum yielded positive results in 42% of the patients. The elevated levels of TG-Ab and TPO-Ab were detected in 30.7% and 35.4% of the patients, the elevated level of total IgE was detected in 60% of the patients. At the same time the detection rates of antithyreoid antibodies and the elevated level of IgE were not linked with skin reaction to autologous serum. Apparently, in addition to autoantibodies to IgE or its receptor (the positive skin test to autologous serum), thyroid gland antibodies may take part in the mechanism of the CRU formation.


Subject(s)
Autoantibodies/blood , Thyroid Gland/immunology , Urticaria/immunology , Adolescent , Adult , Autoantigens/immunology , Chronic Disease , Female , Humans , Immunoglobulin E/blood , Iodide Peroxidase/immunology , Male , Middle Aged , Recurrence , Skin Tests , Thyroglobulin/immunology , Urticaria/blood
18.
Radiats Biol Radioecol ; 41(5): 606-14, 2001.
Article in Russian | MEDLINE | ID: mdl-11721354

ABSTRACT

The phenomenological aspects of the genomic instability induced in the descendants of the multi-divided cells having been exposed to the radiation are examined. It is demonstrated that the regularity of the chromosome instability induction do not correspond to the classical conception of the radiation genetics (hit principle and target theory). The mechanisms and the biological significance of this new genetic phenomenon in the child organism under conditions of low-intensive effect of small-dose radiation and its connection with the state of health are discussed.


Subject(s)
Chromosomes/radiation effects , Power Plants , Radiation Injuries/genetics , Radioactive Hazard Release , Adult , Age Factors , Animals , Bone Marrow Cells/radiation effects , Cells, Cultured , Child , Chromosome Aberrations , Cricetinae , Cricetulus , DNA/radiation effects , Female , Gamma Rays , Genome , Humans , Male , Mice , Mutation , Paternal Exposure , Radiation Dosage , Rats , Time Factors , Ukraine , Ultraviolet Rays
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