ABSTRACT
IgM, IgG antibodies to herpes simplex virus and their subclases were investigated in 565 subjects of different age tested at virological laboratories of St. Petersburg in 1996-1997. The majority of these subjects had a history of herpes infection and 21.5% had IgM antibodies to herpes simplex virus (HSV), marker of acute herpetic infection. Besides IgM, IgG1 antibodies can be referred to early antibodies appearing during the acute stage of herpetic infection. The predominant subclass was HSV IgG3 antibodies. As for IgG4, they were completely absent in infants aged under 1 year, were detected in 6.2% children aged under 14 years, and were present in 12.2-12.5% adults.
Subject(s)
Antibodies, Viral/immunology , Immunoglobulin G/immunology , Immunoglobulin M/immunology , Simplexvirus/immunology , Adolescent , Adult , Child , Child, Preschool , Humans , Immunoglobulin G/classification , InfantABSTRACT
The incidence of cytomegalovirus (CMV) infection was assessed from the presence of anti-CMV IgM in the sera of 1312 children of different age and 250 adults by the Labsystems enzyme immunoassay kit. CMV-specific IgM are rare in newborns (1.3%), but their incidence increases to 5% during the first year of life. These antibodies occur in 5-6% preschool and schoolchildren. The incidence of acute CMV infection is the highest in adults: 15%.
Subject(s)
Cytomegalovirus Infections/epidemiology , Adolescent , Adult , Child , Child, Preschool , Cytomegalovirus/immunology , Humans , Immunoenzyme Techniques , Immunoglobulin M/immunology , Incidence , Infant , Infant, Newborn , Russia/epidemiologyABSTRACT
Enzyme immunoassay for the detection of IgM antibodies to human cytomegalovirus (CMV) has been developed. Use of horseradish peroxidase conjugated monoclonal antibodies to human IgM helped create a highly specific test system. The rheumatoid factor was removed from the serum by staphylococcal reagent containing protein A. The results of the newly developed assay are in good correlation with the clinical course of the disease and data of polymerase chain reaction and the test making use of the Labsystem. Cytomegalovirus IgM EIA Kit.
