Serum Activity of Proteolytic Enzyme Trypsin in Rats under Conditions of Water and Food Deprivation.

Trypsin is mainly regarded as a digestive enzyme, but there is evidence that activation of protease-activated receptor-2 (PAR-2) leads to behavioral changes. There are no data on trypsin activity in the serum of animals under conditions of thirst and starvation in the available literature. In our experiments, water deprivation led to a significant (p⩽0.05) increase in trypsin activity in rats, and food deprivation led to its decrease in comparison with controls (free access to water and food). After deprived rats received water and food, a decrease in trypsin activity was observed in both experimental groups. Changes in trypsin activity under conditions of water or food deprivation and after satiation were accompanied by shifts in some biochemical parameters of the bloods. Under conditions of metabolic stress (starvation and thirst), opposite changes in trypsin activity seem to indicate its participation in the mechanisms of adequate restructuring of metabolism and maintenance of vital processes in the body.

[Evidence-Based Information Which Could Influence Arterial Hypertension Treatment Approach after Publication of SPRINT Trial Results].

The article discusses results of secondary analysis of the data obtained in the SPRINT study and published in recent years. Unresolved issues in the tactics of managing patients with arterial hypertension are discussed. One of such issues is choosing an optimum level of blood pressure (BP) for a subgroup of patients with certain characteristics, including elderly and senile patients, patients with chronic kidney disease, and patients with arterial hypertension who continue smoking. The article discusses calculation of a threshold of risk for complications of cardiovascular diseases, at which a maximum advantage of intensified regimens of antihypertensive therapy could be achieved. In addition, the article addresses approaches to selection of antihypertensive drugs in the current conditions. The authors discussed the role of candesartan in the treatment of arterial hypertension, a sartan most studied in a broad range of patients. The issue of a rapid increase in BP without a damage to target organs is addressed; evidence for the role of captopril in such clinical situation is provided.

[Consensus resolution on a modern view on the therapeutic and prophylactic effect of individual oral care products containing fluoride]. / Rezoliutsiia Ékspertnogo soveta 'Sovremennyi vzgliad na lechebno-profilakticheskoe deistvie individual'nykh sredstv dlia ukhoda za polost'iu rta, soderzhashchikh ftoridy'.

The purpose of the publication was to present the materials of the Expert Council Consensus on 'A Modern View on the Treatment and Preventive Effect of Fluoride Containing Personal Oral Care Products', which took place on April 21, 2019 in Moscow (Russia) with the support of the pharmaceutical company JSC GlaskoSmitKlein Helsker. During the work of the expert council, a solution was developed for the safe use of fluoride-containing oral hygiene products as part of fluoride prevention of dental caries. In the real conditions of everyday life, it is advisable for dentists to: use local fluoride-containing agents to know the concentration of fluoride in drinking water and in areas with low and optimal levels of it; recommend fluorides for daily use in small concentrations, for example, for children from 2 years of age to brush their teeth twice a day with fluoride toothpaste for at least 3 minutes; children up to 6 years old, when brushing their teeth once, use no more than a pea-size piece of toothpaste with 500 ppm of fluoride; toothpastes with fluoride concentration from 500 to 5000 ppm should be admitted depending on the risk level of caries development; rinses with a fluoride concentration of 0.025-0.05% should be recommended for children from 6 years and adults twice a day for 1 minute after brushing teeth; for patients with a high risk of caries, periodically (from 2 to 6 times a year) to carry out applications with agents with high concentration of fluorides; for prolonging the effect of local fluorine-containing agents advise patients to refrain from eating and drinking for an hour after their use. Dentists should know the methods of fluoride use in dentistry, since fluoride prevention of caries should be an integral part of their therapeutic and preventive work.

Key Drawbacks of Secondary Ischemic Heart Disease Prevention With Drug Therapy: the Role of Combination Fixed Dose Preparations in Solution of This Problem.

In this article we deal with the problem of low adherence of patients with ischemic heart disease (IHD) to drug therapy congruent to current recommendations including patients who underwent invasive interventions aimed at myocardial revascularization. Based on analysis of literature, we undertake an attempt to answer the question: which of components of this therapy patients with IHD are the least likely to continue taking? We also discuss approaches to the search for optimal composition of a combination preparation with fixed doses of an adreno-blocker and angiotensin converting enzyme inhibitor. Based on analysis of literature we undertook an attempt to answer a question: which of components of this therapy patients with IHD are least likely to continue taking. We also included in this article discussion of approaches to the search of optimal composition of a combination preparation containing fixed doses of a в-adrenoblocker and angiotensin converting enzyme inhibitor, as well as data on the role in the treatment of patients with IHD of bisoprolol and perindopril. Fixed doses of these agents have been included into a novel combination preparation.

[Current ideas of a complex relationship between atrial fibrillation and ischemic heart disease and of optimum tactics for anticoagulant therapy to prevent embolism in systemic circulation].

The article discusses the issue of searching for optimum oral anticoagulants to prevent thrombosis and embolism induced by heart disease both in patients with atrial fibrillation and sinus rhythm. A complex bidirectional relationship between atrial fibrillation and coronary atherosclerosis is considered along with possible mechanisms for development of myocardial infarction in patients with atrial fibrillation. The authors provided evidence-based data which can be used in selecting an anticoagulant for prevention of heart disease induced thrombosis and embolism taking into account both the efficacy and safety established in randomized clinical studies.

Interphase chromosome locus displacement induced by low-doses of radiation.

The most important stage in the making of mutations is a reparation of different DNA damage, including the more deleterious double-strand DNA breaks (DSB). The first stage of adaptive response--fundamental antimutagenic cell reaction, purposeful to reparation for induced DSB repair--is investigated in present work. Non-radioactive in situ hybridization of biotin-labeled DNA probe was used to mark chromosome 1 pericentromeric regions (PR) in G0 human lymphocytes. It was shown that under 3-10 cGy (X-radiation, 160 kV) PR become displaced from a nucleus periphery to inner territory of a nucleus. The moving process realizes during several hours after an irradiation. As far as some non-specific gene repressors are co-localized with chromosome centromeric regions it is possible hypothesizes that the displacement cause changing expression of some genes. It is possible to propose that an absence of radiation induced chromosome locus displacement may be one of causes DSB repair disturbance. This hypothesis was tested by the model. It is assumed that one consequence of the underlying defect may be inappropriate involvement of cell's recombination machinery in the repair of DSB. We studied lymphocytes of patients with hereditary BRCA2 mutation. It is thought that this gene takes part in DSB repair. The significant differences of the PR moving between control samples and the cases were revealed under 10 cGy. Similar results were observed on lymphocytes of patients with Fanconi syndrome. Thus, abnormal moving of interphase nucleus chromosomes conditioned by low-dose irradiation may suggest on imperfect machinery of DSB repair, i.e. genetic risk. We realize that further investigations are needed for definitive conclusion.

Two and a half-year outcome of caries-preventive programs offered to groups of children in the Solntsevsky district of Moscow.

This study examined the 2.5-year outcome of preventive programs - based on the Nexö method - offered to three groups of children from Solntsevsky, a district of Moscow. Study group A consisted of 45 3-year-olds, study group B of 50 6-year-olds, and study group C of 50 11-year-olds. A similar number of children were selected as control groups and they followed the normal dental service provided by the local Health Service System in the district. The caries-preventive programs offered to the study groups were based on: (1) education of the child, parents and teachers in the caries disease, (2) training in toothbrushing. In addition, the children in study groups B and C were offered professional plaque removal, applications of sodium fluoride (2%) and sealant applications according to individual needs. The children in groups B and C were clinically examined in March 1994 (baseline) after 1 and 2.5 years, respectively. Because of the age of the children in group A, these children were only examined once, after the study had been completed. After 2.5 years the children in all three study groups had improved their oral health status significantly compared to the children in the control group. The caries experience among the children in study group A was about half of that observed among children in the control group (4.91 def-s versus 8.60 def-s). The program was highly effective in controlling dental caries in the permanent dentition among the children in the study groups, who finished with a mean DMF-S of 0.28 (group B) and 3.12 (group C) compared to 2.24 and 6.35 among the children in the corresponding control groups. Nearly all the children used fluoridated toothpaste. The mean number of visits to the clinic decreased from year 1 to year 2 (5 versus 3.4 in study group B and 4.5 versus 3.3 in study group C). In conclusion, the preventive programs were highly effective with regard to improving the level of oral hygiene, and thereby reducing or even controlling the plaque-induced disease activity.

Relationship between external and histologic features of progressive stages of caries in the occlusal fossa.

The material comprised 140 extracted maxillary third molars. The central fossa area was examined with a stereomicroscope (SM) (x16) and macroscopically (M) under standardized conditions after cleaning and air-drying. Signs of caries were classified using a detailed scoring system involving 12 (SM) and 8 (M) classification criteria, ranging from 'sound' to 'cavitation with dentine involvement'. Six radiographic scores were used in the classification. Sections 250 microns in thickness were cut in buccolingual direction through the central fossa, and the fossa section with the most extensive stereomicroscopic changes was selected for histologic examination (x16). The histologic enamel and dentine changes were classified independently using 9 and 7 scores, respectively. The correlation between SM and the histologic enamel changes (HE scores) in terms of progressive demineralization and destruction were highly correlated (rs = 0.90). Dentinal changes were also highly correlated with enamel changes (rs = 0.85). The histologic classifications in conjunction with the macroscopical observations made it possible to demonstrate a clear relationship between the external degree of caries progression and the internal enamel and dentine reactions. The data did not support routine usage of radiographic examination for occlusal caries diagnosis.