[Computed tomography for assessment of postoperative outcomes in patients with parastomal hernia]. / Primenenie mul'tispiral'noi komp'yuternoi tomografii dlya otsenki effektivnosti khirurgicheskogo lecheniya patsientov s parakolostomicheskoi gryzhei.

OBJECTIVE: To show the efficacy of innovative hybrid intraperitoneal alloplasty in patients with parastomal hernia. MATERIAL AND METHODS: Treatment outcomes were assessed in 60 patients with parastomal hernia using computed tomography. CONCLUSION: The innovative hybrid intraperitoneal alloplasty reduces the risk of recurrence by 7 times (p=0.01).

[Evaluation of dentists regulatory systems stress during the provision of dental care according to pulse oximetry data]. / Otsenka napryazheniya regulyatornykh sistem vrachei-stomatologov v period okazaniya stomatologicheskoi pomoshchi naseleniyu po dannym pul'soksimetrii i saturatsii krovi.

OBJECTIVE: To evaluate the status of individual hemodynamic parameters (HR) and respiratory indicators of dentists performing local anesthesia. Material and methods. In the period from April 2019 to December 2019 the determination of heart rate (Heart rate) and blood saturation at 120 doctors aged 25-55 years was performed. The value of blood saturation by non-invasive pulse oximeter technique: SpO2 measurement range 0-99%; heart rate range 18-300 beats per minute. The limit of 95-98% was considered normal. However, we consider it correct to note the introduction of an error of 0.1% due to the constant wearing of a dentist's tight polymer mask. All subjects were preliminarily determined by Robergs-Landvere formula: maximum heart rate (MF)=205.8-(0.685·age). RESULTS: Maximum HR limits for the groups of subjects were 185.6±3.1 beats per minute in the first group; 178.7±3.1 beats per minute in the second group; 171.5±3.4 beats per minute in the third group. In all groups the tendency to decrease blood saturation in case of pain appearance in patients during treatment against the background of block anesthesia, as well as during anesthesia on the mandible was determined. The definition of SpO2 showed a slight downward trend in blood saturation when pain occurs within normal parameters. Thus, the limit of changes in the index against the background of performed local anesthesia on the mandible was 95.2-96.1% and 96.3-96.6% on the maxilla. CONCLUSION: Measurement of arterial oxygen saturation in dentists showed a tendency for a slight decrease in this indicator within the normal range when the patient developed pain. Thus, the limit of changes in the indicator against the background of local anesthesia on the lower jaw was 95.2-96.1%, on the upper jaw - 96.3-96.6%.

Nanoscale Generation of White Light for Ultrabroadband Nanospectroscopy.

Achieving efficient localization of white light at the nanoscale is a major challenge due to the diffraction limit, and nanoscale emitters generating light with a broadband spectrum require complicated engineering. Here we suggest a simple, yet highly efficient, nanoscale white-light source based on a hybrid Si/Au nanoparticle with ultrabroadband (1.3-3.4 eV) spectral characteristics. We incorporate this novel source into a scanning-probe microscope and observe broadband spectrum of photoluminescence that allows fast mapping of local optical response of advanced nanophotonic structures with submicron resolution, thus realizing ultrabroadband near-field nanospectroscopy.

Laser fabrication of 2D and 3D metal nanoparticle structures and arrays.

A novel method for fabrication of 2D and 3D metal nanoparticle structures and arrays is proposed. This technique is based on laser-induced transfer of molten metal nanodroplets from thin metal films. Metal nanoparticles are produced by solidification of these nanodroplets. The size of the transferred nanoparticles can be controllably changed in the range from 180 nm to 1500 nm. Several examples of complex 2D and 3D microstructures generated form gold nanoparticles are demonstrated.

[Design of experimental approaches on the base of standard proteins for testing blood biopreparations and immunoperoxidase conjugates specific to human and animal immunoglobulines G].

Using standard forms of immunoglobulin (Ig) G and albumin, we have studied electrophoretic and chromatographic profiles of samples of pharmaceutical blood biopreparation batches. The usability of standard proteins was also demonstrated by testing analytical characteristics of immunoperoxidase conjugates specific to human and animal IgG (anti-IgG IPC). In particular, we suggest an additional estimation of analytical characteristics of anti-IgG IPC by the enzyme reaction kinetics with the standard dilution which is calculated by the direct enzyme-liked immunoassay on the homologous IgG-antigen.

Laser-induced photopatterning of organic-inorganic TiO2-based hybrid materials with tunable interfacial electron transfer.

Hybrid organic-inorganic materials based on TiO(2) gels demonstrate high photosensitivity. Associated with their stable photochromic behavior, these make them suitable for laser-induced photopatterning. We show that the electronic coupling along the extended interface between the inorganic, TiO(2)-based gel, and the organic, poly(hydroxyethyl methacrylate) networks allows (i) a rapid scavenging of the photo-excited holes by the polymer, (ii) an efficient trapping of the photo-exited electrons as small polarons (Ti(3+)) that develop "dark" absorption continuum covering the spectral range from 350 nm (UV) to 2.5 microm (IR), and (iii) long-term (over months) conservation of trapped charges at high number density. Furthermore, we give the proof that the electron transfer depends on the material microstructure, which can be affected by the material chemistry and processing. Undeniably, a delay between the gelation of the system and the organic polymerization step allows tuning the photochromic responses of the resulting nanocomposites. A comparison is made between the prepared gel-based samples and a reference sample, which is obtained by the organic copolymerization of functional precondensed inorganic building units, titanium oxo-clusters, Ti(16)O(16)(OEt)(24)(OEMA)(8) with hydroxyethyl methacrylate. The experiments show the highest values of quantum yield (12%) and Ti(3+) concentration (1.7 x 10(20) cm(-3) or 14% of titanium atoms) attained in samples where the organic polymerization is induced after gelation. This behavior is explained by a strong coupling between the organic and the inorganic components of the hybrid towards the hole exchange and a poor coupling towards the electron exchange.

Laser-induced backward transfer of gold nanodroplets.

It is demonstrated that femtosecond laser-induced processes can be applied for controllable transfer of spherical gold nanodroplets from a thin gold film towards a glass substrate. The size of the transferred droplets depends on the volume of laser-molten gold and can be varied by changing the laser beam focus on the sample surface and the film thickness. Single- and multi-pulse fabrication of microstructures consisting of spherical gold nanodroplets is demonstrated. Mechanisms of these laser-induced backward transfer processes are discussed.

Supercontinuum assisted trapped electron accumulation in titanium oxide gel by femtosecond laser pulses.

Long-lived trapped electrons are generated in titanium oxide gels in a convergent part of a loosely focused beam from a millijoule, femtosecond Ti:Sapphire laser. The appearance of trapped electrons is accompanied by the generation of the white supercontinuum. Considering the relevant physical mechanisms of trapped electron accumulation, we show that two-photon interband transitions with participation of photons at the fundamental frequency and from the blue part of the supercontinuum explain the experimental findings.

Chemical activity of photoinduced Ti3+ centers in titanium oxide gels.

We report on the chemical activity of trapped electrons in wet titanium oxide gels. These electrons are generated under the band gap irradiation of gels in the spectral range between 3.25 and 4.4 eV and stored as Ti3+ centers that absorb in the visible. Chemical processes in photoirradiated gels are generally similar to those earlier reported in TiO2 colloids; however, peculiarities exist. In particular, a high internal surface of gels strongly enhances interface reactions. Measurements of UV-visible absorption kinetics allow conclusions to be made about extremely high available traps concentration and the activity of all trapped electrons toward nitrate and nitrite anion reduction according to a heterogeneous photocatalytic mechanism.

Light-induced charge separation and storage in titanium oxide gels.

We report on the interaction of light with a particular class of media--wet gels, which in contrast to sols of nanoparticles possess a macroscopic bulk structure, and which differ from conventional solids by the existence of the internal liquid-solid interface. We show, taking an absorption cross section of trapped electrons from Safrany, Gao, and Rabani [J. Phys. Chem. B 104, 5848 (2000)], that a separation of charges with quantum efficiency as high as 46% appears under the band-gap irradiation of titanium oxide gels: electrons are stored in the gel network as Ti3+ centers, whereas holes are stored in the liquid phase. Under a prolonged UV-laser irradiation, more than 14% of Ti4+ centers can be converted into Ti3+ . Their lifetime can be extremely long and exceeds months at room temperature. The trapped electrons are responsible for a "dark" absorption continuum covering the spectral range from 350 nm to 2.5 mum .

[Role of sialic acids in the immune response formation in tick-borne encephalitis]. / Rol' sialovykh kislot v formirovanii immunnogo otveta pri kleshchevom éntsefaliti.

The content of individual forms of sialic acids and total sialic acids in the lymphocytes of tick-borne encephalitis patients has been studied. The level of sialic acids has been found to depend on the clinical form of the disease and on the content of specific IgM and IgG to tick-borne encephalitis virus. Similar dependence has been established with respect to total sialic acids in lymphocytes.

[Organizational problems of the production of biological preparations of blood]. / Organizatsionnye problemy proizvodstva biopreparatov krovi.

The trend of the incidence of tick-borne encephalitis makes one pay attention to the production of blood biological preparations. A detailed programme for improving the production process, which is included into the federal and republican programmes, is outlined.

[The assessment of a state of tension in a pilot by bloodless biochemical and immunological study methods]. / Otsenka sostoianiia napriazhennosti letchika s pomoshch'iu beskrovnykh biokhimicheskikh i immunologicheskikh metodov issledovaniia.

Immunological and biochemical study of salivary liquid was carried out in order to estimate the tensity state of a pilot in operating conditions. These studies were conducted in the period of flight-development tests during man-compartible estimation of the cockpit of a light aircraft. The existence of natrium and secretory "A"-immunoglobulin in saliva in combination with psychophysiological parameters of tensity made it possible to reveal different levels of tensity proliferation (slight, moderate, strong and very strong). In the process of flight tests a correlative dependency was found between biochemical/immunological indices of salivary liquid, results of psychological testing, and informational/navigative loads of a pilot. This dependency was used during laboratory testings which were included in the complex of researches on man-compartible characteristics in the system "pilot-cockpit-aircraft".

Mechanisms of thrombocytopenia in patients with lymphoproliferative diseases.

Different factors are involved in the development of thrombocytopenia in patients with lymphoproliferative disorders. Significant correlation was detected between the number of megakaryocytes in bone marrow and platelet count (r = 0.485, p = 0.002, n = 37) and significant difference between the number of megakaryocyte in patients with normal platelet count (> 200,000/microliters) and patients with marked thrombocytopenia (platelet count < 100,000/microliters). All patients in the latter group (n = 15) had a relatively low number of megakaryocytes. Low but significant reverse correlation was found between the level of platelet-associated IgG (PA-IgG) and platelet count (r = -0.249, p = 0.024, n = 82) and significant difference between the mean levels of PA-IgG in the groups of patients with platelet count > 200,000/microliters and < 100,000/microliters. PA-IgG were increased in 46% of patients in the total group and in 65% of patients with platelet count < 100,000/microliters. The correlation between platelet count and PA-IgG was about 2 times higher in splenectomized (r = -0.549, p = 0.005, n = 24) than nonsplenectomized patients. All splenectomized patients with platelet count < 100,000/microliters (n = 8) had a significant increase in PA-IgG. Serum antibodies were detected in only 7% of tested patients. This group was characterized by severe thrombocytopenia (in 6 of 10 patients--platelet count < 50,000/microliters) and a high incidence of haemorrhages (in 5 of 10 patients). Thus the depression of platelet production was suggested to be the basic cause of thrombocytopenia in lymphoproliferative disorders. Involvement of immune mechanisms was revealed in a large number of patients and correlated with a deeper and more complicated thrombocytopenia.

[The use of hemoperfusion through a suspension of cryopreserved hepatocytes in acute liver failure]. / Primenenie gemoperfuzii cherez vzves' kriokonservirovannykh gepatotsitov pri ostroi pechenochnoi nedostatochnosti.

The present work deals with results of intensive therapy and temporary organ substitution by using hemoperfusion through a suspension of living donor hepatocytes in 71 patients with acute hepatic insufficiency. Hemoperfusion was performed through a suspension of fresh living hepatocytes or through a suspension of cryo-conserved (during 60 days) living hepatocytes. Lethality was 37-42%.

[Determination of antiplatelet antibodies on the surface of platelets by direct radioimmune method in patients with different types of immune thrombocytopenia]. / Opredelenie antitrombotsitarnykh antitel na poverkhnosti trombotsitov bol'nykh s razlichnymi formami immunnoi trombotsitopenii priamym radioimmunnym metodom.

Direct radioimmune assay (RIA) have been developed for detection of antibodies associated wild platelet membrane. Platelets from 12 patients with idiopathic thrombocytopenic purpura (ITP) and 27 patients with chronic lymphocytic leukemia (CLL) (platelet count (100,000 in 1 microliters) have been tested. Antibodies on platelets surface have been detected in all 12 patients with ITP and in 21 patients with CLL. In 6 CLL patients the number of immunoglobulins associated with platelets surface does not increase control level. It is possible, that in some CLL patients development of thrombocytopenia is mediated not only by platelet associated antibodies but by other mechanisms, one of which can be linked with the depression of megakaryocytes growth in bone marrow. Direct RIA for measurement of antibodies on platelet surface detect antiplatelet antibodies with higher frequency than indirect enzyme-linked-immunosorbent-assay (ELISA), developed earlier for assessment of antiplatelet antibodies in serum. Increase of platelet count in CLL patients after steroid and cytostatic treatment correlated with the decrease of platelet surface associated antibodies.