ABSTRACT
The functional activity of bcl-2 andp53 proteins in erythrokariocytes of erythroblastic islands (EI) of various classes of bone marrow belonging to intact rats and animals following an acute blood loss were investigated. It is observed that intact rats had the elevated levels of percent content of bcl-2+ erythrokariocytes in EI of the second maturity class, compared to EI of first maturity class and EI involutionary class, and also less in the EI involutionary class compared to EI 1 and EI 2. The percentage content of p53+ erythrokariocytes in intact rats in the EI 2 class was less than in EI 1 and EI involutionary class, and were nearly the same of EI 1, EI 3, EI involutionary class and EI reconstructed. 48 hours after blood loss, the content of bcl-2+ erythrokariocytes were increased in EI 1, EI 2, and EI reconstructed.
Subject(s)
Apoptosis , Bone Marrow/metabolism , Erythroblasts/metabolism , Hemorrhage/metabolism , Proto-Oncogene Proteins c-bcl-2/metabolism , Tumor Suppressor Protein p53/metabolism , Animals , Bone Marrow/pathology , Erythroblasts/pathology , Hemorrhage/pathology , Male , RatsABSTRACT
The modification of peritoneal cells derivation method proposed for the peritoneum rat, and the absolute amount of peritoneal lymphocytes, monocytes, macrophages, stem and polymorphonuclear neutrophyles, mat cells was determined. The procedure of acute 2% blood loss was found to reduce the total amount of the peritoneal cells due to depletion of lymphocytes accompanied by an increase in amount and proliferative activity of monocyte peritoneal cells, stem neutrophiles appearance in the peritoneal cavity. The subcutaneous injection of colchicine solution reduces the number of the peritoneal macrophages in normal rats, decreases the number of the monocytes, polymorphonuclear neutrophiles and led to disappearance of the stem neutrophiles on the second day of blood loss.
Subject(s)
Hemorrhage/physiopathology , Leukocytes/cytology , Macrophages, Peritoneal/cytology , Peritoneum/physiopathology , Animals , Cell Count , Cell Separation/methods , Colchicine/administration & dosage , Colchicine/pharmacology , Injections , Leukocytes/drug effects , Macrophages, Peritoneal/drug effects , Male , Peritoneum/drug effects , RatsABSTRACT
Erythroblastic islands of the bone marrow are morpho-fuctional units of erythropoiesis. The functional state of the erythroblastic islands' erythrokariocytes and central macrophages of the bone marrow was first studied by estimation of the content of ribonucleoproteids in its cells (Brashe reaction). Acute blood loss and posttransfusion polycytemia lead to enhancement of the content of ribonucleoproteids in erythrokariocytes and central macrophages of the bone marrow of the rates.
Subject(s)
Bone Marrow/metabolism , Erythroblasts/metabolism , Erythropoiesis , Macrophages/metabolism , Polycythemia/metabolism , Ribonucleoproteins/metabolism , Animals , Hemorrhage/metabolism , Male , RatsABSTRACT
Erythroblastic islands (EIs) of the bone marrow are the morpho-functional units of the erythron. The functional state of the cells of EIs of varying maturity was first studied by estimating the activity of nucleolar organizers in intact rats and in rats with acute blood loss or posttransfusion polycythemia by the modified method of J. Croker and P. Nar. The intact rats had the largest number of nucleoli and intranuclear argentum inclusions located within and outside the nucleoli in EIs of proliferating classes: Class 1 EIs and reconstructing EIs and the smallest number in involutional EIs. Blood loss was accompanied by the enhanced activity of nucleolar organizers in EI cells. In polycythemia, the activity of the organizers changed in a more complicated manner.
