[DIETARY SUPPLEMENT REKECEN IMPROVES SAFETY AND EFFICACY OF CHRONIC PROSTATITIS ANTIBACTERIAL THERAPY].

This randomized comparative study was carried out to estimate efficacy and safety of the natural complex of fermented food fibers and short chain fatty acids (dietary supplement rekicen-RD®) in antibacterial therapy of 64 patients with chronic infectious prostatitis. 32 patients of the treatment group received 8 week standard antibacterial therapy for chronic prostatitis in combination with dietary supplement rekicen-RD® and 32 patients of the control group were treated only with antibacterial therapy. Short-term and long-term results were estimated after 8 weeks and 6 months follow-up, respectively. It was found, that addition of dietary supplement rekicen- RD® to antibacterial therapy resulted in statistically significant improvement of the treatment efficacy. Compared to patients of the control group, patients of the treatment group had more pronounced positive changes of all indicators of treatment efficacy (NIH-CPSI total score, quality of life, echo-structure of prostate, the number of leukocytes in prostatic secretions). Long-term (after 6 months) clinical efficacy of the combination of antibacterial therapy with dietary supplement rekicen-RD® was 96,9%, bacteriological efficacy after 8 weeks - 87,5%, after 6 months - 81%. Notably, there was 4,5-fold reduction in the rate of antibiotics adverse side effects in the treatment group patients without a single gastro-intestinal side effect.

[Sensitization of lymphocytes to insulin as one of risk factors of ischemic heart disease].

Prevalence and character of sensitization of lymphocytes to insulin and its prognostic significance were studied in 71 patients with ischemic heart disease and 55 healthy people. Direct overt sensitization of lymphocytes to insulin was found in 14 of 71 patients (p<0.01 compared with control group). This type of reaction was associated with myocardial infarction. Concealed indirect reaction of lymphocytes to insulin was revealed at inhibition of cells with receptors to histamine or prostaglandin synthesizing cells in 13 and 19 of 71 patients, respectively (p<0.01 and p<0.05 compared with control group). Direct overt sensitization of lymphocytes to insulin and concealed indirect sensitization revealed at inhibition of functional activity of cells with receptors to histamine serve as prognostically unfavorable factors because they are associated with development of heart failure and higher rate of lethal outcome.

[A comparative evaluation of different immunological reactions in the diagnosis of pseudotuberculosis]. / Sravnitel'naia otsenka razlichnykh immunologicheskikh reaktsii v diagnostike psevdotuberkuleza.

To study humoral response in patients with the generalized form of pseudotuberculosis, the agglutination test, the passive hemagglutination test and the complement fixation test with antigen obtained from "Virion" (Switzerland) were used. The study of the dynamics of antibody formation to outer membrane proteins (OMP) and Yersinia pseudotuberculosis (pYV+ and pYV-) lipopolysaccharide (LPS) revealed that the synthesis of antibodies to OMP, controlled by inv gene, exceeded the synthesis of antibodies to LPS at all periods of the disease, which determined the necessity of further study of the role of OMP in pathogenesis and in the formation of humoral response. On the basis of the study of the occurrence of antigens a high diagnostic effectiveness the enzyme immunoassay for the detection of Y. pseudotuberculosis antigens, mainly at the first stage of the disease, was noted.

[Methodical bases of the use of erythrocytic diagnostic agents as phagocytosis objects]. / Metodicheskie osnovy primeneniia éritrotsitarnykh diagnostikumov v kachestve ob"ektov fagotsitoza.

The error in assessment of the neutrophil phagocytic activity is 5.8%. This is attained by removal of conglomerations of phagocytosis objects and by ruling out the minimal variations in the duration of contact between the blood and foreign corpuscles and preparation of smears to be examined on the same slide. The content of up to 90% red cells does not influence the neutrophil phagocytic activity, and higher concentration reduces its level.

[The phagocytic test in the assessment of antimicrobial protection in inflammatory urologic diseases]. / Fagotsitarnyi test v otsenke antimikrobnoi zashchity pri vospalitel'nykh urologicheskikh zabolevaniiakh.

As shown in in vitro original modelling of red cell action on phagocytic process in the whole blood of patients with urological inflammation, these patients have increased count of neutrophils involved in phagocytosis, but low specific absorption of these neutrophils due to red cells. In chronic renal insufficiency characterized by a low phagocytic activity of neutrophils only stimulating potential of red cells can be noted.

[New methodological approaches to the use of the PHA reaction in determining anti-Shigella antibodies]. / Novye metodicheskie podkhody k ispol'zovaniiu RPGA pri opredelenii protivoshigelleznykh antitel.

Pronounced correlation between the titers of hemagglutinating antibodies to different types of shigellae and other bacteria in healthy persons and in sick persons of control groups has been established. On the basis of this regularity a new approach to the use of the passive hemagglutination test in shigellosis, taking into account the ratio of antibody titers to different Shigella types, has been developed. This essentially increases the sensitivity and specificity of the test, thus making it possible to standardize its evaluation and permitting the effective diagnosis of shigellosis from the onset of the disease.

[Intestinal dysbacteriosis in yersiniosis patients and the possibility of its correction with biopreparations]. / Disbakterioz kishechnika u bol'nykh iersiniozami i vozmozhnost' ego korrektsii biopreparatami.

Intestinal dysbacteriosis was found in all 37 patients with intestinal yersiniosis or pseudotuberculosis. Administration of biopreparations containing lacto- or bifidobacteria produced a good clinico-microbiological response rate (above 90%). The authors think it valid to include biopreparations in combined treatment of yersiniosis to prevent unfavourable outcomes.

[The functional activity of the peripheral blood neutrophils in patients with chronic brucellosis]. / Funktsional'naia aktivnost' neitrofilov perifericheskoi krovi u bol'nykh khronicheskim brutsellezom.

The functional activity of peripheral blood neutrophils was studied by their main functional and metabolic parameters. The group of chronic brucellosis patients under study included 32 patients. The control group consisted of 35 practically healthy donors. The complex of methods, used for the evaluation of the functional activity of peripheral blood neutrophil leukocytes, included the determination of adhesion, activity of nitro blue tetrazolium reduction, enzymatic activity of myeloperoxidase and acid phosphatase, the level of cation protein and the production of active forms of oxygen (AFO) by these cells. Chronic brucellosis patients were found to have disturbances in the adhesive capacity of phagocytizing cells and the production of highly bactericidal AFO. These disturbances in the activity of phagocytizing cells may be one of the mechanisms contributing to incomplete phagocytosis in chronic brucellosis in humans.

[Indicators of oxygen-dependent metabolism of peripheral blood neutrophils in the differential diagnosis of bacterial and viral infections]. / Pokazateli kislorodzavisimogo metabolizma neitrofilov perifericheskoi krovi dlia differentsial'noi diagnostiki bakterial'noi i virusnoi infektsii.

Oxygen-dependent metabolism of peripheral neutrophils was compared for viral hepatitis and acute yersiniosis patients. The metabolism inhibition was seen in both the diseases with lower values for viral hepatitis. Neutrophil function was dependent on the disease gravity. Its recovery went in parallel with other clinical and laboratory signs of convalescence. A luminol-dependent chemiluminescence test is proposed for differential diagnosis of viral hepatitis with jaundices in generalized bacterial infection.

[Antigen isolation from the blood of patients with malaria for its serological diagnosis]. / Poluchenie antigena iz krovi bol'nykh maliariei dlia ee serologicheskoi diagnostiki.

A relatively high sensitivity and specificity of indirect enzyme immunoassay using the antibody obtained from the blood of patients with P. falciparum-induced malaria in the acute period have been demonstrated. The substrate for antigen isolation is available and can be easily obtained in sufficient quantities.

[Clinical picture of tropical malaria in persons having undergone chemoprophylaxis]. / Klinika tropicheskoi maliarii u lits, provodivshikh khimioprofilaktiku.

Suppressive therapy with chloroquine and fansidar at standard doses does not prevent malaria at the time of a maximum level of infecting agent transmission but only weakens the expression of clinical manifestations. The main clinical signs are nonspecific symptoms of infectious intoxication. At the seasonal peak of tropical malaria incidence, doses of individual chemoprevention should be doubled as compared to the standard ones.

[Pneumoencephalography in multiple sclerosis]. / Pnevmoéntsefalografiia pri rasseiannom skleroze.

Pneumoencephalography was performed in 35 patients with a significant diagnosis of disseminated sclerosis. Enlargement of the III ventricle was revealed in 21 patients (60%), dropsy of lateral ventricles in 16 (45,6%) and that of the IV ventricle in 13 (37,2%), atrophy of brain stem in 9 (25,4%), block of the subarachnoidal space in 11 patients (31,4%). The most pronounced brain changes were seen in patients with a cerebellar form of disseminated sclerosis and in those having severe motor disturbances, ataxia and tremor. The degree of hydrocephalus did not depend on the disease duration. A possible pathogenetic role of dropsy in disseminated sclerosis is discussed. Hydrocephalus may be considered as an additional clinicoroentgenological feature of disseminated sclerosis.