ABSTRACT
Plasmic, platelet and fibrinolytic components of hemostasis were studied in 115 patients with severe viral hepatitis C transmitted fecally and orally. Most informative for determination of the disease severity and prognosis of onset of acute hepatic encephalopathy within 1-2 days were plasmic factor II, V, VII, X. In genesis of hemorrhagic syndrome of importance is procoagulant deficiency as a result of detective synthesis and consumption due to DIC syndrome rather than platelet disorder. In addition to procoagulant disorders there were low levels of plasminogen and its inhibitors changing with the disease severity.
Subject(s)
Blood Coagulation , Hemorrhage/etiology , Hepatitis C/blood , Acute Disease , Adolescent , Adult , Female , Hemorrhage/blood , Hepatic Encephalopathy/etiology , Hepatitis C/complications , Humans , Male , Middle Aged , Severity of Illness Index , SyndromeABSTRACT
A decrease in the capacity of lymphocytes for rosette formation, accompanied by a rise in the leukocyte count, has been found in patients with the severe form of hepatitis non A, non B. Neutrophils in these patients have shown a tendency towards an increased rosette formation. All altered characteristics normalized by the period of convalescence. As noted in this investigation, a considerable increase in the leukocyte count, along with an increase in the number of active rosette-forming neutrophils and a decrease in the capacity of leukocytes for rosette formation, is an unfavorable sign predicting the possibility of acute hepatic encephalopathy.
Subject(s)
Feces/microbiology , Hepatitis C/immunology , Hepatitis, Viral, Human/immunology , Lymphocytes/immunology , Neutrophils/immunology , Adolescent , Adult , Female , Hepatic Encephalopathy/etiology , Hepatic Encephalopathy/immunology , Hepatitis C/complications , Hepatitis C/transmission , Humans , Leukocyte Count , Male , Pregnancy , Pregnancy Complications, Infectious/immunology , Pregnancy Complications, Infectious/transmission , Rosette FormationABSTRACT
We developed a host-vector system for transformation and gene cloning experiments using the methylotrophic yeast Hansenula polymorpha. Regeneration of protoplasts in a medium containing polyethylene glycol before plating made transformation more efficient and reproducible (2 to 3 x 10(4) micrograms DNA). The frequency of transformation was significantly lower when dominant resistance marker Cup1r was used for transformant selection. The transformation system developed was used to clone the DNA fragment which complements functionally the defect in the dihydroxyacetone kinase (DHAK*) activity of a H. polymorpha mutant strain. The DNA insert isolated was shown to increase by up to ten times the activity of DHAK in transformants carrying recombinant plasmids. When recombinant plasmids were introduced into S. cerevisiae, the transformants obtained acquired the ability to grow on the medium with dihydroxyacetone as a sole carbon source and the activity of DHAK was observed.
Subject(s)
Cloning, Molecular , Phosphotransferases (Alcohol Group Acceptor) , Pichia/genetics , Saccharomycetales/genetics , Transformation, Genetic , Escherichia coli/genetics , Genetic Engineering , Genetic Markers , Genetic Vectors , Methanol , Phosphotransferases/genetics , Pichia/enzymology , PlasmidsABSTRACT
The paper is concerned with an analysis of clinico-biochemical indices and 14 parameters of the plasmatic and platelet links of hemostasis in 29 patients with acute virus hepatitis of a varying degree of severity treated by hyperbaric oxygenation and in 40 controls. HBO made a modulating effect on most indices of hemocoagulation and fibrinolysis bringing them nearer to a certain standard which was optimum for a certain stage and degree of AVH. Probable mechanisms of the HBO effect on various indices of homeostasis in AVH and clinico-pathogenetic importance of the state of hemocoagulation against a background of HBO-therapy were discussed.
Subject(s)
Blood Coagulation , Hepatitis A/therapy , Hepatitis B/therapy , Hyperbaric Oxygenation , Acute Disease , Adolescent , Adult , Combined Modality Therapy , Convalescence , Evaluation Studies as Topic , Female , Hepatic Encephalopathy/blood , Hepatic Encephalopathy/etiology , Hepatic Encephalopathy/therapy , Hepatitis A/blood , Hepatitis A/complications , Hepatitis B/blood , Hepatitis B/complications , Humans , MaleABSTRACT
For the transformation of the yeast Hansenula polymorpha we have constructed a set of hybrid plasmids carrying the LEU2 gene of Saccharomyces cerevisiae as a selective marker and fragments of mitochondrial DNA of Candida utilis and H. polymorpha or chromosomal DNA fragments of H. polymorpha as replicator sequences. The replication properties of chimeric plasmids in the yeast H. polymorpha were investigated. We showed that for plasmids propagated autonomously in this yeast the plasmid monomers could be detected in the transformants only during the immediate time after the transformation event. Further growth under selective conditions led to the selection of polymeric forms of plasmid DNA as it was clearly shown for transformants carrying cosmid pL2 with mtDNA fragment of C. utilis. Such transformants carrying polymerized plasmids showed a remarkably increased stability of the transformed phenotype. Cosmid pL2 was able to shuttle between Escherichia coli, S. cerevisiae and H. polymorpha, whereas plasmids with DNA fragments from H. polymorpha did not transform S. cerevisiae effectively.
