[The influence of probiotic fermented milk product on colon microbiota, hematological parameters and cell immunity in rats].

Influence of probiotic fermented milk product on the intestinal microbiota, hematological parameters and immune status of the experiment in vivo at Wistar rats was studied. It was shown, that entering of probiotic strains of Bifidobacterium bifidum 791, Bifidobacterium longum B-379M and Lactobacillus acidophilus NK1 u Streptococcus thermophilus in composition fermented milk products in the total quantity of 2,1 x 10(7) CFU/ sm3 in digestive tract within three weeks has a positive influence on the resident of colon microbiota. Significant increasing of population levels of Bifidobacterium, Enterobacteriaceae with normal biochemical properties, registered a strong tendency to increase the content of Lactobacteria, which led to a decreasing the number of potential pathogenic transient flora with pathogenic factors. Monitoring of body mass in experimental animals has shown that including of fermented milk product with probiotic strains in diet has a positive influence on the feed uptake. Probiotic properties of the product also have stimulated effect on the immune status of the rat: improvements in cell immunity (increasing the relative amount of T-helper cells, immuneregulatory index) and hematological parameters (increase

[The influence assessment of probiotic fermented milk products on intestinal microflora, hematological indices and cellular immunity in rats (report 1)].

Influence of probiotic fermented milk products on the intestinal flora, hematological parameters and immune status of the experiment in vivo at rats is studied. Entering in digestive tract of probiotic strains of Lactobacillus acidophilus NK-1 and Bifidobacterium bifidum 791 at the level of hundreds of millions CFU in a day (from 1,7 x 10(8) to 9 x 10(8) CFU) in composition fermented milk products renders on the whole positive, but not significant statistically influence on the indexes of colon microflora and immune status of rats, and it must be extended for the achievement of reliable effect.

[Influence of lactobacillus strains used in the production of dairy products on the agents of food poisoning].

Study of antagonistic activity of 33 strains of Lactobacillus isolated from the industrial preparation of fermented milk products, starter cultures and biologically active additives and products of natural fermentation. Antagonistic activity was studied by agar diffusion method with holes. Strains isolated from products of natural fermentation showed high degree of antagonistic action together with industrial strains.

[Study composition of lactoflora of the large intestine in patients with food allergy and irritable bowel syndrome].

Patients of different ages with various forms of food allergy and also with the irritable bowel syndrome with locks are studied. The quantitative levels of bifido- and lactoflora of large intestine, the qualitative characteristics of lactoflora population, including specific gravity of individual representatives in the sum of the isolates, are investigated, species composition is identified. Shown that food allergy, irrespective of the disease manifestation form induces the balance disturbance of bifido- and lactoflora in large intestine, significantly affects the reduction in the number of species of Lactococcus and Leuconostoc spp. and on the narrowing of the enzymatically active species of Lactobacillus spp.

[Toxicological sanitary characterization of silver nanoparticles introduced in gastrointestinal tract of rats].

Water suspensions of silver nanoparticles were introduced intragastrically to growing male Wistar rats daily for 28 days. There were studied animals mass gain, relative masses of viscera, intestinal barrier resistance against protein macromolecules, DNA oxidative damage, tissue non-protein thiol levels, first and second phase of xenobiotic detoxication system activity, lysosomal membranes stability in liver together with routine blood biochemical and hematological indices and caecal's microbiocenose state. The data testifying to possible toxic risks, connected with reaction of silver's nanoparticles is obtained.

[Toxicological sanitary characterization of titanium dioxide nanoparticles introduced in gastrointestinal tract of rats. Communication 2. Intestinal microbiocenosis condition and allergic sensitivity].

Intestinal microbiocenosis condition, cytokine IL-6, IL-10 and TNF-alpha levels and allergic sensitivity in experimental systemic anaphylaxis test were studied in rats that received intragastrically nanoparticles of titanium dioxide. The results obtained were discussed from the standpoint of possible health risks related to nanodimensional titanium dioxide exposure.

[Effect of carrageenan on indices of protein biological value and assimilability].

Influence of different levels of dietary carrageenan (0.2; 0.6, 2% with 18% casein) after 4 weeks in rats on coefficients of biological value (PER, NPR, NPUtr u BVtr) and digestibility (Dtr) of protein, microflore in cecum and morphometric data in distal part of small intestine were studied. There were found adverse changes of coefficients of biological value (NPUtr, BVtr) and digestibility (Dtr) after 2% dose of carrageenan. Chandes in microflore of cecum and worse of morphometric data in small intestine were observed.

[An influence of fungi spores consumption with food on intestinal microflora in rats].

In experiments in rats it is set, that peroral introduction with the feed of mould spores in a doze 10(3) CFU/g results in a disbalance of microbiocenosis of intestine. It shows up violation of localization of bifido- and enterobacteria and their increased growth in a small intestine. Thus, also antagonistic activity of an aerobic component of microflora is reduced and frequency of detection of potential-pathogenic types of enterobacteriaceae is increased. Entering of fungi spores at a dose 10(6) CFU level is accompanied by more expressed infringements of aerobic populations of intestinal microflora in rats.

[Influence of the food products, enriched with pectin, on properties of potentially patogenic representatives of the microflora of the large intenstine].

The treatment method of disbios suffered by patients with syndrome of irritated intestines with constipations was under research. The method was based on introduction of sour-milk beverage with addition of beet pectin in a dietary intake. The comparison product was kefir. The following developments were detected against the positive dynamics of clinical symptoms: improvement of microbiocenosis structure, reduction of potentially pathogenic germs, inhibition of pathogenic properties of enterotoxigenic Staphylococcus aureus. Comparison group results showed lower clinical efficiency and poorer effect of dietary treatment on large gut microorganisms, contents of enterotoxigenic S. aureus remained unchanged. Introduction of sour-milk beverage with addition of alimentary fibre in a dietary intake of patients suffering the syndrome of irritated intestines with constipations was found expedient.

[Enterotoxin production as a diagnostic test in intestinal dysbacterioses ]. / Enterotoksinoobrazovanie kak diagnosticheskii test pri disbakterioze kishechnika.

The new approach to advancing diagnostic of dysbacterioses grounded on determination of the pathogenic factors of potential-pathogenic microflora is proposed. The method of reacting of an indirect hemagglutination with usage of erythrocital diagnosticums ostend availability of production of staphylococcal enterotoxines of types A and B in 40% of the strains of staphylococcuses, isolated at dysbacterioses. The intensity of production of staphylococcal enterotoxines for strains of microorganisms, isolated at dysbacterioses and carrying of S. aureus, and at food borne diseases, is various. The availability of clinical developments of an intestinal dysfunction in faeces of the patients contacts with persistence of enterotoxigenics S. aureus, and their elimination--with successful police of the disturbed microbiocenosis.

[Effect of erithriol on intestinal microflora and morphology of rat internal organs]. / Vliianie éritrita na mikrofloru kishechnika i morfologiiu vnutrennikh organov krys.

In experiences on rats is shown, that 22 daily upkeeping of animals on a diet including eritrit in a dose 1 g/weight of a body, do not cause any changes from the party intestinal microflora and morphological structure of internal bodies. However, the specified doze eritrit causes statistically authentic increase of levels conditionally-pathogenic anaerobius of the representatives microflora, first of all staphylococcus and citratessimilation enterobacteria.

[Comparative study of the effects of different sour milk products on the intestinal microflora of healthy infants]. / Sravnitel'naia otsenka vliianiia razlichnykh kislomolochnykh produktov na kishechnuiu mikrofloru u detei rannego vozrasta: neodnoznachnost' éffektov.

Our study was aimed to investigate in comparable conditions the composition of intestinal microflora of healthy infants who were given 9 types of fermented milk products. There were observed 103 practically healthy infants aged from 4 month to 1,5 years old. The obtained data have shown that studied products improve intestinal microflora in infants. Significant increase of bifidobacteria in infants intestines was promoted by fermented milk products containing alive bifidobacteria with rather high acidity. The most of products which contain bifidobacteria decreased the level of hemolisinproducing Escherichia coli and coagulase-positive staphylococcus. The products which not contain bifidobacteria decreased the level of Candida albicans and protei.

[Antagonism of microbial population of protective flora and its relation to microbiocenosis and nutritional factors]. / Antagonisticheskaia aktivnost mikrobnykh populiatsii zashchitnoi flory i eë sviazi s kharakteristikoi mikrobiotsenoza i faktorami pitaniia.

Total antagonistic activity (TAA) of the populations of bifidobacteria, lactobacilli, enterobacteria was considered in relation to the method of its evaluation which has been modified, criteria of TAA assessment permitting its measurements in per cents. The technique proposed bases on determination of the metabolites capable of inhibiting pathogenic and opportunistic bacteria and provides information on the action of alimentary factors under study on the function of intestinal microbiocenosis. The factors may produce a multidirectional effect on the structure and biological activity of the microbial populations. The method can work to characterize microbiocenosis both in normal and pathological conditions, to evaluate efficacy of specially devised food products.

[A micromethod for the rapid detection of proteolytic activity of microorganisms]. / Mikrometod dlia ékspressnogo vyiavleniia proteoliticheskoi aktivnosti mikroorganizmov.

The author has developed a micromodification of the rapid photoemulsion method for the detection of the microorganism proteolytic activity. The test is carried out on polystyrene plates, meat-peptone broth is used as the suspension fluid. Rectangular film strips are vertically placed into the wells, thus preventing false-negative reactions possible in case of an erroneous horizontal position of the film with the emulsion layer turned upwards if square or round film fragments are used. The proteolytic activities of 120 microorganism strains (95 of these hydrolyze gelatin) have been examined by the micromethod and the routine test with gelatin. The suggested test is 2-4-fold more rapid than the routine one used in investigations of the cultures slowly hydrolyzing gelatin. The fact that the test is carried out on the plates considerably reduces the nutrient medium and the number of laboratory glassware and helps obtain more accurate results.

[Biochemical properties of enterobacteria on polystyrene plates]. / Izuchenie biokhimicheskikh svoistv énterobakterii na polistirolovykh plastinakh.

The authors studied 84 strains of enterobacteria. The reliability of the results of the biochemical testing obtained by the micromethod with the use of polystyrene plates was examined comparatively to the data obtained by the classical method and API microsystems manufactured in France. Of the 84 strains of enterobacteria, 36 belonged to Proteus, 24 to Enterobacter, 8 to Klebsiella, 5 to Citrobacter, and 11 to E. coli. Of the strains tested, 50 were isolated from the intestine of children and adults, 30 from the residual microflora of foods, and 4 were museum strains. Study according to 23 tests on the conventional media with consideration of the results after 18 to 48 hours demonstrated the coincidence of the data, obtained by the methods used, for most strains. This circumstance permits recommending the micromethod for testing the biochemical properties of enterobacteria on polystyrene plates for broad-scale use.

[Effect of a derivative of GABA, phenibut, on behavior and the activity of visual cortex neurons of the rabbit during elaboration of a defensive reflex and internal inhibition]. / Vliianie proizvodnogo GAMK--fenibut na povedenie i aktivnost' neironov zritel'noi kory krolikov pri vyrabotke oboronitel'nogo refleksa i vnutrennego tormozheniia.

In experiments on alert non-immobilized rabbits the effect of subcutaneous administration of the GABA-derivate--phenibut on behaviour, slow potentials and impulse activity of neurones of the visual cortex was studied during elaboration of a defensive reflex to light flashes and of conditioned inhibition. During the action of phenibut late negative-positive components of the evoked potentials to flashes, corresponding inhibitory pauses and postinhibitory activation gradually increased; then stable predominance of slow high-amplitude potential oscillations and corresponding neuronal group bursts appeared, separated by inhibitory pauses and in intersignal periods. Reinforcing stimulus, as before phenibut administration, lowered the amplitudes of slow potential oscillations and weakened inhibitory pauses in neuronal impulse activity. Dynamics of movements in response to the stimuli was of a phasic character. 3 hours after phenibut administration the discrimination of reinforced and inhibitory light flashes has distinctly improved. The obtained results confirm the initial concept of the significant role of the GABA-ergic inhibitory system in the process of elaboration of internal inhibition.