[Retinoic acid modification of cell culture used for reproduction of enteropathogenic viruses]. / Modifikatsiia retinoevoi kislotoi kul'tury kletok, ispol'zuemoi dlia reproduktsii énteropatogennykh virusov.

The 0.001-0.005% retinoic acid injection into the growth cultural medium of prime and continue cell cultures 12-24h before inoculation considerably raised the cell sensitivity to animal entero- and coronaviruses. The entero- and coronaviruses concentrations in cultural medium increased by 10(1.58) and 10(1.68)TCID 50/1.0 respectively. The optimized parameters of the cell culture processing for the enteropathogenic viruses reproduction improvement are proposed.

[Thymus-dependent nature of the antigens of enteropathogenic viruses]. / Timus-zavisimaia priroda antigenov énteropatogennykh virusov.

The nature of antigens in enteropathogenic animal viruses--coronavirus--an agent of transmissive gastroenteritis of pigs (TGE) and pig enterovirus of serotype VI was studied. Basing on the results of the histomorphometric study of the white spleen pulp under the individual introduction of viruses and in combination with the lymphoid chalone it was established that the developing immune response has a thymus-dependent induction mechanism. The pharmacological stimulation of the T-system of animals suffering from virus gastroenteritis provides a positive therapeutic effect. The selective stimulation of the animal T-system may be considered as a promising trend in therapy of intestinal infections and as one of the possible ways to increase the immune response to the antigens of viral vaccines.

Acyclic analogs of nucleosides. Synthesis and in vitro antiviral activity of hydroxyalkyl-2-(trifluoromethylthiomethyl) benzimidazoles.

In a search for novel antiviral compounds of the 'doubly modified' nucleoside type, we have prepared 1-(4-hydroxy-2-oxabutyl)-, 1-(4-hydroxy-3-hydroxymethyl-2-oxabutyl)-, 1-(4-hydroxy-1-hydroxymethy1-2-oxabutyl)-, 1-(4-hydroxy-1-methyl-2-oxabutyl), 1-(4,5-dihydroxy-2-oxapentyl)-, 1-(5-hydroxy-2-oxapentyl), 1-(5-hydroxy-1-chloromethyl-2-oxapentyl)-, and 1-(6-hydroxy-1-chloromethyl-2-oxahexyl)-2-(trifluoromethylthiomethyl)benzimidazole. They were obtained by condensing the trimethylsilyl derivative of 2-(trifluoromethylthiomethyl) benzimidazole with alkylating agents in the presence of an equimolar mixture of trifluoromethanesulfonic acid and trimethylchlorosilane. These nucleoside analogs showed moderate antiviral activity against some RNA viruses.

Acyclic analogs of nucleosides. Synthesis of hydroxyalkyl derivatives of 2-trifluoromethyl- and 2-trifluoromethylthiobenzimidazole.

1-(2,3-Dihydroxypropyl)-, 1-(4-hydroxy-2-oxabutyl)-, 1-(3-hydroxymethyl-4-hydroxy-2-oxabutyl)-, 1-(1,5-dihydroxy-3-oxa-2-pentyl)-, 1-(5-hydroxy-3-oxa-2-pentyl)-, and 1-(4,5-dihydroxy-2-oxapentyl)-2-trifluoromethyl- and -2-trifluoromethylthiobenzimidazoles were obtained by condensation of trimethylsilyl derivatives of 2-substituted benzimidazoles with alkylating agents in the presence of SnCl4, or by direct alkylation of the sodium salts of the heterocycles.

[Changes in the functional activity and kinetics of macrophages and lymphocytes as affected by retinoic acid]. / Izmenenie funktsional'noi aktivnosti i kinetiki makrofagov i limfotsitov pod deistviem retinoevoi kisloty.

The influence of water-soluble compound of retinoic acid on the ability of human blood cell precursors to transform into macrophages, phagocytic macrophage activity, proliferation and marker properties of lymphocytes and the condition of erythrocytes was studied. It was found that retinoic acid caused erythrocyte damages, induced the ability of precursor cells to transforms into macrophages, did not increase significantly their functional activity and facilitated the formation of granulocyte-lymphocyte-macrophage aggregates. In the absence of macrophages, the action of retinoic acid was followed by the decrease in the expressivity of precursor cells to transform into macrophages, did by the suppression of their proliferative activity.

[The ultrastructural immunomorphology of antibody synthesis]. / K ul'trastrukturnoi immunomorfologii sinteza antitel

Antibody genesis in the lymphoid cells of C3HA mice was studied using horse radish peroxidase as an antigen. The zones of synthesis and concentration of antibodies within the immune competent cells were primarily the perinuclear space and the tubules of the granular endoplasmic reticulum. Less activity of the reaction was exhibited by the lamellar component of the Golgi apparatus and on the polyribosomes.