Subject(s)
Body Temperature Regulation , Epinephrine/physiology , Norepinephrine/physiology , Acclimatization , Blood Pressure/drug effects , Cold Temperature , Energy Metabolism/drug effects , Heart Rate/drug effects , Humans , Isoproterenol/pharmacology , Male , Receptors, Adrenergic, beta/physiologyABSTRACT
The possibility of the routine PIXE measurement of environmental samples has been tested at the INP in Rez. Both thin samples of aerosols collected on membrane filters Synpor and Nuclepore and thick aerosols and fly ashes samples pressed into pellets were analyzed. The Nuclepore filter was found more suitable for PIXE analysis of aerosols than the Synpor, and the thin aerosol sample analysis was found to be easier than the thick sample analysis. The grain size effect in the case of thick fly ash samples is discussed.
Subject(s)
Air Pollutants, Occupational/analysis , Carbon/analysis , Coal/analysis , Industrial Waste/analysis , Coal Ash , Incineration , Indicators and Reagents , Particle Size , Particulate Matter , Reference Standards , Refuse Disposal , Spectrometry, X-Ray Emission , Trace Elements/analysis , UltrafiltrationABSTRACT
Toxicity of mercaptoundecahydro-closo-dodecaborate (MHB, Na2H(11)10B12SH) and accumulation of MHB-derived 10B were studied in E7 neuroblastoma, C6 glioma, HeLa cells and embryonic lung LEP 19 fibroblasts in culture in exponential and stationary phases of growth (2- and 7-day-old cultures, respectively). The pilot study of acute toxicity, performed on C6 glioma cells, showed good tolerance of the drug up to 1000 micrograms/ml (4.8 x 10(-3) M), when cell growth slowed and a small part of the population was lethally damaged (8.3%, 20-h incubation interval). The changes became more extensive and appeared sooner (toward 5 h) at 2000 micrograms MHB/ml (9.5 x 10(-3) M). None of the four cell lines used was found to be affected in gross morphology or growth by 200 micrograms MHB/ml within a 5-day culture interval. When exposed to this dose for 4 h, the amount of 10B accumulated in cell lines at the exponential growth phase ranged from 0.51 to 4.4 ng/micrograms protein; in the stationary cultures of the corresponding cell phenotype, the 10B values were 3 to 10 times lower (0.12-1.2 ng/micrograms protein). Irrespective of the growth phase, the values achieved in C6 glioma cells were several times higher than in the other cell lines. Furthermore, in the glioma cells, particularly in the exponential phase of growth, accumulation of 10B proceeded against the marked concentration gradient. The data provide a new indication for the use of MHB for boron neutron capture therapy of brain tumors.
