Defense Response and Suppression of Phytophthora Blight Disease of Pepper by Water Extract from Spent Mushroom Substrate of Lentinula edodes.

The spent mushroom substrate (SMS) of Lentinula edodes that was derived from sawdust bag cultivation was used as materials for controlling Phytophthora blight disease of pepper. Water extract from SMS (WESMS) of L. edodes inhibited mycelial growth of Phytophthora capsici, suppressed Phytophthora blight disease of pepper seedlings by 65% and promoted growth of the plant over 30%. In high performance liquid chromatography (HPLC) analysis, oxalic acid was detected as the main organic acid compound in WESMS and inhibited the fungal mycelium at a minimum concentration of 200 mg/l. In quantitative real-time PCR, the transcriptional expression of CaBPR1 (PR protein 1), CaBGLU (ß-1,3-glucanase), CaPR-4 (PR protein 4), and CaPR-10 (PR protein 10) were significantly enhanced on WESMS and DL-ß-aminobutyric acid (BABA) treated pepper leaves. In addition, the salicylic acid content was also increased 4 to 6 folds in the WESMS and BABA treated pepper leaves compared to water treated leaf sample. These findings suggest that WESMS of L. edodes suppress Phytophthora blight disease of pepper through multiple effects including antifungal activity, plant growth promotion, and defense gene induction.

Oxalic Acid from Lentinula edodes Culture Filtrate: Antimicrobial Activity on Phytopathogenic Bacteria and Qualitative and Quantitative Analyses.

The culture filtrate of Lentinula edodes shows potent antimicrobial activity against the plant pathogenic bacteria Ralstonia solanacearum. Bioassay-guided fractionation was conducted using Diaion HP-20 column chromatography, and the insoluble active compound was not adsorbed on the resin. Further fractionation by high-performance liquid chromatography (HPLC) suggested that the active compounds were organic acids. Nine organic acids were detected in the culture filtrate of L. edodes; oxalic acid was the major component and exhibited antibacterial activity against nine different phytopathogenic bacteria. Quantitative analysis by HPLC revealed that the content of oxalic acid was higher in the water extract from spent mushroom substrate than in liquid culture. This suggests that the water extract of spent L. edodes substrate is an eco-friendly control agent for plant diseases.

Water Extract from Spent Mushroom Substrate of Hericium erinaceus Suppresses Bacterial Wilt Disease of Tomato.

Culture filtrates of six different edible mushroom species were screened for antimicrobial activity against tomato wilt bacteria Ralstonia solanacearum B3. Hericium erinaceus, Lentinula edodes (Sanjo 701), Grifola frondosa, and Hypsizygus marmoreus showed antibacterial activity against the bacteria. Water, n-butanol, and ethyl acetate extracts of spent mushroom substrate (SMS) of H. erinaceus exhibited high antibacterial activity against different phytopathogenic bacteria: Pectobacterium carotovorum subsp. carotovorum, Agrobacterium tumefaciens, R. solanacearum, Xanthomonas oryzae pv. oryzae, X. campestris pv. campestris, X. axonopodis pv. vesicatoria, X. axonopodis pv. citiri, and X. axonopodis pv. glycine. Quantitative real-time PCR revealed that water extracts of SMS (WESMS) of H. erinaceus induced expressions of plant defense genes encoding ß-1,3-glucanase (GluA) and pathogenesis-related protein-1a (PR-1a), associated with systemic acquired resistance. Furthermore, WESMS also suppressed tomato wilt disease caused by R. solanacearum by 85% in seedlings and promoted growth (height, leaf number, and fresh weight of the root and shoot) of tomato plants. These findings suggest the WESMS of H. erinaceus has the potential to suppress bacterial wilt disease of tomato through multiple effects including antibacterial activity, plant growth promotion, and defense gene induction.

[Clinical picture of early congenital syphilis in 2,5-month-old infant]. / Obraz kliniczny kily wrodzonej wczesnej u 2,5-miesiecznego niemowlecia.

The case of congenital syphilis in a 2.5-month-old infant was describe in the article. Missing information about possibility of infection caused severe course of the disease the and a long-term of treatment.

Immunolocalization of proliferating cells in the rabbit iliac artery after balloon angioplasty.

PURPOSE: Experiments were performed to characterize the location of proliferating cells in the balloon-dilated rabbit iliac artery. MATERIALS AND METHODS: Balloon angioplasty was performed on the external iliac arteries in each of four rabbits. The arteries were removed 3 days later, frozen, cryosectioned, and immunostained with Ki-67, an antibody that identifies proliferating cells. The sections were then examined to determine the patterns of cell proliferation within the arterial media and the ratio of proliferating to nonproliferating cells. RESULTS: Of the 31 arterial cross-sections examined, cell proliferation was circumferential in five (16%), and focal in 26 (84%). Of the 86 foci of proliferation examined within the 31 cross-sections, proliferation was localized to the inner media in 30 (35%), to the outer media in four (5%), and was transmural in 52 (60%). The internal elastica lamina (IEL) appeared normal at 22 foci (26%), but appeared stretched or torn at 64 (74%). Proliferation was usually confined to the inner media at foci having no IEL injury (18 of 22; 82%), but was most often transmural where the IEL was stretched or torn (49 of 64; 77%). The ratio of proliferating to nonproliferating cells, which averaged 0.31 +/- .20, was greater (P < .01) in areas with IEL injury than in areas without IEL injury. CONCLUSION: These results suggest that angioplasty-induced cell proliferation is typically focal rather than circumferential and is associated with stretching or tearing of the IEL.

Relationships among grip strength, work capacity, and recovery.

The relationship between strength, fatigue, and work capacity is a central issue for occupational rehabilitation. Musculoskeletal impairments often are expressed functionally as a loss of strength. For work tasks that require strength, such impairments may have a detrimental effect on work capacity that is sufficient to be disabling. How important is strength as a component of work capacity? How do work capacity and strength affect recovery time from repetitive forceful upper extremity work ? A sample of 40 healthy subjects comprised of 20 males and 20 females was studied in a model that used the isotonic strength of each subject to set exercise levels for subsequent forceful repetitive gripping tasks. Resistance levels of 75%, 50%, and 25% of maximum were used for one-per-second repetitions until the subject could not maintain that pace. Isometric grip strength was measured after 1 minute of rest and after 5, 10, and 20 minutes. Results indicate that males and females do not differ in terms of their rate of recovery and that the best predictor of recovery time is the immediate measurement of strength loss following cessation of work activity.

[Normal values of peak expiratory flow in children from an environment with high degree of atmospheric air pollution]. / Wartosci nalezne (normy) szczytowego przeplywu wydechowego u dzieci ze srodowiska o wysokim stopniu zanieczyszczenia powietrza atmosferycznego.

Peak expiratory flow (PEF) has been measured with Vitalograph (in liters per minute) in 2,512 school-children aged between 7 and 15 years in Upper Silesian Industrial Region. Five hundred eighty one children from Zarki near Czestochowa served as a control group. The results have been analysed statistically. Determined PEF values for children from the Upper Silesian Industrial Region are considered as a biological reference values for assessment of PEF in both health and disease. PEF values calculated for children from Zarki were higher than those in the examined group, except the value for a 14-year old children.

Solving the problem of how medical students solve problems.

Although gathering and processing information are essential to medical problem solving, little is known about what strategies students use to gather information or how they use their cognitive skills to solve problems. We have developed computer-based problem-solving exercises in immunology to determine how students gather and process information. Graphic representations of students' search paths through different problems were developed to visualize how organized and focused their knowledge was, how well their organization related to critical concepts in immunology, where serious misconceptions (confusion or erroneous models) occurred, and whether proper knowledge links between conceptual domains existed. With rapid generation and interpretation of information on patterns and difficulties in problem solving, it should become possible to develop a specific and personal approach to each student's educational needs.

Administering a microcomputer-based problem-solving examination.

Administering a microcomputer-based problem-solving examination in the basic sciences to medical students poses a series of practical concerns not associated with traditional types of testing. This article reviews the issues of planning, student preparation, exam scheduling, student verification, and software security. Also discussed are the effects on student scores of exam scheduling and of students' familiarity with computers, student evaluations of the exam, and security and technical difficulties.

Evaluating preclinical medical students by using computer-based problem-solving examinations.

A study was designed to determine the feasibility of creating and administering computer-based problem-solving examinations for evaluating second-year medical students in immunology and to determine how students would perform on these tests relative to their performances on concurrently administered objective and essay examinations. The results indicate that problem-solving questions of uniform difficulty can be created and that correct solution of the problems correlates with the early selection of the most relevant data. The mean scores on the three types of tests were not significantly different. The scores on the essay and objective examinations were most highly correlated, those on the computer-based and objective examinations were minimally correlated, and those on the computer-based and essay examinations were not correlated.

Audiovisual licensing: purchasing is not owning.

Discusses the use of licensing agreements for health sciences audiovisuals. The difference between productions covered by the copyright law vis-a-vis those with licensing agreements is explored and representative examples of agreements are provided. Health sciences producers have turned to licensing agreements for several reasons including the protection of their productions from illegal duplication. Whereas these agreements may protect producers, they also may unintentionally bar legitimate users from viewing programs and may also create additional work for LRCs and libraries.