ABSTRACT
Sugars such as glucose, maltose, and trehalose, which are metabolized by Dictyostelium discoideum and which enhance vegetative growth, inhibit the development of the slime mold at concentrations which stimulate growth maximally. They block the acquisition of aggregation competence as well as aggregation. The same sugars also inhibit the degradation of preformed glycogen ribonucleic acid, and protein, which is characteristic of development and which occurs when the amoebas are starved by incubation in dilute phosphate buffer.
Subject(s)
Carbohydrates/pharmacology , Dictyostelium/growth & development , Myxomycetes/growth & development , Cell Aggregation/drug effects , Dictyostelium/metabolism , Fungal Proteins/metabolism , Glucose/pharmacology , Glycogen/metabolism , Maltose/pharmacology , Potassium Chloride/pharmacology , RNA/metabolism , Sodium Chloride/pharmacology , Trehalose/pharmacologyABSTRACT
During the differentiation of myxamoebae of Dictyostelium discoideum strain Ax-2 grown in axenic medium there is a seven- to ten-fold increase in the specific activity of cyclic AMP-binding protein(s). Evidence is presented for the belief that cyclic AMP phosphodiesterase and cyclic AMP-binding protein are distinct molecular species.