ABSTRACT
While the poor psychosocial outcomes of young people who have experienced bullying are well known, the harm associated with experiences that do not meet the bullying criteria is not well understood. The aim of this study was to examine the level of harm associated with experiences of peer aggression, as well as bullying, by directly measuring the four elements of intent, perceived harm, repetition and power imbalance that comprise the bullying criteria. The purpose of the study was to establish whether bullying was the most harmful form of peer aggression and whether other types of peer aggression that did not comprise all elements of bullying were comparably harmful. Over 6000 students (aged 11-16) from 10 countries completed a student victimization and aggression questionnaire. Data showed that approximately 50% of participants were not intentionally harmed through peer aggression, although this varied across countries, ranging from 10% in India to 87.5% in Taiwan. In all countries, analyses identified a group that had experienced repeated peer aggression, but with no power imbalance, comparable in size to the bullied group, suggesting that bullying is just "the tip of the iceberg". Victims of bullying self-reported the greatest experiences of harm, although victims of repeated aggression reported comparable harm. The findings show that peer aggression experiences that do not meet the bullying criteria are also rated as harmful by victims. More research is needed to fully understand negative peer interactions that include behaviors outside the scope of the bullying definition, particularly with regard to repeated peer aggression. This study suggests that researchers should consider the level of harm experienced by individuals and avoid terminology such as bullying, while policy makers should place a strong and explicit focus on encompassing a broader realm of harmful peer aggression.
Subject(s)
Aggression/psychology , Self-Injurious Behavior/psychology , Adolescent , Asia , Bullying/psychology , Child , Crime Victims/psychology , Europe , Female , Humans , Intention , Interpersonal Relations , Male , Peer Group , Self Report , Students/psychology , Surveys and QuestionnairesABSTRACT
Axon regeneration after injury in the central nervous system is hampered in part because if an age-dependent decline in the intrinsic axon growth potential, and one of the strategies to stimulate axon growth in injured neurons involves pharmacological manipulation of implicated signaling pathways. Here we report phenotypic cell-based screen of chemical libraries and structure-activity-guided optimization that resulted in the identification of compound 7p which promotes neurite outgrowth of cultured primary neurons derived from the hippocampus, cerebral cortex, and retina. In an animal model of optic nerve injury, compound 7p was shown to induce growth of GAP-43 positive axons, indicating that the in vitro neurite outgrowth activity of compound 7p translates into stimulation of axon regeneration in vivo. Further optimization of compound 7p and elucidation of the mechanisms by which it elicits axon regeneration in vivo will provide a rational basis for future efforts to enhance treatment strategies.
Subject(s)
Acetamides/pharmacology , Axons/drug effects , Drug Discovery , Nerve Regeneration/drug effects , Sulfonamides/pharmacology , Acetamides/chemical synthesis , Acetamides/chemistry , Animals , Cells, Cultured , Dose-Response Relationship, Drug , Mice , Molecular Structure , Rats , Rats, Sprague-Dawley , Structure-Activity Relationship , Sulfonamides/chemical synthesis , Sulfonamides/chemistryABSTRACT
In order to measure the levels of serotonin (5-hydroxyltryptamine, 5-HT), dopamine (DA), 3,4-hydroxyphenylacetic acid (DOPAC), 3-methoxytyramine (3-MT) and homovanillic acid (HVA) simultaneously, an effective derivatization method followed by high-performance liquid chromatography (HPLC) coupled to electrochemical ionization mass spectrometry was used. The derivatization reaction of biological samples with ethyl chloroformate occurred rapidly at room temperature in aqueous conditions, and the resulting derivatives were isocratically separated with good selectivity using a C18 reversed-phase column within 30 min. The study results showed that the new derivatization procedure offers an excellent means of simultaneous determination of 5-HT, DA and their metabolites in mouse brain homogenates, which are important in a number of physiological and behavioral functions.
