Anaerobic digestion of sewage sludge using anaerobic dynamic membrane bioreactor under various sludge composition and organic loading rates.

This study investigates the effects of sludge compositions and organic loading rates (OLRs) on stable biogas production during sludge digestion. Batch digestion experiments evaluate the effects of alkaline-thermal pretreatment and waste activated sludge (WAS) fractions on the biochemical methane potential (BMP) of sludge. A lab-scale anaerobic dynamic membrane bioreactor (AnDMBR) is fed with a mixture of primary sludge and pretreated WAS. Monitoring of volatile fatty acid to total alkalinity (FOS/TAC) helps maintain operational stability. The highest average methane production rate of 0.7 L/L·d is achieved when the OLR, hydraulic retention time, WAS volume fraction, and FOS/TAC ratio are 5.0 g COD/L·d, 12 days, 0.75, and 0.32, respectively. This study finds functional redundancy in two pathways: hydrogenotrophic and acetolactic. An increase in OLR promotes bacterial and archaeal abundance and specific methanogenic activity. These results can be applied to the design and operation of sludge digestion for stable, high-rate biogas recovery.

Sludge disintegration and anaerobic digestion enhancement by alkaline-thermal pretreatment: Economic evaluation and microbial population analysis.

Alkaline-thermal pretreatment was examined for waste activated sludge (WAS) disintegration and subsequent anaerobic digestion (AD). Pretreatment at 60 °C was estimated to provide better economic benefits than higher temperature conditions. The maximum methane yield of 215.6 mL/g COD was achieved when WAS was pretreated at 60 °C and pH 10 for 24 h, which was 46.6% higher than untreated WAS. The pretreatment condition also provided the maximum net savings. The degree of sludge disintegration, considering both loosely bound-extracellular polymeric substance and soluble COD, would be a better indicator to predict anaerobic digestibility than the solubilization rate that considers soluble COD alone. Microbial analysis implied that pretreatment facilitated the growth of hydrolytic bacteria, phyla Bacteroidetes and Firmicutes. In addition, sludge pretreatment enhanced the growth of both acetoclastic and hydrogenotrophic methanogens, genera Methanosaeta and Methanobacterium. The mild AT-PT would be useful to enhance the digestion performance and economic benefit of WAS digestion.