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1.
Epidemiol Infect ; 142(4): 856-60, 2014 Apr.
Article in English | MEDLINE | ID: mdl-23830350

ABSTRACT

SUMMARY We previously revealed that Japanese encephalitis virus (JEV) seroprevalence was 4.5% in pigs on Ishigaki Island from 2005 to 2007. However, a partial E gene sequence (151 bp) of the JEV genome (JEV/sw/Ishigaki/1/2005) was detected in one pig. Phylogenetic analysis showed that JEV/sw/Ishigaki/1/2005 belonged to genotype III and to the same lineages isolated in Taiwan from 2006 to 2008. Serum samples were collected from 128 pigs on Ishigaki from 2009 to 2010, 24 wild boars on Ishigaki from 2008 to 2010, and 117 wild boars on Iriomote Island from 2008 to 2010. Four (3.1%) pigs on Ishigaki were positive for JEV antibody, but all wild boars on the island were negative. Fifty-two (44.4%) wild boars on Iriomote were positive for JEV antibody, in contrast to a seroprevalence of 3.7% in 2000 and 2004. JEV on Iriomote and/or in Taiwan might be related to transmission on Ishigaki.


Subject(s)
Encephalitis, Japanese/epidemiology , Encephalitis, Japanese/veterinary , Swine Diseases/epidemiology , Animals , Antibodies, Viral/blood , Body Weight , Cluster Analysis , Encephalitis Virus, Japanese/isolation & purification , Encephalitis, Japanese/immunology , Encephalitis, Japanese/virology , Islands , Japan/epidemiology , Phylogeny , Seroepidemiologic Studies , Sus scrofa , Swine , Swine Diseases/immunology , Swine Diseases/virology
2.
Endocr J ; 47(3): 239-47, 2000 Jun.
Article in English | MEDLINE | ID: mdl-11036866

ABSTRACT

Activin-A induces increases in FSH secretion, as well as the number of immunoreactive FSH cells, in cultured rat pituitary cells. In this study, we examined whether mechanisms involved in these two actions of activin-A are identical or not, with respect to the involvement of cellular proliferation and of Ca2+-dependent signaling pathways. Treatment with activin-A (25 ng/ml) for 48 h caused increases in the number of cultured rat anterior pituitary cells that incorporated BrdU, a thymidine analog. The stimulatory effects of activin-A on FSH secretion and on the percentage of immunoreactive FSH cells were, however, not inhibited by the presence of the mitotic inhibitor cytosine arabinoside. On the other hand, the stimulatory effect of activin-A on the percentage of immunoreactive FSH cells was completely blocked in the presence of the Ca2+/calmodulin kinase inhibitor KN-62 or the L-type Ca2+ channel blocker nicardipine. Neither of these inhibitors, however, revealed significant influence on the effect of activin-A on FSH secretion. These results suggest that activin-A exhibits its dual effect on FSH cells without causing cellular proliferation. Furthermore, activin-A appears to induce increases in FSH secretion and enlargement of FSH cell population through distinct intracellular signaling pathways, the former through Ca2+-independent and the latter through Ca2+-dependent mechanisms.


Subject(s)
Follicle Stimulating Hormone/metabolism , Growth Substances/pharmacology , Inhibins/pharmacology , Pituitary Gland, Anterior/metabolism , Signal Transduction , 1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine/analogs & derivatives , 1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine/pharmacology , Activins , Animals , Antibody Specificity , Bromodeoxyuridine/metabolism , Calcium Channel Blockers/pharmacology , Calcium Channels/metabolism , Cell Differentiation/drug effects , Cells, Cultured , Dose-Response Relationship, Drug , Follicle Stimulating Hormone/antagonists & inhibitors , Growth Substances/administration & dosage , Humans , Inhibins/administration & dosage , Nicardipine/pharmacology , Pituitary Gland, Anterior/cytology , Pituitary Gland, Anterior/drug effects , Rats
3.
J Vet Med Sci ; 62(7): 731-6, 2000 Jul.
Article in English | MEDLINE | ID: mdl-10945291

ABSTRACT

Pituitary folliculo-stellate (FS) cells were able to modify the effect of activin-A on gonadotropes through the paracrine factor, follistatin. The present study was aimed to examine whether a hypothalamic peptide, pituitary adenylate cyclase activating polypeptide (PACAP), could be a regulator of this paracrine interaction. Co-culture of FS cell-originated cell line TtT/GF cells with rat anterior pituitary cells showed faint inhibitory effect on the stimulatory action of activin-A on FSH secretion. When PACAP was added to the culture during the co-culture period, however, the presence of TtT/GF cells caused significant suppression of the effect of activin-A on FSH secretion. Conditioned-media (CM) from TtT/GF cells, obtained by incubation of TtT/GF cells in the presence or absence of PACAP, were next added to the cultures of anterior pituitary cells alone. CM from TtT/GF cells without PACAP treatment revealed slight, but not significant, suppressive effect on activin-induced increases in FSH secretion and the percentage of FSH cells. Meanwhile, CM from PACAP-treated TtT/GF cells attenuated both effects of activin-A. Furthermore, the inhibitory effect of the CM was neutralized when follistatin antibody was present in the culture. These results suggest that PACAP is able to regulate the paracrine action of FS cells on pituitary gonadotropes. Besides expressing direct actions on pituitary endocrine cells, PACAP may have roles as a regulator of cell-to-cell interactions within the pituitary gland.


Subject(s)
Cell Communication/physiology , Inhibins/pharmacology , Neuropeptides/physiology , Pituitary Gland, Anterior/cytology , Pituitary Gland, Anterior/physiology , Activins , Animals , Cells, Cultured , Coculture Techniques , Culture Media, Conditioned , Estrus , Female , Follicle Stimulating Hormone/metabolism , Growth Substances/pharmacology , Growth Substances/physiology , Neuropeptides/pharmacology , Neurotransmitter Agents/pharmacology , Neurotransmitter Agents/physiology , Pituitary Adenylate Cyclase-Activating Polypeptide , Rats , Rats, Sprague-Dawley
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