Subject(s)
Anticoagulants , Autoanalysis/instrumentation , Calcium/blood , False Positive Reactions , HumansABSTRACT
Serum samples from 954 Irish adults (604 males, 350 females) aged 18 to 65 years were analyzed within 24 hours of receipt for non-fasting total serum cholesterol levels. The subjects were volunteer blood donors (Blood Transfusion Service Board, Pelican House, Dublin) presenting from June 1990 to February 1991. Serum cholesterol increased significantly with age in both males and females. Mean serum cholesterol in males increased from 4.5 (+/- 0.9) mmol/l in those < 25 years, to 5.7 (+/- 1.0) mmol/l at age 55-64 years. In females, mean serum cholesterol increased from 4.3 (+/- 0.9) mmol/l less than 25 years to 6.2 (+/- 0.8) mmol/l at age 55-64 years. Options for reporting cholesterol ranges are discussed. The overall mean cholesterol concentration of 5.1 mmol/l seen in this study is lower than that reported for comparable studies of U.K. and American subjects, but higher than observed in Japanese individuals. Almost 48% of the studied population had a serum cholesterol value less than 5 mmol/l and over 56% had levels less than 5.2 mmol/l.
Subject(s)
Cholesterol/blood , Hypercholesterolemia/prevention & control , Adolescent , Adult , Age Distribution , Aged , Data Collection , Female , Hospitals , Humans , Incidence , Ireland , Male , Mass Screening , Middle Aged , Reference Values , Sex DistributionABSTRACT
Thirty seven cardiac transplants have taken place at the National Cardiac Centre in Ireland since 1985. Data is presented on three still-surviving male patients aged 19 to 42 who received cardiac transplants in 1985 and 1986. Circulating levels of blood cyclosporine were measured by high pressure liquid chromatography and radioimmunoassay; plasma creatinine and bilirubin were also measured. In one of these patients the distribution of cyclosporine in blood was measured by high pressure liquid chromatography in a long term study. For all three patients cyclosporine levels in blood were compared with the daily dose of cyclosporine and biochemical and histopathological parameters.
Subject(s)
Chromatography, High Pressure Liquid/standards , Cyclosporins/pharmacokinetics , Heart Transplantation/immunology , Radioimmunoassay/standards , Adult , Biopsy , Cyclosporins/administration & dosage , Cyclosporins/blood , Evaluation Studies as Topic , Heart Transplantation/pathology , Humans , Male , Myocardium/pathology , Plasma/chemistry , Sensitivity and SpecificityABSTRACT
The individual creatinine kinase (CK) isoenzymes CK-BB and CK-Macro II have previously been investigated as potential tumour markers. We believe there is a need for a system to measure those CK forms not usually present in serum. We have studied a CK-MB immunoinhibition kit which measures all residual CK activity following inactivation of M-subunit activity. In 162 patients with cancer we found no difference in grading (+ or -) between detailed isoenzyme studies and the simple non-M assay. In 33 samples with elevated non-M CK, detailed analysis showed BB alone (45%), Macro II alone (9%), or both (36%). Raised activities were mainly found in patients with small cell lung cancer (SCLC) (17/40; 43%) and GI Tumours (6/11; 55%). In patients with SCLC, elevated activities were associated with disseminated disease. Preliminary evidence indicates that Non-M CK may also be a simple means of monitoring initial treatment response.
Subject(s)
Biomarkers, Tumor/blood , Creatine Kinase/blood , Neoplasms/diagnosis , Humans , IsoenzymesABSTRACT
Recent evidence has suggested that the levels of Apolipoprotein A-1 and B in plasma are better indicators of the risk of atherosclerotic artery disease than plasma lipids. This study describes the measurement of plasma Apolipoprotein A-1 and B by immunoturbidimetry in patients with atherosclerotic artery disease and in normal controls. Apoprotein B and the ratio of Apoprotein A-1/Apoprotein B were found to be the best discriminators between the two groups.
Subject(s)
Apolipoproteins A/analysis , Apolipoproteins B/analysis , Coronary Disease/blood , Adult , Aged , Coronary Disease/epidemiology , Female , Humans , Immunoassay/standards , Immunodiffusion/standards , Lipoproteins/blood , Male , Middle Aged , Nephelometry and Turbidimetry/standards , Reagent Kits, Diagnostic/standards , Reproducibility of Results , Risk FactorsSubject(s)
Carcinoma, Small Cell/enzymology , Creatine Kinase/blood , Lung Neoplasms/enzymology , Cell Line , Humans , IsoenzymesABSTRACT
Cyclosporin A (CyA) was assayed by high performance liquid chromatography (HPLC) following extraction of the whole blood or plasma with diethyl ether and chromatography on a Sep-Pak Silica cartridge. Using carefully chosen volumes and ratios of ethyl acetate and hexane in the eluting sequences on the Sep-Pak, the major interferent present in normal blood and serum was eliminated.
Subject(s)
Cyclosporins/blood , Acetates , Chromatography, High Pressure Liquid/methods , Ether , Hexanes , Humans , SolventsABSTRACT
The usefulness of creatine kinase isoenzymes, measured by a commercially available "kit" method, in the diagnosis of myocardial infarction, was assessed. Raised levels of the heart isoenzyme (MB) were found after exercise and in acute coronary insufficiency but the levels were lower than those found after myocardial infarction. Complete agreement between diagnosis based on creatine kinase isoenzyme measurements alone and diagnosis according to World Health Organization criteria was obtained.
