ABSTRACT
To reveal Herpes simplex virus 2 specific IgG and to determine their avidity the ELISA test-kit was constructed using recombinant protein gG2 (PSC SPC Diaproph-Med). Using this test-kit the distribution of specific antibodies with different avidity indexes was investigated in practically healthy donor samples. It is possible to use the mentioned ELISA test-kit for confirmation of primary infection, and also for differentiation of primary infection, chronic disease and virus reactivation. Thus, this ELISA test-kit could be an additional tool in herpetic infection diagnosis.
Subject(s)
Antibodies, Viral , Antibody Affinity/immunology , Herpes Genitalis/diagnosis , Herpesvirus 2, Human/physiology , Immunoglobulin G , Antibodies, Viral/immunology , Chronic Disease , Cloning, Molecular , Enzyme-Linked Immunosorbent Assay , Escherichia coli , Herpes Genitalis/immunology , Herpes Genitalis/virology , Humans , Immunoglobulin G/immunology , Plasmids , Reagent Kits, Diagnostic , Recombinant Proteins/genetics , Recombinant Proteins/immunology , Severity of Illness Index , Virus ActivationABSTRACT
The effect of continuous low-intensity and acute high-intensity ionizing radiation in low and high doses on expression of cytochrome P450 2El (1.14.14.1) in mice liver was studied. It was shown, that changes of Cyp2e1 amount depended on the dose and intensity of ionizing radiation. Low doses of continuous gamma-radiation caused reliable decrease of Cyp2e1 expression on protein mRNA levels. Low doses of acute gamma-radiation lead to an increase of Cyp2el amount, while high doses--to a corresponding decrease. We showed, that low and high doses of acute gamma-radiation caused a significant decrease of Cyp2e1 mRNA level. We suppose that the detected changes of Cyp2el expression are associated with peroxidation process and development of oxidative stress.
Subject(s)
Cytochrome P-450 CYP2E1/biosynthesis , Gamma Rays/adverse effects , Liver/radiation effects , Animals , Blotting, Northern , Blotting, Western , Cytochrome P-450 CYP2E1/genetics , Dose-Response Relationship, Radiation , Electrophoresis, Agar Gel , Gene Expression/radiation effects , Lipid Peroxidation , Liver/enzymology , Male , Mice , Mice, Inbred BALB C , Oxidative Stress , RNA, Messenger/biosynthesis , RNA, Messenger/genetics , Radiation DosageABSTRACT
In the experiments in vitro using the primary mononuclear cells (MNC) culture of the human peripheral blood the influence of interferonogenic yeast RNA-tilorone molecular complex on the DNA, RNA and protein synthesis was studied. The complex was shown to inhibit the insertion of 3H-thymidine, 3H-uridine and 3H-leucine into DNA, RNA and protein of MNC total pool (by 13, 1 and 40% respectively); that was practically conformed with this synthesis inhibition upon to a natural origin polynucleotide interferon inducers--lariphan (9, 0 and 57% respectively) and ridostin (9, 0 and 56% respectively) action, and at the same time rather less than poly(I)-poly(C) (14, 5 and 62% respectively). In the case of preliminary cell stimulation by the mitogen PHA the complex revealed comitogenic action at a concentration 25 micrograms/ml, that corresponded to optimal for interferonogenesis; the increase of the doses till 100-1000 micrograms/ml lead to in the reversal effect. To proceed from mutual relation between interferonogen preparations influence on the mentioned synthesis and their cytotoxicity the conclusion was about made the complex promising usage as an interferon inducer both in vitro and in vivo conditions.
