¿Son las células mioepiteliales el principal componente en los adenomas pleomórficos? / Myoepithelial cells are the main component in pleomorphic adenomas?

Objetivo: El objetivo de este estudio fue cuantificar por medio de inmunohistoquí-mica el número de células mioepiteliales (CMs) en adenomas pleomorfos (APs). Mate-rial y Métodos: Se recuperaron los cubos de 27 APs y se hicieron nuevas laminillas, las que se tiñeron con un anticuerpo anti-proteína S-100, se contó el número de células S-100 positivas, se registró su morfología y se hicieron comparaciones del número de CMs tomando en cuenta el sexo, edad y glándula de origen. Resultados: Se observó que en el tejido glandular normal, las CMs solo se observaron alrededor de las estruc-turas ductuales. En los APs analizados se encontró que en promedio, solamente el 27,4% de las células neoplásicas fueron positivas a este anticuerpo. Con excepción de un AP, en todos los casos analizados las células plasmocitoides fueron las células más comúnmente encontradas (48,6%). Conclusiones: Los resultados de este estudio su-gieren que las CMs no forman el componente celular principal del compartimiento neo-plásico de los APs y confirman las evidencias encontradasdesde hace varios años, por diferentes autores, quienes estudiando los APs, sugirieron que las CMs no forman la mayor parte de las células neoplásicas en estas entidades

Objective: The aim of this study was to quantify by immunohistochemistry the number of myoepithelial cells (MyECs) in pleomorphic adenomas (PAs). Material and methods: We retrieved the paraffin cubes of 27 PAs, new slides were done and they were stained with anti-S100 protein antibody. The amount of S-100 protein positive cells was quantified, their morphology was recorded and comparison among MyEC number with age, gender and involved gland were also done. Results: With S-100 protein, MyECs in normal salivary gland tissue were seen surrounding the ductual structures only. In the analysed PAs a mean of 27.4% of the neoplastic cells were positive to the antibody. With the exception of one PA, in all the analysed cases the plasmacytoid cells were the most commonly identified cells (48,6%). Conclusions: Results of this study suggest that MyECs do not constitute the main cellular component of the neoplastic compartment in PAs and corroborate the previously reported evidence by different au-thors, who studying the PAs suggested that MyECs does not comprise the main cellular neoplastic component of these entities

Myoepithelial cells are the main component in pleomorphic adenomas?

OBJECTIVE: The aim of this study was to quantify by immunohistochemistry the number of myoepithelial cells (MyECs) in pleomorphic adenomas (PAs). MATERIAL AND METHODS: We retrieved the paraffin cubes of 27 PAs, new slides were done and they were stained with anti-S100 protein antibody. The amount of S-100 protein positive cells was quantified, their morphology was recorded and comparison among MyEC number with age, gender and involved gland were also done. RESULTS: With S-100 protein, MyECs in normal salivary gland tissue were seen surrounding the ductual structures only. In the analysed PAs a mean of 27.4% of the neoplastic cells were positive to the antibody. With the exception of one PA, in all the analysed cases the plasmacytoid cells were the most commonly identified cells (48,6%). CONCLUSIONS: Results of this study suggest that MyECs do not constitute the main cellular component of the neoplastic compartment in PAs and corroborate the previously reported evidence by different authors, who studying the PAs suggested that MyECs does not comprise the main cellular neoplastic component of these entities.