ABSTRACT
Summary: Background. Climate conditions in the northwest of Spain are from the rest of the country, and the pollen sensitisation rates and allergens involved are different. The present study aimed to investigate the sensitisation profile of patients with grass pollen allergy and the interference of other sensitisations in respiratory symptoms. Methods. A total of 959 Spanish patients with seasonal respiratory symptoms and a positive skin prick test (SPT) to Phleum pratense pollen were studied. Patients were classified as having rhinitis and/or bronchial asthma. A battery of SPTs, including common weeds and tree pollens, profilin, polcalcin, moulds, Dermatophagoides pteronyssinus, Lepidoglyphus destructor, and cat and dog dander were performed. Serum specific IgE (sIgE) to Phl p 1 and Phl p 5, adding sIgE to Phl p 7, Phl p 12 and house dust mites (HDMs) or other pollens in selected cases were measured. Results.The majority (89.8%) of the patients were polysensitised according to SPT. HDM co-sensitisation was the most prevalent (62.3%). Profilin and polcalcin rendered a positive result in 25.9% and 18.7% of the patients, respectively. A higher proportion of patients recognized sIgE to Phl p 1 (88.7%) with respect to Phl p 5 (59%). Phl p 1-sIgE levels were higher than Phl p 5-sIgE levels, and no differences were found in patients with rhinitis and/or asthma. However, total serum IgE was higher in patients with asthma. Multivariate regression analyses revealed that only sIgE to Dermatophagoides pteronyssinus (after adjusting by sIgE to Phl p 1, Phl p 5 and Lepidoglyphus destructor) was associated with a greater risk of asthma. Conclusions. Phl p 1 is the most relevant allergen in patients with grass pollen allergy in the northwest of Spain. Sensitisation rates against panallergens are low. Even in patients with grass pollen allergy, HDM sensitisation plays a relevant role in asthma.
Subject(s)
Allergens/immunology , Antigens, Dermatophagoides/immunology , Arthropod Proteins/immunology , Asthma , Dermatophagoides pteronyssinus/immunology , Phleum , Pollen/immunology , Rhinitis , Animals , Asthma/immunology , Dogs , Humans , Hypersensitivity , Immunoglobulin E/blood , Immunoglobulin E/immunology , Plant Proteins , Poaceae , Profilins , Rhinitis/immunology , Rhinitis, Allergic, Seasonal/diagnosis , Rhinitis, Allergic, Seasonal/epidemiology , Skin Tests/methodsABSTRACT
OBJECTIVE: To define the sensitization pattern of patients with anaphylaxis to Vespa velutina nigrithorax (VVN). METHODS: We studied 100 consecutive Spanish patients with anaphylaxis to Hymenoptera venom and systematically determined specific IgE (sIgE) to whole venoms (Vespula species, Polistes dominula, Apis mellifera, Vespa crabro, and Dolichovespula maculata) and their molecular components (rApi m 1, rApi m 5, rApi m 10, rVes v 1, rVes v 5, rPol d 5, and cross-reactive carbohydrates). Specific IgE to VVN venom and its antigen 5 (nVesp v 5) were measured in a subsample. RESULTS: Seventy-seven patients had anaphylaxis to VVN. Of these, only 16 (20.8%) reported previous VVN stings, but were stung by other Hymenoptera. Positive sIgE (>0.35 kUA/L) to each of the whole venoms was detected in >70% of patients (Vespula species in 100%). The components showing >50% positivity were rApi m 5 (51.4%), rPol d 5 (80.0%), and rVes v 5 (98.7%). This pattern was similar to that of Vespula species anaphylaxis (n=11) but different from that of A mellifera anaphylaxis (n=10). Specific IgE to nVesp v 5 was positive in all patients (n=15) with VVN anaphylaxis and was correlated with sIgE to both rVes v 5 (R=0.931) and rPol d 5 (R=0.887). CONCLUSIONS: VVN has become the commonest cause of Hymenoptera anaphylaxis in our area. Most cases report no previous VVN stings. Their sensitization pattern is similar to that of patients with anaphylaxis to other Vespidae. Specific IgE to antigen-5 from VVN, Vespula species, and P dominula are strongly correlated in patients with VVN anaphylaxis.
Subject(s)
Allergens/immunology , Anaphylaxis/immunology , Hypersensitivity/immunology , Insect Bites and Stings/immunology , Wasp Venoms/immunology , Adult , Aged , Aged, 80 and over , Anaphylaxis/epidemiology , Animals , Female , Humans , Hypersensitivity/epidemiology , Immunization , Immunoglobulin E/metabolism , Insect Bites and Stings/epidemiology , Male , Middle Aged , Spain/epidemiology , Wasps , Young AdultABSTRACT
Objective: To define the sensitization pattern of patients with anaphylaxis to Vespa velutina nigrithorax (VVN). Methods: We studied 100 consecutive Spanish patients with anaphylaxis to Hymenoptera venom and systematically determined specific IgE (sIgE) to whole venoms (Vespula species, Polistes dominula, Apis mellifera, Vespa crabro, and Dolichovespula maculata) and their molecular components (rApi m 1, rApi m 5, rApi m 10, rVes v 1, rVes v 5, rPol d 5, and cross-reactive carbohydrates). Specific IgE to VVN venom and its antigen 5 (nVesp v 5) were measured in a subsample. Results: Seventy-seven patients had anaphylaxis to VVN. Of these, only 16 (20.8%) reported previous VVN stings, but were stung by other Hymenoptera. Positive sIgE (>0.35 kUA/L) to each of the whole venoms was detected in >70% of patients (Vespula species in 100%). The components showing >50% positivity were rApi m 5 (51.4%), rPol d 5 (80.0%), and rVes v 5 (98.7%). This pattern was similar to that of Vespula species anaphylaxis (n=11) but different from that of A mellifera anaphylaxis (n=10). Specific IgE to nVesp v 5 was positive in all patients (n=15) with VVN anaphylaxis and was correlated with sIgE to both rVes v 5 (R=0.931) and rPol d 5 (R=0.887). Conclusions: VVN has become the commonest cause of Hymenoptera anaphylaxis in our area. Most cases report no previous VVN stings. Their sensitization pattern is similar to that of patients with anaphylaxis to other Vespidae. Specific IgE to antigen-5 from VVN, Vespula species, and P dominula are strongly correlated in patients with VVN anaphylaxis (AU)
Subject(s)
Humans , Animals , Male , Female , Young Adult , Adult , Middle Aged , Aged , Aged, 80 and over , Allergens/immunology , Anaphylaxis/immunology , Hypersensitivity/immunology , Insect Bites and Stings/immunology , Wasps , Wasp Venoms/immunology , Cross-Sectional Studies , Anaphylaxis/epidemiology , Hypersensitivity/epidemiology , Immunoglobulin E/immunology , Insect Bites and Stings/epidemiology , Spain/epidemiologySubject(s)
Allergens/immunology , Amniotic Fluid/immunology , Galactose/immunology , Hypersensitivity/immunology , Occupational Diseases/immunology , Occupational Exposure/adverse effects , Adult , Animals , Cattle , Female , Galactose/adverse effects , Humans , Hypersensitivity/diagnosis , Immunoglobulin E/blood , Immunoglobulin E/immunology , Immunoglobulin M/immunology , Male , Middle Aged , Occupational Diseases/diagnosisABSTRACT
No disponible
Subject(s)
Humans , Male , Female , Adult , Middle Aged , Amniotic Fluid/immunology , Galactose/analysis , Urticaria/diagnosis , Urticaria/immunology , Electrophoresis, Polyacrylamide Gel/methods , Hypersensitivity, Immediate/complications , Hypersensitivity, Immediate/immunology , Immunoglobulin E/analysisSubject(s)
Anaphylaxis/immunology , Disaccharides/administration & dosage , Disaccharides/adverse effects , Drug Hypersensitivity/immunology , Administration, Intravaginal , Aged , Anaphylaxis/diagnosis , Anaphylaxis/drug therapy , Anti-Allergic Agents/therapeutic use , Antifungal Agents/administration & dosage , Capsules , Disaccharides/immunology , Drug Compounding , Drug Hypersensitivity/diagnosis , Drug Hypersensitivity/drug therapy , Epitopes , Female , Humans , Imidazoles/administration & dosage , Intradermal Tests , Treatment OutcomeSubject(s)
Anaphylaxis/immunology , Coloring Agents/adverse effects , Drug Hypersensitivity/immunology , Methylene Blue/adverse effects , Rosaniline Dyes/adverse effects , Aged , Aged, 80 and over , Anaphylaxis/diagnosis , Drug Hypersensitivity/diagnosis , Female , Humans , Intradermal Tests , Male , Middle Aged , Plasma , Predictive Value of Tests , Risk Factors , Rosaniline Dyes/immunology , Sentinel Lymph Node Biopsy/adverse effectsABSTRACT
No disponible
Subject(s)
Humans , Male , Female , Middle Aged , Aged, 80 and over , Methylene Blue/adverse effects , Azure Stains/adverse effects , Immunoglobulin A/immunology , IgA Deficiency/immunology , Anaphylaxis/complications , Anaphylaxis/immunology , Dermatitis, Contact/complications , Dermatitis, Contact/immunology , Skin Tests/methods , Urticaria/complications , Urticaria/immunologyABSTRACT
No disponible
Subject(s)
Humans , Female , Middle Aged , Anaphylaxis/diagnosis , Anaphylaxis/immunology , Cetuximab/adverse effects , Hypersensitivity, Immediate/complications , Hypersensitivity, Immediate/diagnosis , Vaginal Creams, Foams, and Jellies/adverse effects , Antifungal Agents/adverse effects , Administration, Intravaginal , Azoles/adverse effects , Skin Tests/methods , Skin Tests , Vulvovaginitis/complications , Vulvovaginitis/drug therapySubject(s)
Antifungal Agents/adverse effects , Drug Eruptions/etiology , Fluconazole/adverse effects , Herpesviridae Infections/virology , Vaginitis/drug therapy , Adult , Antifungal Agents/immunology , Diagnosis, Differential , Drug Eruptions/diagnosis , Drug Eruptions/immunology , Female , Fluconazole/immunology , Herpesviridae Infections/diagnosis , Herpesviridae Infections/immunology , Humans , Intradermal Tests , Predictive Value of Tests , Risk Factors , Vaginitis/diagnosis , Vaginitis/microbiologyABSTRACT
No disponible