ABSTRACT
The preparation of a new beta-tetrasubstituted porphyrin through a short synthesis with liquid crystalline properties in a wide range of temperatures including room temperature is described.
ABSTRACT
A group of new 4-[2-(7-heterocyclemethoxynaftalen-2-ylmethoxy)ethyl]benzoic acids have been synthesized and pharmacologically evaluated as LTD(4)-antagonists. Thiazole derivatives, especially 4-[2-[7-(4-cyclobutylthiazole-2-ylmethoxyl)naphthalen- 2-ylmetho-xy]et hyl]benzoic acid, present considerable activity and improved pharmacokinetic profiles in comparison with our quinoline containing lead molecule confirming the interest of our compounds as potentially oral antiasthmatics and that the 4-alkylthiazole system can be considered as bioisosteric of the quinoline ring at least in our series of compounds.
Subject(s)
Benzoates/chemical synthesis , Benzoates/pharmacology , Leukotriene D4/antagonists & inhibitors , Animals , Benzoates/chemistry , Benzoates/pharmacokinetics , Drug Evaluation , Guinea Pigs , Male , MiceABSTRACT
After experimental conditions were established, 366 strains of mycobacteria belonging to 23 different species were studied for fatty acids, secondary alcohols, and mycolic acid cleavage products by capillary gas-liquid chromatography. Additionally, the mycolic acid pattern was studied by thin-layer chromatography. Capillary gas-liquid chromatography allowed direct identification of the following Mycobacterium spp.: M. kansasii, M. marinum, M. szulgai, M. xenopi, M. malmoense, and M. gordonae. The patterns of mycolic acid methyl esters recorded for the test strains of M. chelonae and M. agri may be of value in the identification of these species. Moreover, the combined use of the two chromatographic techniques provided precise identification of the M. tuberculosis complex, M. simiae, M. fallax, M. triviale, and M. chelonae-like organisms. A minimal set of biochemical tests is usually required to obtain identification to the species level when chromatographic procedures alone are not sufficient. Under the reported experimental conditions, thin-layer chromatography and capillary gas-liquid chromatography are rapid and very useful techniques for the identification of mycobacteria.
Subject(s)
Chromatography, Gas/methods , Mycobacterium/classification , Alcohols/analysis , Chromatography, Thin Layer/methods , Evaluation Studies as Topic , Fatty Acids/analysis , Mycobacterium/growth & development , Mycolic Acids/analysis , Reproducibility of Results , Species SpecificityABSTRACT
A fast-growing, non-photochromogenic mycobacterium isolated from the environment exhibited, on thin-layer chromatograms, a characteristic pattern of mycolates composed of unsaturated mycolates and also an unknown more polar component. Spectroscopic analysis and chemical degradation showed that this latter component was a novel mycolic acid containing a methoxy group at the omega-1 position (instead of omega-17 and omega-18 in known methoxymycolates), and two double bonds in the long mero aldehyde chain (instead of one as in known mycolates with additional oxygenated groups).
Subject(s)
Mycobacterium/analysis , Mycolic Acids/analysis , Water Microbiology , Magnetic Resonance Spectroscopy , Mass Spectrometry , Mycolic Acids/chemistryABSTRACT
C8H12N+.C4H5O6-, Mr = 271.27, monoclinic, P21, a = 6.352 (2), b = 14.195 (5), c = 7.507 (2) A, beta = 107.08 (2) degrees, V = 647.0 (8) A3, Z = 2, Dx = 1.39 g cm-3, lambda (Mo K alpha) = 0.71069 A, mu (Mo K alpha) = 1.05 cm-1, F(000) = 288, T = 294 K, R = 0.037 for 941 observed reflections [F greater than 2 sigma (F)]. This (+)-tartrate structure is very similar to its meso-tartrate analogue. O(6) occupies an unusual antiperiplanar position relative to the carboxyl group. A strong hydrogen-bond network stabilizes the crystal packing.
