Effect of methods application of copper nanoparticles in the growth of avocado plants.

The aim of this greenhouse study was to evaluate root irrigation, foliar spray, and stem injection in order to find the best method for the nanofertilization of avocado plants with green synthesized CuNPs. One-year-old avocado plants were supplied four times (every 15 days) with 0.25 and 0.50 mg/ml of CuNPs through the three fertilization methods. Stem growth and new leaf formation were evaluated over time and after 60 days of CuNPs exposure, several plant traits (root growth, fresh and dry biomass, plant water content, cytotoxicity, photosynthetic pigments, and total Cu accumulation in plant tissues) were evaluated for CuNPs improvement. Regarding the control treatment, stem growth and new leaf appearance were increased by 25 % and 85 %, respectively, by the CuNPs supply methods of foliar spray>stem injection>root irrigation, with little significant differences among NPs concentrations. Avocado plants supplied with 0.25 and 0.50 mg/ml CuNPs maintained a hydric balance and cell viability ranged from 91 to 96 % through the three NPs application methods. TEM did not reveal any ultrastructural organelle changes induced by CuNPs in leaf tissues. The concentrations of CuNPs tested were not high enough to exert deleterious effects on the photosynthetic machinery of avocado plants, but photosynthetic efficiency was also found to be improved. The foliar spray method showed improved uptake and translocation of CuNPs, with almost no loss of Cu. In general, the improvement in plant traits indicated that the foliar spray method was the best for nanofertilization of avocado plants with CuNPs.

Evaluation of in situ biosurfactant production by inoculum of P. putida and nutrient addition for the removal of polycyclic aromatic hydrocarbons from aged oil-polluted soil.

This work aimed to conduct a laboratory study to evaluate the use of Pseudomonas putida CB-100 and nutrient addition for the removal of PAHs from an aged oil-polluted soil of Veracruz, Mexico. Pseudomonas putida is a biosurfactant-producing bacterium capable of metabolizing polycyclic aromatic hydrocarbons (PAHs), which are toxic compounds with low water solubility, high melting, and boiling points, and low vapor pressure; characteristics that increase as their molecular weight increases and make them more recalcitrant. The methodology consisted in sampling the long-term oil-polluted soil and testing the use of Gamma irradiation (25 kGy) for the sterilization of the soil for abiotic control. We evaluated serological bottles containing 20 g of 35% moist soil (irradiated and non-irradiated) with the following treatments: the addition of nutrients (NH4Cl, NaNO3, KH2PO4, and K2HPO4), an inoculum of P. putida, and both P. putida and nutrients. The parameters assessed were pH, organic matter, humidity, available phosphorus, total nitrogen, cultivable heterotrophic microorganisms, CO2 production, rhamnolipids, surface tension, and the removal of eleven PAHs. The non-irradiated soil added with P. putida was the most efficient in the removal of PAHs; the pattern was: Benzo(a)anthracene > Phenanthrene > Fluoranthene > Benzo(k)fluoranthene > Chrysene > Pyrene > Anthracene > Acenaphthylene > Benzo(b)fluoranthene. In conclusion, P. putida in the non-irradiated soil produced in situ biosurfactants (1.55 mg/kg of rhamnolipids and an 11.9 mN/m decrease in surface tension) and removed PAHs in 10 days.

Phytotoxicity and upper localization of Ag@CoFe2O4 nanoparticles in wheat plants.

Environmental concern related to Ag+ release from conventional AgNPs is expected to be prevented once contained into a magnetic core like magnetite or CoFe2O4. Accordingly, we obtained CoFe2O4 NPs by microwave-assisted synthesis, which AgNO3 addition rendered Ag@CoFe2O4 NPs. NPs were characterized, and before exploring potential applications, we carried out 7-day wheat toxicity assays. Seed germination and seedling growth were used as toxicity endpoints and photosynthetic pigments and antioxidant enzymes as oxidative stress biomarkers. Total Fe, Co, and Ag determination was initial indicative of Ag@CoFe2O4 NPs uptake by plants. Then NPs localization in seedling tissues was sought by scanning electron microscopy (SEM) and darkfield hyperspectral imaging (DF-HSI). Not any silver ion (Ag+) was detected into the ferrite structure, but results only confirmed the presence of metallic silver (Ag0) adsorbed on the CoFe2O4 NPs surface. Agglomerates of Ag@CoFe2O4 NPs (~10 nm) were fivefold smaller than CoFe2O4 NPs, and ferrimagnetic properties of the CoFe2O4 NPs were conserved after the formation of the Ag@CoFe2O4 composite NPs. Seed germination was not affected by NPs, but root and shoot lengths of seedlings diminished 50% at 54.89 mg/kg and 168.18 mg/kg NPs, respectively. Nonetheless, hormesis was observed in roots of plants exposed to lower Ag@CoFe2O4 NPs treatments. Photosynthetic pigments and the antioxidant enzymes catalase (CAT), superoxide dismutase (SOD), guaiacol peroxidase (GPX), and ascorbate peroxidase (APX) indicated oxidative damage by reactive oxygen species (ROS) generation. SEM suggested NPs presence in shoots and roots, whereas DF-HSI confirmed some Ag@CoFe2O4 NPs contained in shoots of wheat plants.