ABSTRACT
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Subject(s)
Humans , Ischemia/surgery , Neovascularization, Physiologic , Vascular Surgical Procedures/methods , Ischemia/physiopathology , Vascular Patency , Vascular CapacitanceABSTRACT
La isquemia de larga duración es un proceso bastante común durante y tras la cirugía vascular, y la correcta recuperación de la función del órgano afectado depende de que se recupere el flujo sanguíneo del órgano afectado. La isquemia desencadena procesos como la inflamación, angiogénesis o vasculogénesis, que permitirán la reperfusión del órgano. Endoglina es un correceptor para los miembros de la superfamilia de citocinas de TGF-β. En nuestro laboratorio llevamos muchos años estudiando la función y los mecanismos de acción de endoglina, así como su implicación en regulación del tono vascular, angiogénesis, inflamación, fibrosis y en enfermedades como la preeclampsia y la hipertensión. Nuestro objetivo en este trabajo es revisar cómo se suceden los eventos que tienen lugar en la revascularización postisquémica, y mostrar las evidencias que colocan a endoglina como una molécula clave en estos eventos y como una posible diana terapéutica
Long-term ischemia is frequent during and after vascular surgery, and the recovery of affected organ function depends on the re-establishment of blood supply. Ischemia triggers processes such as inflammation, angiogenesis, or vasculogenesis that will lead to tissue remodeling and blood supply restoration. All these events are closely related and highly regulated to achieve complete recovery. Endoglin is a co-receptor for TGF-β cytokines superfamily that plays a central role in vascular physiology, angiogenesis, inflammation and post-ischemic blood supply recovery. Our laboratory has been involved for a long time in the study of endoglin function and its mechanisms of action and involvement in the regulation of vascular function, angiogenesis, inflammation, and fibrosis, as well as in diseases such as hypertension and preeclampsia. The aim here is to review how post-ischemic revascularization takes place, and to assess the role of endoglin in these events, and its importance as a possible therapeutic target
Subject(s)
Humans , Reperfusion/methods , Ischemia/surgery , Neovascularization, Physiologic/physiology , Lymphotoxin-alpha/physiology , Biomarkers/analysis , Inflammation/physiopathology , Cytokines/physiologyABSTRACT
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Subject(s)
Humans , Fibrosis/etiology , Neoplasms, Muscle Tissue/pathology , Epithelial Cells/pathology , Kidney/pathology , Cell Polarity , Extracellular MatrixABSTRACT
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Subject(s)
Humans , DNA/therapeutic use , RNA, Messenger/therapeutic use , Transcription, Genetic , RNA Interference , Gene Silencing , RNA, Double-Stranded , RNA-Induced Silencing ComplexABSTRACT
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Subject(s)
Male , Female , Aged , Humans , Hypertension/epidemiology , Mass Screening , Hypertension/diagnosis , Hypertension/drug therapy , Age Factors , Aging , Blood Pressure Determination/methods , Antihypertensive Agents/therapeutic use , Glomerulosclerosis, Focal Segmental/physiopathologyABSTRACT
Objetivo: analizar la relación entre las variables del estrés oxidativo y las variables psicológicas y sociales. Material y método: siguiendo una metodología correlacional se han analizado las relaciones entre las variables de los procesos oxidativos y las emocionales, cognitivas y sociales. Los datos se han recogido en el contexto de una intervención nutricional controlada según aparece descrita en un trabajo previo. Resultados: se estimaron las correlaciones a partir de las 63 mediciones efectuadas en 21 sujetos. Se han encontrado correlaciones significativas (p > 0,01) entre la glutatión reductasa y la depresión, la no satisfacción con la soledad y la actitud hacia el propio envejecimiento. También han aparecido correlaciones significativas (p > 0,05) entre las sustancias que reaccionan con el ácido tiobarbitúrico, y la red y el apoyo social. Sin embargo, el patrón más consistente de relación se ha encontrado entre la satisfacción con las actividades de la residencia (R2 = 0,30, p < 0,001) y los productos finales de la oxidación, las concentraciones de ácido tiobarbitúrico en el plasma y en la membrana de los eritrocitos, y las variables antioxidantes como la glutatión reductasa y las sustancias antioxidantes totales. No se encontraron correlaciones significativas entre las variables relacionadas con los productos finales de la oxidación y las variables cognitivas. Conclusión: la conclusión más relevante del estudio es la relación entre el estrés oxidativo y las variables emocionales de satisfacción con el contexto próximo
Objective: to analyse the associations among oxidative stress variables and psychological and social variables. Material and method: the associations among oxidative stress variables and emotional, cognitive and social variables were studied using a correlational method. Data were gathered in the context of a controlled nutritional intervention, as described in a previous study. Results: correlations were estimated based on 63 measurements carried out in 21 subjects. Significant correlations (p > 0.01) were found among glutation reductase (GR) and depression, dissatisfaction with solitude and attitude towards ageing. Significant correlations (p > 0.05) were also found between substances that react with thiobarbituric acid (TBARS) and social networks and support. However, the most consistent pattern of associations was found between satisfaction with the activities of the nursing home (R2 = 0.30 p < 0.001) and oxidation end-products: TBARSP in plasma and TBARSM in erythrocyte membrane and antioxidant variables such as GR and total antioxidant substances. No significant correlations were found among variables related to oxidation end-products and cognitive variables. Conclusion: The most important conclusion of this study is the relationship found between oxidative stress and the variables of emotional satisfaction with the immediate environment
Subject(s)
Male , Female , Aged , Humans , Oxidative Stress/physiology , Aging/physiology , Psychosocial Deprivation , Cognition Disorders/physiopathology , Glutathione Reductase/analysis , Thiobarbiturates/analysis , Antioxidants/pharmacokinetics , Aging/psychology , Personal Satisfaction , Depression/physiopathology , Social Support , Homes for the Aged/statistics & numerical dataABSTRACT
Chronic renal disease is characterized by the accumulation of extracellular matrixproteins in the kidney and a loss of renal function. Tubulointerstitial fibrosis hasbeen reported to play an important role in the progression of chronic renal diseases.Transforming growth factor-beta1 (TGF-alpha1) is a profibrotic cytokine playing amajor contribution to fibrotic kidney disease. Endoglin is a membrane glycoproteinof the TGF-alpha1 receptor system. The aim of this work was to determine the timecourseexpression of renal type I and IV collagens, endoglin and TGF-alpha1 in a ratmodel of induced tubulointerstitial fibrosis at 1, 3, 10 and 17 days after unilateralureteral obstruction (UUO). In 17 days-ligated (L)-renal samples, a marked interstitialfibrosis was detected by Massons trichromic and Sirius red staining, accompaniedby an increase in type I collagen expression as shown by immunohistochemicalanalysis. Northern blot studies revealed a progressive increase in collagen alpha2(I),TGF-alpha1 and endoglin mRNA expression in L kidneys when compared with the correspondingnon-ligated (NL) kidneys from the animals subjected to left UUO. Seventeendays after UUO, significant increases in collagen alpha2(I), collagen alpha1(IV),TGF-alpha1 and endoglin mRNA levels were detected in L kidneys vs NL kidneys. Significantlyhigher levels of the protein endoglin were found in L kidneys than in NLkidneys 10 and 17 days following obstruction. A marked increase expression forendoglin and TGF-alpha1 was localized in renal interstitium by immunohistochemical studies 17 days after obstruction. In conclusion, this work reports the upregulationof endoglin coincident to that of its ligand TGF-alpha1 in the kidneys of rats with progressivetubulointerstitial fibrosis induced by UUO (AU)
No disponible
Subject(s)
Male , Rats , Animals , Renal Insufficiency, Chronic/physiopathology , Glycoproteins , Urethral Obstruction/physiopathology , Transforming Growth Factor beta , Rats, Wistar/physiology , Fibrosis/physiopathology , Nephritis, Interstitial/physiopathologyABSTRACT
Estudios previos han demostrado que la inhibición aguda de la síntesis de óxidonítrico (NO) mejora la excreción de agua y sodio y la hipotensión arterial en ratascirróticas con. En este trabajo hemos analizado los efectos renales producidos por el tratamiento crónico (10 días) con aminoguanidina (AG, 100 mg/kg/día), un inhibidor preferente de la sin tasa inducible de NO (iNOS), o con Nw-Nitro-L-Arginina Methyl Ester (L-NAME, 0,5 mg/kg/día), un inhibidor no selectivo de la sintasa de NO, en un modelo experimental de cirrosis hepática y ascitis en ratas (inhalación de tetracloruro de carbono). Las ratas cirróticas no tratadas tenían menor presión arterial media (PAM), diuresis, natriuresis y tasa de filtración glomerular(TFG) y similar flujo sanguíneo renal (FSR) que sus controles. La administración crónica de AG no modificó ninguno de esos parámetros ni en las controles ni en las cirróticas. Sin embargo, el tratamiento crónico con L-NAME normalizó la PAM y aumentó significativamente la diuresis y natriuresis de los animales cirróticos, mientras que en los animales controles los efectos no fueron significativos. Estosdatos indican que la inhibición crónica de la síntesis de óxido nítrico con (..) (AU)
Previous studies have shown that acute inhibition of nitric oxide (NO) synthesis improves sodium and water excretion and increases blood pressure in cirrhotic rats with ascites, thus suggesting that NO is an important factor contributing to the arterial hypotension and sodium retention of liver cirrhosis. In the present work we have analyzed the renal effects derived from the chronic oral treatment(10 days) with amino guanidine (AG, 100 mg/kg/day), a preferential inhibitor of inducible NO synthase (iNOS), or Nw-Nitro-L-Arginine Methyl Ester (L-NAME, 0.5mg/kg/day), a nonselective inhibitor of NOS, in an experimental model of livercirrhosis with ascites (carbon tetrachloride inhalation). Untreated cirrhotic rats showed lower mean arterial pressure (MAP), diuresis, natriuresis and glomerular filtration rate (GFR) and similar renal blood flow (RBF) compared with the (..) (AU)
