ABSTRACT
The success of reproductive management in the sheep industry depends on several factors. Among them, early pregnancy diagnosis stands to improve the rates of exploration of sheep livestock, allowing the identification of fertility problems, disposal of infertile animals, food supplementation of pregnant females and maximizing production and profits. Therefore, this work aimed to develop an Enzyme Linked Immunosorbent Assay (ELISA) for detection of progesterone in peripheral blood samples from sheep of Santa Inês breed. A calibration curve was made on the test plate at which a fraction of 100 L of diluted antigen was deposited in the wells. The antigen (P4) was diluted sample in methanol/water and incubated at 37ºC for one hour; then 200 L were deposited from the PBS-BSA 1% and was followed by incubation overnight at 10ºC. 100 L of anti-P4 serum produced in rabbits were added and another incubation at 37ºC for one hour was performed, followed by two washes with PBS-T-G. Then it was added 100 L of the conjugate anti rabbit peroxidase labeled, followed by another incubation at 37ºC for one hour; two washing were done with PBS-T-G and drying the plate; revelation with TMB (Sure Blue) was performed using 100 L of Sure Blue for 10 minutes and the reaction stopped, the plate was read at 595 nm; another revelation was done with PNPP and the plate reading was done at 450 nm. The r
O artigo não possui resumo em português.
ABSTRACT
Onfaloflebitis is common in lambs born in poor hygienic conditions and occurs where inadequate treatment of umbilical healing is placed. Males are more affected lambs probably because of contact with urine that slows healing umbilical and removes some medications or topical antibiotics applied facilitating the bacteria entrance, mainly Fusobacterium necrophorum affecting joints, lungs, kidneys, liver, leading to animal death. Platelets are blood cells that interact with several receptors and modulate platelet function. It promotes hemostasis through four mechanisms: adhesion, release of platelet secretion, aggregation and pro-coagulation action. Complex network of growth factors are involved in tissue homeostasis such as transforming growth factor (TGF)-b, platelet-derived growth factor (PDGF), insulin-like growth factor (IGF), fibroblast growth factor (FGF)-b and hepatocyte growth factor (HGF). These factors and other bioactive molecules are contained in platelet alpha granules, and the use of intra-articular injections of platelet concentrate has been proposed as a minimally invasive solution to promote cartilage, osteo and muscular healing. Its use in regenerative medicine represents a simple, low-cost, and minimally invasive approach that might fasten healing processes. Regenerative treatment with autologous products, including the PRP (Platelet Rich Plasma) have been widel
O artigo não apresenta resumo em português.
ABSTRACT
Onfaloflebitis is common in lambs born in poor hygienic conditions and occurs where inadequate treatment of umbilical healing is placed. Males are more affected lambs probably because of contact with urine that slows healing umbilical and removes some medications or topical antibiotics applied facilitating the bacteria entrance, mainly Fusobacterium necrophorum affecting joints, lungs, kidneys, liver, leading to animal death. Platelets are blood cells that interact with several receptors and modulate platelet function. It promotes hemostasis through four mechanisms: adhesion, release of platelet secretion, aggregation and pro-coagulation action. Complex network of growth factors are involved in tissue homeostasis such as transforming growth factor (TGF)-b, platelet-derived growth factor (PDGF), insulin-like growth factor (IGF), fibroblast growth factor (FGF)-b and hepatocyte growth factor (HGF). These factors and other bioactive molecules are contained in platelet alpha granules, and the use of intra-articular injections of platelet concentrate has been proposed as a minimally invasive solution to promote cartilage, osteo and muscular healing. Its use in regenerative medicine represents a simple, low-cost, and minimally invasive approach that might fasten healing processes. Regenerative treatment with autologous products, including the PRP (Platelet Rich Plasma) have been widel
O artigo não apresenta resumo em português.
ABSTRACT
The success of reproductive management in the sheep industry depends on several factors. Among them, early pregnancy diagnosis stands to improve the rates of exploration of sheep livestock, allowing the identification of fertility problems, disposal of infertile animals, food supplementation of pregnant females and maximizing production and profits. Therefore, this work aimed to develop an Enzyme Linked Immunosorbent Assay (ELISA) for detection of progesterone in peripheral blood samples from sheep of Santa Inês breed. A calibration curve was made on the test plate at which a fraction of 100 L of diluted antigen was deposited in the wells. The antigen (P4) was diluted sample in methanol/water and incubated at 37ºC for one hour; then 200 L were deposited from the PBS-BSA 1% and was followed by incubation overnight at 10ºC. 100 L of anti-P4 serum produced in rabbits were added and another incubation at 37ºC for one hour was performed, followed by two washes with PBS-T-G. Then it was added 100 L of the conjugate anti rabbit peroxidase labeled, followed by another incubation at 37ºC for one hour; two washing were done with PBS-T-G and drying the plate; revelation with TMB (Sure Blue) was performed using 100 L of Sure Blue for 10 minutes and the reaction stopped, the plate was read at 595 nm; another revelation was done with PNPP and the plate reading was done at 450 nm. The r
O artigo não possui resumo em português.