Heterogeneity of linalool chemotypes of Lippia alba (Mill.) N.E.Br., based on clonal half-sib progenies

Lippia alba (Mill.) N.E.Br. is an aromatic and medicinal shrub native to the American continent. Despite its potential as a source of essential oil for the pharmaceutical and cosmetics industries, few selection and genetic improvement studies have been carried out. The aim of this study was to provide genetic information on this species for breeding programs, showing its selection potential, by investigating clonal half-sib progenies. The following characteristics were evaluated per plant: leaf dry mass (LDM), total dry mass (TDM), leaf yield (LY), essential oil yield (EOY) and oil production (OP). Estimates were made for the several genetic parameters including absolute genetic gain at 30% selection intensity, correlations and relative contribution of additive and environmental effects to phenotypic correlation. Two experimental trials on 30 progenies were conducted: one in Campinas, state of São Paulo (SP), Brazil, with two harvests of the aerial part, and one in Monte Alegre do Sul, SP, Brazil, with only one harvest. The trials were conducted in a randomized block design consisting of subplots with three replications, each plot (progeny) consisting of 8 to 15 clonally-replicated plants with subplot harvesting. Variations were detected between progenies and harvests, as well as progeny/harvest interactions in the split plot experiment. High heritability and genetic gains were obtained at both sites for LDM, TDM and OP. The lowest variations among progenies were obtained for LY and EOY, highlighting selection problems. Negative additive genetic correlations were obtained for EOY × LDM, EOY × TDM, LY × TDM and LY × LDM. Selection for LDM resulted in increased oil production per plant (OP), even where there was a negative correlation between LDM × EOY.

Heterogeneity of linalool chemotypes of Lippia alba (Mill.) N.E.Br., based on clonal half-sib progenies

Lippia alba (Mill.) N.E.Br. is an aromatic and medicinal shrub native to the American continent. Despite its potential as a source of essential oil for the pharmaceutical and cosmetics industries, few selection and genetic improvement studies have been carried out. The aim of this study was to provide genetic information on this species for breeding programs, showing its selection potential, by investigating clonal half-sib progenies. The following characteristics were evaluated per plant: leaf dry mass (LDM), total dry mass (TDM), leaf yield (LY), essential oil yield (EOY) and oil production (OP). Estimates were made for the several genetic parameters including absolute genetic gain at 30% selection intensity, correlations and relative contribution of additive and environmental effects to phenotypic correlation. Two experimental trials on 30 progenies were conducted: one in Campinas, state of São Paulo (SP), Brazil, with two harvests of the aerial part, and one in Monte Alegre do Sul, SP, Brazil, with only one harvest. The trials were conducted in a randomized block design consisting of subplots with three replications, each plot (progeny) consisting of 8 to 15 clonally-replicated plants with subplot harvesting. Variations were detected between progenies and harvests, as well as progeny/harvest interactions in the split plot experiment. High heritability and genetic gains were obtained at both sites for LDM, TDM and OP. The lowest variations among progenies were obtained for LY and EOY, highlighting selection problems. Negative additive genetic correlations were obtained for EOY × LDM, EOY × TDM, LY × TDM and LY × LDM. Selection for LDM resulted in increased oil production per plant (OP), even where there was a negative correlation between LDM × EOY.

Performance of ginger grass (Lippia alba) for traits related to the production of essential oil

Lippia alba (Verbenaceae) is a shrub whose essential oil has important biological, pharmacological, and aromatizing properties. To reach the sustained cultivation of new species with economic potential, the present study aimed to evaluate L. alba performance for fresh leaf matter (FM), leaf dry matter (DM), virus symptoms (VS - Cucumber mosaic virus, CMV), oil yield (OY), and oil chemical composition (OC), and to evaluate DM stability and adaptability. Ten genotypes of four chemical groups (chemotypes) were evaluated in six experiments designed as randomized blocks with two plants per plot, over the whole State of São Paulo, Brazil. A 2.0 x 0.4 x 1.6 m spacing was used in Monte Alegre do Sul and Pindorama, while a 1.0 x 0.4 x 0.6 m spacing was used in Campinas, where four experiments were established under different irrigation and fertilization conditions. The genotype effect was significant (p 0.05) for all traits evaluated, with high leaf productivity of IAC-16 (citral chemotype), best OY means in the linalool and limonene/carvone chemotypes, and susceptibility of the latter chemotype to CMV. The genotype performance oscillations in the six environments were significant for FM and DM, and despite their significance for phytochemical traits (OY and OC), they were of low magnitude. No qualitative variation was detected for OC. The IAC-2 (linalool) and IAC-13 (limonene/carvone) genotypes showed high stability and wide adaptability, and are recommended to establish initial cultivations of this species. This research also indicated genetic sources to start Lippia alba genetic breeding programs.

A Lippia alba (Verbenaceae) é um arbusto produtor de óleo essencial com importantes atividades biológicas, farmacológicas e aromatizantes. Visando o cultivo sustentado de uma nova espécie com potencial econômico, os objetivos deste trabalho foram avaliar o desempenho da L. alba para massa de folha fresca (MF), massa de folha seca (MS), sintomas de vírus (SV - Cucumber mosaic virus, CMV), rendimento de óleo essencial (RE) e composição química do óleo (CQ), e avaliar a estabilidade e adaptabilidade fenotípica para o caráter MS. Dez genótipos pertencentes a quatro grupos químicos foram testados em seis experimentos instalados no estado de São Paulo, Brasil, com delineamento em blocos ao acaso e duas plantas por parcela. Em Monte Alegre do Sul e Pindorama, foi utilizado espaçamento de 2,0 x 0,4 x 1,6 m e em Campinas, foram instalados quatro experimentos 1,0 x 0,4 x 0,6 m sob condições distintas de adubação e irrigação. O efeito de genótipo foi significativo para todas as características avaliadas, revelando elevada produtividade de folhas do IAC-16 (citral), RE Yamamoto et al. superior dos quimiotipos linalol e limoneno/carvona e susceptibilidade deste último ao CMV. As oscilações no desempenho dos genótipos nos diferentes experimentos foram de baixa magnitude para os caracteres fitoquímicos (RE e CQ) e nenhuma variação qualitativa foi observada para CQ. Os genótipos IAC-2 (linalol) e IAC-13 (limoneno/carvona) apresentaram-se estáveis para MS e com ampla adaptabilidade, e são recomendados para o início do cultivo da espécie. A presente pesquisa fornece subsídios também para o início de programas de melhoramento genético em L. alba.

Performance of ginger grass (Lippia alba) for traits related to the production of essential oil

Lippia alba (Verbenaceae) is a shrub whose essential oil has important biological, pharmacological, and aromatizing properties. To reach the sustained cultivation of new species with economic potential, the present study aimed to evaluate L. alba performance for fresh leaf matter (FM), leaf dry matter (DM), virus symptoms (VS - Cucumber mosaic virus, CMV), oil yield (OY), and oil chemical composition (OC), and to evaluate DM stability and adaptability. Ten genotypes of four chemical groups (chemotypes) were evaluated in six experiments designed as randomized blocks with two plants per plot, over the whole State of São Paulo, Brazil. A 2.0 x 0.4 x 1.6 m spacing was used in Monte Alegre do Sul and Pindorama, while a 1.0 x 0.4 x 0.6 m spacing was used in Campinas, where four experiments were established under different irrigation and fertilization conditions. The genotype effect was significant (p 0.05) for all traits evaluated, with high leaf productivity of IAC-16 (citral chemotype), best OY means in the linalool and limonene/carvone chemotypes, and susceptibility of the latter chemotype to CMV. The genotype performance oscillations in the six environments were significant for FM and DM, and despite their significance for phytochemical traits (OY and OC), they were of low magnitude. No qualitative variation was detected for OC. The IAC-2 (linalool) and IAC-13 (limonene/carvone) genotypes showed high stability and wide adaptability, and are recommended to establish initial cultivations of this species. This research also indicated genetic sources to start Lippia alba genetic breeding programs.

A Lippia alba (Verbenaceae) é um arbusto produtor de óleo essencial com importantes atividades biológicas, farmacológicas e aromatizantes. Visando o cultivo sustentado de uma nova espécie com potencial econômico, os objetivos deste trabalho foram avaliar o desempenho da L. alba para massa de folha fresca (MF), massa de folha seca (MS), sintomas de vírus (SV - Cucumber mosaic virus, CMV), rendimento de óleo essencial (RE) e composição química do óleo (CQ), e avaliar a estabilidade e adaptabilidade fenotípica para o caráter MS. Dez genótipos pertencentes a quatro grupos químicos foram testados em seis experimentos instalados no estado de São Paulo, Brasil, com delineamento em blocos ao acaso e duas plantas por parcela. Em Monte Alegre do Sul e Pindorama, foi utilizado espaçamento de 2,0 x 0,4 x 1,6 m e em Campinas, foram instalados quatro experimentos 1,0 x 0,4 x 0,6 m sob condições distintas de adubação e irrigação. O efeito de genótipo foi significativo para todas as características avaliadas, revelando elevada produtividade de folhas do IAC-16 (citral), RE Yamamoto et al. superior dos quimiotipos linalol e limoneno/carvona e susceptibilidade deste último ao CMV. As oscilações no desempenho dos genótipos nos diferentes experimentos foram de baixa magnitude para os caracteres fitoquímicos (RE e CQ) e nenhuma variação qualitativa foi observada para CQ. Os genótipos IAC-2 (linalol) e IAC-13 (limoneno/carvona) apresentaram-se estáveis para MS e com ampla adaptabilidade, e são recomendados para o início do cultivo da espécie. A presente pesquisa fornece subsídios também para o início de programas de melhoramento genético em L. alba.

Stock-scion interactions on growth and rubber yield of Hevea brasiliensis

Rubber tree cultivation [Hevea brasiliensis (Willd. ex Adr. de Juss.) Muell.-Arg.] uses mostly the budding propagation method, however the expected stand uniformity due to the use of vegetative propagation is theoretical, since high variation for yield and vigor is caused by rootstock influence. The objective of this study was to assess several combinations between clonal rootstocks and clone scions through diallel analysis, aiming at high productivity and vigor. Data consisted of five years of dry rubber yield and girth at panel opening from an experiment using a split-plot design with four replications, conducted at Pindorama, SP, Brazil, considering six rootstocks (GT 1, IAN 873, PB 235, RRIM 600, RRIM 701 and unselected seedlings) and six scions (GT 1, IAN 873, PB 235, PR 107, RRIM 600 and RRIM 701). A 6 FONT FACE=Symbol>´ /FONT> 6 partial diallel analysis summing up 36 combinations was undertaken, and general compatibility for each group of materials (rootstocks and scions) and specific compatibility among all combinations were estimated. The results have shown that PB 235 and IAN 873 rootstocks provided high yields and general compatibility values (7.9348 and 2.7647, respectively). The use of unselected seedlings as rootstocks is not recommended because they presented very low compatibility values for dry rubber yield. Towards growth vigor, the tested rootstocks have shown similar performance.

Na cultura da seringueira [Hevea brasiliensis (Willd. ex Adr. de Juss.) Muell.-Arg.], o método de propagação mais usado é a enxertia, mas a uniformidade esperada usando propagação vegetativa é teórica, uma vez que altas variações para produção e vigor são causados pela influência do porta-enxerto. O objetivo do presente trabalho foi avaliar várias combinações entre porta-enxertos clonais e enxertos (clones) através de análise dialélica, visando-se alta produção e vigor. Os resultados constaram de cinco anos de produção de borracha seca e perímetro de caule na abertura do painel de um experimento em parcelas subdivididas com quatro repetições, conduzido em Pindorama, SP, Brasil, considerando-se seis porta-enxertos (GT 1, IAN 873, PB 235, RRIM 600, RRIM 701 e sementes não-selecionadas UnS) e seis enxertos (GT 1, IAN 873, PB 235, PR 107, RRIM 600 e RRIM 701). Foi realizada a análise do dialelo parcial 6 FONT FACE=Symbol>´ /FONT> 6, somando 36 combinações, e Compatibilidade Geral (GC) para cada grupo de materiais (porta-enxertos e enxertos) e Compatibilidade Específica (SC) dentre todas as combinações foram estimadas. Os resultados mostararam que os porta-enxertos têm forte efeito sobre a produção de borracha seca, e levaram à recomendação do PB 235 e do IAN 873 como escolhas seguras de porta-enxertos para as condições do Planalto Paulista, sendo que proporcionaram altas produções e altos valores de Compatibilidade Geral (7.9348 e 2.7647, respectivamente). O uso de sementes não-selecionadas (UnS) como porta-enxertos não é recomendado, pois esse material apresentou valores muito baixos de compatibilidade em relação à produção de borracha seca. Em relação ao vigor de crescimento, os porta-enxertos testados mostraram desempenho semelhante.

Stock-scion interactions on growth and rubber yield of Hevea brasiliensis

Rubber tree cultivation [Hevea brasiliensis (Willd. ex Adr. de Juss.) Muell.-Arg.] uses mostly the budding propagation method, however the expected stand uniformity due to the use of vegetative propagation is theoretical, since high variation for yield and vigor is caused by rootstock influence. The objective of this study was to assess several combinations between clonal rootstocks and clone scions through diallel analysis, aiming at high productivity and vigor. Data consisted of five years of dry rubber yield and girth at panel opening from an experiment using a split-plot design with four replications, conducted at Pindorama, SP, Brazil, considering six rootstocks (GT 1, IAN 873, PB 235, RRIM 600, RRIM 701 and unselected seedlings) and six scions (GT 1, IAN 873, PB 235, PR 107, RRIM 600 and RRIM 701). A 6 FONT FACE=Symbol>´ /FONT> 6 partial diallel analysis summing up 36 combinations was undertaken, and general compatibility for each group of materials (rootstocks and scions) and specific compatibility among all combinations were estimated. The results have shown that PB 235 and IAN 873 rootstocks provided high yields and general compatibility values (7.9348 and 2.7647, respectively). The use of unselected seedlings as rootstocks is not recommended because they presented very low compatibility values for dry rubber yield. Towards growth vigor, the tested rootstocks have shown similar performance.

Na cultura da seringueira [Hevea brasiliensis (Willd. ex Adr. de Juss.) Muell.-Arg.], o método de propagação mais usado é a enxertia, mas a uniformidade esperada usando propagação vegetativa é teórica, uma vez que altas variações para produção e vigor são causados pela influência do porta-enxerto. O objetivo do presente trabalho foi avaliar várias combinações entre porta-enxertos clonais e enxertos (clones) através de análise dialélica, visando-se alta produção e vigor. Os resultados constaram de cinco anos de produção de borracha seca e perímetro de caule na abertura do painel de um experimento em parcelas subdivididas com quatro repetições, conduzido em Pindorama, SP, Brasil, considerando-se seis porta-enxertos (GT 1, IAN 873, PB 235, RRIM 600, RRIM 701 e sementes não-selecionadas UnS) e seis enxertos (GT 1, IAN 873, PB 235, PR 107, RRIM 600 e RRIM 701). Foi realizada a análise do dialelo parcial 6 FONT FACE=Symbol>´ /FONT> 6, somando 36 combinações, e Compatibilidade Geral (GC) para cada grupo de materiais (porta-enxertos e enxertos) e Compatibilidade Específica (SC) dentre todas as combinações foram estimadas. Os resultados mostararam que os porta-enxertos têm forte efeito sobre a produção de borracha seca, e levaram à recomendação do PB 235 e do IAN 873 como escolhas seguras de porta-enxertos para as condições do Planalto Paulista, sendo que proporcionaram altas produções e altos valores de Compatibilidade Geral (7.9348 e 2.7647, respectivamente). O uso de sementes não-selecionadas (UnS) como porta-enxertos não é recomendado, pois esse material apresentou valores muito baixos de compatibilidade em relação à produção de borracha seca. Em relação ao vigor de crescimento, os porta-enxertos testados mostraram desempenho semelhante.