ABSTRACT
In the present study, comparative proteomic analysis was performed in rats subjected to water immersionrestraint stress (WRS). A total of 26 proteins were differentially expressed and identified using matrixassisted laser desorption/ionization time of flight mass spectrometry. Among the 26 differentially expressed protein spots identified, 13 proteins were significantly upregulated under WRS, including pyruvate kinase and calreticulin, which may be closely associated with energy metabolism. In addition, 12 proteins were downregulated under WRS, including hemoglobin subunit ß2 and keratin type II cytoskeletal 8, which may be important in protein metabolism and cell death. Gene Ontology analysis revealed the cellular distribution, molecular function and biological processes of the identified proteins. The mRNA levels of certain differentially expressed proteins were analyzed using fluorescence quantitative polymerase chain reaction analysis. The results of the present study aimed to offer insights into proteins, which are differentially expressed in gastric ulcers in stress, and provide theoretical evidence of a radical cure for gastric ulcers in humans.
Subject(s)
Proteome , Proteomics , Restraint, Physical , Stomach Ulcer/etiology , Stomach Ulcer/metabolism , Stress, Physiological , Animals , Computational Biology/methods , Databases, Genetic , Disease Models, Animal , Electrophoresis, Gel, Two-Dimensional , Gastric Mucosa/metabolism , Gastric Mucosa/pathology , Gene Ontology , Male , Molecular Sequence Annotation , Proteomics/methods , Rats , Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization , Stomach Ulcer/pathologyABSTRACT
OBJECTIVE: To investigate the relation of liver mass to, liver hymodynamics and soluble intercellular adhesion molecule-1 in patients with liver cirrhosis. METHODS: The liver mass was measured by liver biopsy, hepatic hemodynamics by ultrasonography, and the contents of hyalurionic acid (HA), human procollagen III (HPC III) and collagen type IV (C IV) by radioimmunoassay in 100 patients with liver cirrhosis in compensation stage and 30 normal control subjects. RESULTS: Greater liver mass was accompanied by more severe liver fibrosis and reduced liver blood flow. The contents of HA, HPC III and C IV were obvious higher in the patients than in the normal controls (P<0.05) and increased with the liver mass. The liver mass was positively related to serum liver fibrosis indices (r=0.5612, P<0.05). CONCLUSION: Liver mass in patients with liver cirrhosis is related to hepatic hemodynamics, indices for liver fibrosis and liver pathology.
