ABSTRACT
In this study, we report the identification of HLA-B*1314 that differs from B*130201 at two single nucleotide positions.
Subject(s)
HLA-B Antigens/genetics , Alleles , Amino Acid Sequence , Amino Acid Substitution/genetics , Base Sequence , HLA-B13 Antigen , Haplotypes , Histocompatibility Antigens Class I/genetics , Histocompatibility Testing , Humans , Living Donors , Molecular Sequence Data , Oligonucleotides/genetics , Polymerase Chain Reaction , Sequence Analysis, DNAABSTRACT
Lupus panniculitis is a rare variant of cutaneous lupus erythematosus. Two typical cases demonstrate the association of lupus panniculitis with signs of discoid lupus erythematosus and systemic lupus erythematosus. Clinically it is characterised by subcutaneous nodules or plaques, which may ulcerate leaving depressed atrophic scars. Histologically the lesions show lobular panniculitis. A positive immunofluorescent band test with deposition of IgG, IgM and C3 at the dermal-epidermal junction and blood vessel walls helps to confirm the diagnosis. The knowledge of the clinical features and histopathology of the disease is important because lupus panniculitis may precede systemic lupus erythematosus by some years.
Subject(s)
Panniculitis, Lupus Erythematosus/diagnosis , Adipose Tissue/pathology , Azathioprine/administration & dosage , Basement Membrane/pathology , Complement C3/analysis , Connective Tissue/pathology , Drug Therapy, Combination , Female , Fibrosis , Fluorescent Antibody Technique , Glucocorticoids/administration & dosage , Humans , Immunoglobulin G/analysis , Immunoglobulin M/analysis , Middle Aged , Panniculitis, Lupus Erythematosus/drug therapy , Panniculitis, Lupus Erythematosus/pathology , Skin/pathologyABSTRACT
Cutaneous metastasis from gastric carcinoma is uncommon. We describe a patient with a metastasis from gastric carcinoma to a congenital melanocytic nevus. The diagnosis was confirmed by positive immunohistochemical staining for cytokeratin 20 and lack of cytokeratin 7.
Subject(s)
Carcinoma/secondary , Neoplasms, Multiple Primary/pathology , Nevus, Pigmented/congenital , Nevus, Pigmented/pathology , Skin Neoplasms/congenital , Skin Neoplasms/secondary , Stomach Neoplasms/pathology , Carcinoma/pathology , Collagen , Cytoplasm/ultrastructure , Female , Hair/pathology , Humans , Keratins/analysis , Melanocytes/pathology , Middle Aged , S100 Proteins/analysis , Skin Neoplasms/pathology , Sweat Glands/pathology , Vimentin/analysisABSTRACT
A murine monoclonal antibody (called H-11) that binds to the p 24 core protein of HIV-1 was characterized by radioimmuno-precipitation, immunofluorescence, western blot assays, immunocytochemistry and immunohistochemistry. This antibody was found to be especially suited for demonstrating the presence of HIV-1 in formalin-fixed, paraffin-embedded tissues.
