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1.
Future Microbiol ; 15: 21-33, 2020 01.
Article in English | MEDLINE | ID: mdl-32043361

ABSTRACT

Aim: This study aimed to evaluate the activity of 2'-hydroxychalcone-loaded in nanoemulsion (NLS + 2'chalc), the cytotoxic effect and toxicity against Paracoccidioides brasiliensis and Paracoccidioides lutzii using a zebrafish model. Materials & methods: Preparation and physical-chemical characterization of nanoemulsion (NLS) and NLS + 2'chalc were performed. MIC and minimum fungicide concentration, cytotoxicity and toxicity were also evaluated in the Danio rerio model. Results: NLS + 2'chalc showed fungicidal activity against Paracoccidioides spp. without cytotoxicity in MRC5 and HepG2 lines. It also had high selectivity index values and no toxicity in the zebrafish model based on MIC values. Conclusion: NLS + 2'chalc is a potential new alternative treatment for paracoccidioidomycosis.


Subject(s)
Antifungal Agents/pharmacology , Chalcones/pharmacology , Paracoccidioides/drug effects , Animals , Cell Line , Chalcones/chemistry , Emulsions/pharmacology , Fibroblasts/drug effects , Hep G2 Cells , Humans , Microbial Sensitivity Tests , Models, Animal , Nanoparticles , Paracoccidioidomycosis/microbiology , Zebrafish
2.
Future Microbiol ; 14: 1087-1097, 2019 09.
Article in English | MEDLINE | ID: mdl-31512522

ABSTRACT

Aim: In this work, mastoparan analog peptides from wasp venom were tested against Candida albicans and safety assays were performed using cell culture and model zebrafish. Materials & methods: Minimal inhibitory concentration was determined and toxicity was performed using human skin keratinocyte and embryo zebrafish. Also, permeation of peptides through embryo chorion was performed. Results: The peptides demonstrated anti-C. albicans activity, with low cytotoxicity and nonteratogenicity in Danio rerio. The compounds had different permeation through chorion, suggesting that this occurs due to modifications in their amino acid sequence. Conclusion: The results showed that the studied peptides can be used as structural study models for novel potential antifungal agents.


Subject(s)
Antifungal Agents/pharmacology , Candida albicans/drug effects , Intercellular Signaling Peptides and Proteins/pharmacology , Peptides/pharmacology , Wasp Venoms/pharmacology , Animals , Antifungal Agents/administration & dosage , Antifungal Agents/adverse effects , Antifungal Agents/toxicity , Cell Survival/drug effects , Cells, Cultured , Drug-Related Side Effects and Adverse Reactions/pathology , Humans , Intercellular Signaling Peptides and Proteins/administration & dosage , Intercellular Signaling Peptides and Proteins/adverse effects , Intercellular Signaling Peptides and Proteins/toxicity , Keratinocytes/drug effects , Microbial Sensitivity Tests , Peptides/administration & dosage , Peptides/adverse effects , Peptides/toxicity , Wasp Venoms/administration & dosage , Wasp Venoms/adverse effects , Wasp Venoms/toxicity , Zebrafish
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