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1.
PLoS Genet ; 11(8): e1005440, 2015 Aug.
Article in English | MEDLINE | ID: mdl-26274446

ABSTRACT

In multicellular organisms, growth and proliferation is adjusted to nutritional conditions by a complex signaling network. The Insulin receptor/target of rapamycin (InR/TOR) signaling cascade plays a pivotal role in nutrient dependent growth regulation in Drosophila and mammals alike. Here we identify Cyclin G (CycG) as a regulator of growth and metabolism in Drosophila. CycG mutants have a reduced body size and weight and show signs of starvation accompanied by a disturbed fat metabolism. InR/TOR signaling activity is impaired in cycG mutants, combined with a reduced phosphorylation status of the kinase Akt1 and the downstream factors S6-kinase and eukaryotic translation initiation factor 4E binding protein (4E-BP). Moreover, the expression and accumulation of Drosophila insulin like peptides (dILPs) is disturbed in cycG mutant brains. Using a reporter assay, we show that the activity of one of the first effectors of InR signaling, Phosphoinositide 3-kinase (PI3K92E), is unaffected in cycG mutants. However, the metabolic defects and weight loss in cycG mutants were rescued by overexpression of Akt1 specifically in the fat body and by mutants in widerborst (wdb), the B'-subunit of the phosphatase PP2A, known to downregulate Akt1 by dephosphorylation. Together, our data suggest that CycG acts at the level of Akt1 to regulate growth and metabolism via PP2A in Drosophila.


Subject(s)
Cyclin G/physiology , Drosophila melanogaster/metabolism , Animals , Body Weight , Brain/metabolism , Drosophila Proteins/metabolism , Drosophila melanogaster/growth & development , Fat Body/metabolism , Inhibitor of Apoptosis Proteins/metabolism , Lipid Metabolism , Phosphoprotein Phosphatases/metabolism , Phosphorylation , Protein Binding , Protein Processing, Post-Translational , Proto-Oncogene Proteins c-akt/metabolism
2.
J Cell Sci ; 125(Pt 22): 5555-63, 2012 Nov 15.
Article in English | MEDLINE | ID: mdl-22976300

ABSTRACT

Cyclin G (CycG) belongs to the atypical cyclins, which have diverse cellular functions. The two mammalian CycG genes, CycG1 and CycG2, regulate the cell cycle in response to cell stress. Detailed analyses of the role of the single Drosophila cycG gene have been hampered by the lack of a mutant. We generated a null mutant in the Drosophila cycG gene that is female sterile and produces ventralised eggs. This phenotype is typical of the downregulation of epidermal growth factor receptor (EGFR) signalling during oogenesis. Ventralised eggs are also observed in mutants (for example, mutants of the spindle class) that are defective in meiotic DNA double-strand break repair. Double-strand breaks (DSBs) induce a meiotic checkpoint by activating Mei-41 kinase (the Drosophila ATR homologue), thereby indirectly causing dorsoventral patterning defects. We provide evidence for the role of CycG in meiotic checkpoint control. The increased incidence of DSBs in cycG mutant germaria may reflect inefficient DSB repair. Therefore, the downregulation of Mei-W68 (an endonuclease that induces meiotic DSBs), Mei-41, or Drosophila melanogaster Chk2 (a downstream kinase that initiates the meiotic checkpoint) rescues the cycG mutant eggshell phenotype. In vivo, CycG associates with Rad9 and BRCA2. These two proteins are components of the 9-1-1 complex, which is involved in sensing DSBs and in activating meiotic checkpoint control. Therefore, we propose that CycG has a role in an early step of meiotic recombination repair, thereby affecting EGFR-mediated patterning processes during oogenesis.


Subject(s)
Cyclin G/metabolism , Drosophila melanogaster/cytology , Drosophila melanogaster/genetics , Meiosis/genetics , Recombinational DNA Repair/genetics , Animals , Body Patterning/genetics , Cyclin G/genetics , DNA Breaks, Double-Stranded , Drosophila Proteins/genetics , Drosophila Proteins/metabolism , Drosophila melanogaster/embryology , Female , Gene Expression Regulation, Developmental , Immunoprecipitation , Male , Mutant Proteins/metabolism , Mutation/genetics , Oocytes/cytology , Oocytes/metabolism , Ovary/cytology , Ovary/metabolism , Oviposition/physiology , Ovum/metabolism , Protein Binding/genetics , Reproducibility of Results
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