ABSTRACT
A dynamic model has been developed for chromatographic separation of mixed electrolyte solutions with non-ionic nanoporous adsorbents. The thermodynamic equilibrium condition at the pore entrance is written in terms of mixing, electrostatic and size-exclusion effects. The model is tested against experimental data measured with three binary mixtures on hypercrosslinked polystyrene and nanoporous carbon. The selectivity of the nanoporous adsorbents can be explained by the size-exclusion of the electrolytes and enrichment of both electrolytes in frontal chromatographic runs can be correlated satisfactorily with the proposed model. The model is also used to demonstrate continuous separation in a simulated moving-bed (SMB) system.
Subject(s)
Chromatography, Gel/methods , Electrolytes/isolation & purification , Models, Theoretical , Nanotechnology , Adsorption , Electrolytes/chemistryABSTRACT
We have been able to assign the human catechol-O-methyltransferase gene (COMT) to chromosome 22q11.2 by using Southern blot analysis of panels of somatic cell hybrids and chromosomal in situ hybridization. Furthermore, Southern blot analysis of DNA from blood and bone marrow samples of a patient with chronic myeloid leukemia (CML), having an extra Philadelphia chromosome (Ph1) in addition to the one produced by the reciprocal translocation between chromosomes 9 and 22, showed increased COMT and BCR gene dosage as compared to DNAs originating from CML patients with only one Ph1 chromosome or from chromosomally normal individuals. Control hybridizations of the same blot with TCRG- and TCRA-specific probes showed corresponding signal intensities in all samples. A relatively frequent two-allele COMT gene RFLP (PIC = 0.37) was recognized in DNAs digested with BglI. Our gene mapping result is in concordance with that previously reported by Brahe et al. (1986), who used an autoradiozymogram assay on different somatic cell hybrids to map this gene to chromosome 22.