Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 2 de 2
Filter
Add more filters










Database
Language
Publication year range
1.
Ecotoxicology ; 20(8): 1890-9, 2011 Nov.
Article in English | MEDLINE | ID: mdl-21706406

ABSTRACT

The Atlantic Wood Industries Superfund site (AWI) on the Elizabeth River in Portsmouth, VA is heavily contaminated with polycyclic aromatic hydrocarbons (PAHs) from a wood treatment facility. Atlantic killifish, or mummichog (Fundulus heteroclitus), at this Superfund site are exposed to very high concentrations of several carcinogens. In this study, we measured PAH concentrations in both fish tissues and sediments. Concurrently, we assessed different aspects of genotoxicity in the killifish exposed in situ. Both sediment and tissue PAH levels were significantly higher in AWI samples, relative to a reference site, but the chemistry profile was different between sediments and tissues. Killifish at AWI exhibited higher levels of DNA damage compared to reference fish, as measured via the flow cytometric method (FCM), and the damage was consistent with sediment PAH concentrations. Covalent binding of benzo[a]pyrene (BaP) metabolites to DNA, as measured via LC-MS/MS adduct detection methods, were also elevated and could be partially responsible for the DNA damage. Using similar LC-MS/MS methods, we found no evidence that oxidative DNA adducts had a role in observed genotoxicity.


Subject(s)
Fundulidae/genetics , Geologic Sediments/chemistry , Polycyclic Aromatic Hydrocarbons/analysis , Polycyclic Aromatic Hydrocarbons/toxicity , Water Pollutants, Chemical/toxicity , Animals , Benzo(a)pyrene/metabolism , Chromatography, Liquid , DNA Adducts/analysis , DNA Damage , Flow Cytometry/methods , Mutagenicity Tests , Rivers , Tandem Mass Spectrometry , Toxicity Tests, Chronic , Virginia , Water Pollution, Chemical
2.
Ecotoxicology ; 19(1): 185-95, 2010 Jan.
Article in English | MEDLINE | ID: mdl-19728085

ABSTRACT

Nanoparticles are being used in many commercial applications. We describe the toxicity of two commercial silver (Ag) nanoparticle (NP) products, NanoAmor and Sigma on Pimephales promelas embryos. Embryos were exposed to varying concentrations of either sonicated or stirred NP solutions for 96 h. LC(50) values for NanoAmor and Sigma Ag NPs were 9.4 and 10.6 mg/L for stirred and 1.25 and 1.36 mg/L for sonicated NPs, respectively. Uptake of Ag NPs into the embryos was observed after 24 h using Transmission Electron Microscopy and Ag NPs induced a concentration-dependent increase in larval abnormalities, mostly edema. Dissolved Ag released from Ag NPs was measured using Inductively Coupled-Mass Spectrometry and the effects tested were found to be three times less toxic when compared to Ag nitrate (AgNO(3)). The percentage of dissolved Ag released was inversely proportional to the concentration of Ag NPs with the lowest (0.625 mg/L) and highest (20 mg/L) concentrations tested releasing 3.7 and 0.45% dissolved Ag, respectively and percent release was similar regardless if concentrations were stirred or sonicated. Thus increased toxicity after sonication cannot be solely explained by dissolved Ag. We conclude that both dissolved and particulate forms of Ag elicited toxicity to fish embryos.


Subject(s)
Cyprinidae/metabolism , Embryo, Nonmammalian/drug effects , Nanoparticles/toxicity , Silver/toxicity , Analysis of Variance , Animals , Embryo, Nonmammalian/ultrastructure , Lethal Dose 50 , Mass Spectrometry , Microscopy, Electron, Transmission , Toxicity Tests
SELECTION OF CITATIONS
SEARCH DETAIL
...