Assessing the sterility and quality of gamma-irradiated pepper weevils, Anthonomus eugenii (Coleoptera: Curculionidae), toward the development of the sterile insect technique.

BACKGROUND: The pepper weevil (PW), Anthonomus eugenii, is an economically significant pest of cultivated Capsicum spp. pepper crops in North America where it remains a challenge to manage because of its cryptic immature life stages. The sterile insect technique (SIT) is a genetic pest management tactic that relies on the release of insects that have been sterilized with ionizing radiation to lower the population reproductive rate. Toward developing an effective PW-SIT program, this study has, for the first time, investigated the effects of gamma irradiation on the sterility and survival of this species. RESULTS: Among the array of doses tested, we found that pupal PW males and females irradiated at 110 Gy produced no adult offspring. Furthermore, females mated with a male irradiated at 110 Gy had high egg sterility (97.3%), and irradiated females nearly completely failed to lay eggs (97.5%). Individuals irradiated at this dose had a shortened lifespan (lethal time to 50% mortality values of 12 and 11 days for males and females, respectively) and quantitatively reduced spontaneous flight activity. The eclosion rate of PW pupae was not significantly reduced by any radiation treatment. CONCLUSION: This study suggests that PWs irradiated at a gamma radiation dose of 110 Gy as pupae could feasibly be used in a PW-SIT program, because both males and females were 100% sterile at this dose. These findings will inform the development of a SIT program that could considerably improve the sustainability and effectiveness of PW management in greenhouse and field pepper crops worldwide. © 2023 His Majesty the King in Right of Canada and The Authors. Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry. Reproduced with the permission of the Minister of Agriculture and Agri Food Canada.

Population Structure and Genetic Diversity of the Pepper Weevil (Coleoptera: Curculionidae) Using the COI Barcoding Region.

The pepper weevil Anthonomus eugenii Cano (Coleoptera: Curculionidae) is a pest of economic importance for Capsicum species pepper in North America that attacks the reproductive structures of the plant. The insect is distributed across Mexico, the United States, and the Caribbean, and is occasionally found during the pepper growing season in southern Ontario, Canada. Continuous spread of the insect to new areas is partially the result of global pepper trade. Here, we describe the genetic diversity of the pepper weevil using the mitochondrial COI barcoding region across most of its geographic range. In this study, 44 (H1-H44) highly similar haplotypes were identified, the greatest number of haplotypes and haplotype diversity were observed among specimens from its native Mexico, followed by specimens from the United States. Unlike Mexico, a low haplotype diversity was found among specimens from Canada, the Dominican Republic, Italy, and the Netherlands. Out of these 44 haplotypes, 29 are reported for the first time. Haplotype diversity in the Canadian population suggests either multiple and continuous introductions of the pepper weevil into this area or a single introduction of genetically diverse individuals. We discuss the importance of such population genetic data in tailoring pepper weevil management programs, using Canada as an example.

Host utilization by the pepper weevil (Anthonomus eugenii): suitability, preference and offspring performance.

BACKGROUND: Host plant selection is a key factor affecting the survival, population establishment, and spread of herbivorous insect pests. The pepper weevil is one of the most important pests of cultivated pepper in North America with a capacity to rapidly expand its geographic range, in part through its ability to switch between cultivated and wild Solanaceous host plants. Towards a better management of this pest, this study examined metrics of pepper weevil host use including oviposition preference, development time, and successful offspring emergence on wild Solanaceous species and cultivated pepper cultivars. RESULTS: Pepper weevil successfully developed within fruit of several Solanaceous species including eastern black (Solanum ptycanthum) and climbing nightshade (S. dulcamara), in which development time was on average 4 days faster relative to Capsicum annuum cv. jalapeno peppers. Oviposition events occurred in all fruit types assessed and no strong host preference was detected among these. However, the number of emerged offspring was significantly lower than the number of oviposition events in C. chinense cv. habanero pepper fruit. CONCLUSION: Although not all nightshade species are suitable hosts for pepper weevil development, those permissive to offspring production do allow for faster development than in C. annuum peppers. While host preference was not detected among fruit types tested, low offspring emergence from fruit with high capsaicin content suggests a reduced ability of pepper weevil to tolerate high concentrations of this metabolite. These findings help elucidate the factors influencing pepper weevil bionomics, and their implications on pepper weevil management are discussed. Published 2021. This article is a U.S. Government work and is in the public domain in the USA.

Assessing New Tools for Management of the Pepper Weevil (Coleoptera: Curculionidae) in Greenhouse and Field Pepper Crops.

The pepper weevil, Anthonomus eugenii Cano, is an economically important pest of field and greenhouse pepper crops in North America. In this study, a series of insecticides covering a broad-spectrum of insecticidal modes of action were assessed for their potential in managing the pepper weevil under laboratory and greenhouse conditions. To accomplish this, laboratory mini-spray tower and greenhouse cage trials were conducted that evaluated the efficacy of 16 conventional, reduced-risk, and microbial insecticides. In laboratory trials, adult weevils were sprayed with insecticides, placed on treated leaves within a cup cage, and were monitored for their survival over 10 d. Of the 16 insecticides tested, 8 provided greater than 60% weevil control, a threshold considered necessary for including products in further greenhouse testing. In greenhouse trials, adult weevil mortality, bud and foliar damage, bud and fruit abortion, and subsequent weevil offspring emergence were measured following each of three weekly insecticide applications. The most efficacious insecticides included kaolin clay and mineral oil, which performed as well as the thiamethoxam-positive control, and incurred 70 and 55% of adult weevil mortality, respectively. Additionally, kaolin clay and mineral oil reduced offspring weevil emergence by 59 and 54%, respectively, compared with untreated controls. Despite the clear challenge that controlling this pest represents, this study has identified useful new tools for the integrated management of the pepper weevil, which may accelerate the rate at which these become available for use in greenhouse and field pepper production.

Comparison of Transeius montdorensis (Acari: Phytoseiidae) to Other Phytoseiid Mites for the Short-Season Suppression of Western Flower Thrips, Frankliniella occidentalis (Thysanoptera: Thripidae).

Under winter and early spring greenhouse growing conditions, suppression of thrips by predatory mites can vary considerably on a species basis. For certain mite species, shorter photoperiods, cooler temperatures, and lower vapor pressures translate to reductions in predation, oviposition, and survival. Therefore, predator species need to be assessed simultaneously to identify those most suitable for use under short-season conditions. In this study, laboratory trials were first conducted to compare rates of Frankliniella occidentalis (Pergande) thrips predation, and oviposition by the phytoseiid predator Transeius montdorensis (Schicha) under simulated summer and winter conditions. Transeius montdorensis consumed similar numbers of first instar thrips, and laid a similar number of eggs under both conditions. In short-season greenhouse cage trials, crop establishment and predatory capacity of T. montdorensis were compared to those for three other predatory mites: Amblyseius swirskii (Athias-Henriot) (Acari: Phytoseiidae), Amblydromalus limonicus (Garman & McGregor) (Acari: Phytoseiidae) and Neoseiulus cucumeris (Oudemans) (Acari: Phytoseiidae). Over 4-5-wk trials performed in early spring in 2014 and 2016, the number of T. montdorensis mites on pepper plants was either equal or greater to levels in other treatments. In T. montdorensis cages, high levels of thrips suppression were observed, equal to those achieved by A. swirskii or A. limonicus treatments in the 2016 trial, and superior to those by N. cucumeris in both trial years. These findings show that T. montdorensis is a good thrips predator, and provides rationale for the development of this species as a new agent for greenhouse pest management in an expanded temperate area of the world.

The function of supplemental foods for improved crop establishment of generalist predators Orius insidiosus and Dicyphus hesperus.

As with many biological control agents, generalist predators rarely survive prolonged periods of prey scarcity. Towards improving crop establishment of two major predators used in North America, Orius insidiosus and Dicyphus hesperus, this study examined the role of supplemental foods in achieving greater predator survival and faster development. In controlled environment trials, developmental time and survival were compared for predators offered diets including Ephestia eggs, Artemia cysts, Typha pollen, or combinations of these. Nymphal developmental time was significantly shorter and survival greater for both predators reared on diets that included Ephestia eggs. Interestingly, D. hesperus could successfully complete nymphal development on Artemia cysts whereas O. insidiosus could not, alluding to fundamental physiological differences between these predators. In greenhouse assays, D. hesperus was more abundant after six weeks when offered diets that included Ephestia eggs either alone or in combination with pollen or Artemia cysts relative to other diets. In contrast, only diets of Ephestia eggs, Typha pollen or their combination could significantly increase O. insidiosus crop abundance relative to the unfed control. Together, this work highlights important differences in the relative values of supplemental foods for generalist predators used in crop protection. It is also meaningful in guiding biocontrol practitioners globally in the rapidly growing sector of greenhouse vegetable production.

Histone lysine demethylase (KDM) subfamily 4: structures, functions and therapeutic potential.

KDM4 histone demethylases catalyze the removal of methyl marks from histone lysine residues to epigenetically regulate chromatin structure and gene expression. KDM4 expression is tightly regulated to insure proper function in diverse biological processes, such as cellular differentiation. Mounting evidence has shown that disrupting KDM4 expression is implicated in the establishment and progression of multiple diseases including cancer. In particular, genomic regions encoding the KDM4A, B and C genes are often amplified, disrupting normal cellular proliferation. Furthermore, KDM4 demethylases are promising druggable targets. In this review, we highlight the latest advances in characterizing the structures and regulatory mechanisms of KDM4 proteins, as well as our current understanding of their alterations and roles in tumorigenesis. We also review the reported KDM4 inhibitors and discuss their potential as therapeutic agents.

A comparative transcriptomic analysis reveals conserved features of stem cell pluripotency in planarians and mammals.

Many long-lived species of animals require the function of adult stem cells throughout their lives. However, the transcriptomes of stem cells in invertebrates and vertebrates have not been compared, and consequently, ancestral regulatory circuits that control stem cell populations remain poorly defined. In this study, we have used data from high-throughput RNA sequencing to compare the transcriptomes of pluripotent adult stem cells from planarians with the transcriptomes of human and mouse pluripotent embryonic stem cells. From a stringently defined set of 4,432 orthologs shared between planarians, mice and humans, we identified 123 conserved genes that are ≥5-fold differentially expressed in stem cells from all three species. Guided by this gene set, we used RNAi screening in adult planarians to discover novel stem cell regulators, which we found to affect the stem cell-associated functions of tissue homeostasis, regeneration, and stem cell maintenance. Examples of genes that disrupted these processes included the orthologs of TBL3, PSD12, TTC27, and RACK1. From these analyses, we concluded that by comparing stem cell transcriptomes from diverse species, it is possible to uncover conserved factors that function in stem cell biology. These results provide insights into which genes comprised the ancestral circuitry underlying the control of stem cell self-renewal and pluripotency.

Expression of multidrug resistance proteins is localized principally to the Malpighian tubules in larvae of the cabbage looper moth, Trichoplusia ni.

The multidrug resistance proteins (MRPs) serve a number of important roles in development, physiological homeostasis and metabolic resistance. In insects, they may also contribute to resistance against xenobiotics including insecticides and plant secondary metabolites. To investigate their contribution to xenobiotic resistance, we have examined the tissue distribution of gene and protein expression of the multidrug resistance proteins TrnMRP1 and TrnMRP4 of the lepidopteran insect, Trichoplusia ni. Using quantitative PCR and immunohistochemistry, we have identified high expression levels of both transporters in the Malpighian tubules relative to levels in other major tissues of the body, where they probably contribute to excretion of metabolic wastes or ingested xenobiotics. We have specifically located TrnMRP protein expression in a subpopulation of Malpighian tubule secondary cells. Expression of TrnMRP1 was also detected both at a high level in specific cortical neurons of larval ganglia and at a lower level throughout the cortex, where it may act in signaling or protective functions, respectively. In contrast, expression of TrnMRP4 was low to absent in larval ganglia, with the exception of single cells in the central connective. We discuss the potential implications of this TrnMRP activity on insect development and metabolic resistance.