ABSTRACT
A truncated form of the structural protein VP2 (truncVP2) of infectious pancreatic necrosis (IPN) virus encompassing amino acids 147-307 was expressed in bacterial, yeast, piscine and mammalian cells. All four recombinant antigens were recognised by a VP2-specific monoclonal antibody by ELISA and immunoblot analysis. However, the minimum amount of r-truncVP2 needed for detection by these methods varies depending on the cell type used for expression. Furthermore, all four recombinant preparations, when used to immunise Atlantic salmon, were capable of inducing antibodies reactive with whole IPNV in ELISA.
Subject(s)
Antigens, Viral/immunology , Birnaviridae Infections/immunology , Capsid/immunology , Infectious pancreatic necrosis virus/immunology , Peptide Fragments/immunology , Salmon/immunology , Animals , Antibodies, Viral/blood , Antigens, Viral/biosynthesis , Antigens, Viral/genetics , Base Sequence , Birnaviridae Infections/prevention & control , Blotting, Western/veterinary , CHO Cells , Capsid/genetics , Capsid Proteins , Cricetinae , Enzyme-Linked Immunosorbent Assay/veterinary , Epitopes/immunology , Escherichia coli/genetics , Immunization/veterinary , Infectious pancreatic necrosis virus/genetics , Molecular Sequence Data , Peptide Fragments/biosynthesis , Peptide Fragments/genetics , Pichia/genetics , Recombinant Proteins/chemistry , Recombinant Proteins/genetics , Recombinant Proteins/immunology , Viral Vaccines/immunology , Viral Vaccines/standardsABSTRACT
The Wnt family of developmental genes has previously been shown to be involved in proliferation, differentiation, and cell to cell signaling during embryogenesis. In addition, several Wnt genes have been shown to be expressed during carcinogenesis. We have investigated these genes during the wound-healing process. Wnt-4 gene expression is found in mouse wounds from 2 hours to 30 hours postwounding. The expression of Wnt-4 is also stimulated by direct trauma to murine fibroblasts in culture, and the expression is greatly enhanced by the addition of a short plasmin digest of fibrin. Therefore the regulation of Wnt-4, appears to be complex, with expression being stimulated both by direct trauma and by the influence of clotting and fibrinolysis products. We propose that the expression of Wnt-4 in the early wound, in response to the provisional fibrin matrix, regulates cell movement and proliferation in the creation of new tissue by mechanisms related to those of embryogenesis.
