ABSTRACT
Water-in-oil-type adjuvants prepared with natural or synthetic vitamin E as the oil phase, Arlacel A or Montanide 103 as emulsifier and 1.0% aqueous solution of bovine serum albumin (BSA) as antigen were tested in mice. An emulsion of 0.42 ml vitamin E, 0.42 ml special mineral oil, 0.15 ml Arlacel A and 1.0 ml BSA gave maximal humoral response, but 0.85 ml special mineral oil, 0.15 ml Arlacel A and 1.0 ml BSA (standard Freund's incomplete adjuvant formulation) gave the greatest delayed-type hypersensitivity response.
Subject(s)
Adjuvants, Immunologic/chemistry , Vitamin E/analogs & derivatives , Vitamin E/immunology , alpha-Tocopherol/analogs & derivatives , Animals , Antibody Formation , Dimethyl Sulfoxide , Emulsions , Female , Freund's Adjuvant , Hypersensitivity, Delayed , Male , Mice , Mineral Oil , Serum Albumin, Bovine/immunology , Tocopherols , Vitamin E/analysisABSTRACT
The effect of beta carotene on disease protection and humoral immunity in chickens was investigated in comparison with the effect of other lipid-soluble antioxidant vitamins, vitamin E and A, which are both proven immunoenhancers and contributors to disease protection. Beta carotene alone was not as effective as either vitamin in protecting chickens from Escherichia coli infection, nor did it significantly enhance humoral immunity. In combination with vitamin E, however, beta carotene significantly increased disease protection and reduced hepatomegaly caused by E. coli infection.
