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1.
Forensic Sci Int ; 236: 151-6, 2014 Mar.
Article in English | MEDLINE | ID: mdl-24529787

ABSTRACT

For several years, hair analyses have become a powerful tool to investigate past exposure towards xenobiotics. In the case of illicit drugs and more precisely of cannabis exposure, four compounds are usually investigated: Δ(9)-tetrahydrocannabinol (THC), the main active compound of cannabis, one of its metabolites [11-nor-Δ(9)-tetrahydrocannabinol-9-carboxylic acid (THC-COOH)] and two cannabinoids (cannabinol and cannabidiol). Up until now, the hair determination of the carboxylic metabolite of THC, which has been described as the only marker allowing distinguishing consumption and passive exposure, has been performed using a gas chromatography-tandem mass spectrometry method. The aim of this study was to develop a liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for the simultaneous quantitative determination of the four markers. The sample preparation was based on an alkaline hydrolysis of hair samples followed by a liquid-liquid extraction of compounds in acidic conditions using a hexane/ethyl acetate mixture. The method was validated and the results were satisfactory: intra- and inter-assay accuracies below 9% and relative standard deviation below 15% for the four compounds. Moreover, the limit of quantification for THC-COOH, the most challenging compound, was validated at 0.2 pg/mg. This concentration is in accordance with the recommendations made by a scientific society which specializes in hair testing. It makes it possible to distinguish the kind of exposure to cannabis.


Subject(s)
Cannabidiol/analysis , Dronabinol/analogs & derivatives , Dronabinol/analysis , Hair/chemistry , Hallucinogens/analysis , Chromatography, Liquid , Forensic Toxicology , Humans , Tandem Mass Spectrometry
2.
Forensic Sci Int ; 234: 132-8, 2014 Jan.
Article in English | MEDLINE | ID: mdl-24378313

ABSTRACT

The two major challenges in hair analysis are the limited amount of samples usually available and the low targeted concentrations. To overcome these limitations, a liquid chromatography-electrospray-tandem mass spectrometry method (LC-ESI-MS/MS) allowing the simultaneous analysis of 17 amphetamines (amphetamine, BDB, m-CPP, dexfenfluramine, DOB, DOM, ephedrine, MBDB, MDA, MDEA, MDMA, methamphetamine, methylphenidate, 4-MTA, norephedrine, norfenfluramine and PMA), 5 opiates (morphine, codeine, heroin, ethylmorphine, and 6AM), cocaine and 5 metabolites [ecgonine methyl ester (EME), benzoylecgonine (BZE), anhydroecgonine methyl ester (AME), cocaethylene, and norcocaine] has been developed. The validation procedure included linearity, intra-day and inter-day variability and accuracy for 5 days (5 replicates at 3 concentration levels). Proficiency studies were used to check the accuracy of the method. As a result, all amphetamines, opiates and cocaine derivatives were satisfactory identified by 2 MRM transitions in 15 min. Calibration curves were performed by a quadratic 1/X weighted regression. The calibration model fits from 0.05 to 10 ng/mg. The limits of detection (LODs) range between 0.005 and 0.030 ng/mg. Precision has been checked by intra-day and inter-day RSD, and associated relative bias, which were lower than 25% for the limits of quantifications (LOQs) and lower than 20% for the other levels tested. This method was routinely applied to hair samples: two positive results of adult drug addicts are presented.


Subject(s)
Amphetamines/analysis , Analgesics, Opioid/analysis , Cocaine/analysis , Hair/chemistry , Narcotics/analysis , Chromatography, Liquid , Cocaine/analogs & derivatives , Forensic Toxicology/methods , Humans , Limit of Detection , Male , Spectrometry, Mass, Electrospray Ionization , Substance Abuse Detection/methods , Tandem Mass Spectrometry
3.
Forensic Sci Int ; 228(1-3): e20-4, 2013 May 10.
Article in English | MEDLINE | ID: mdl-23522525

ABSTRACT

We reported one non fatal case (42 month old boy) of intoxication with diazinon following accidental ingestion. Diazinon and three of its metabolites (2 common metabolites with other organophosphate pesticides: diethylphosphate and diethylthiophosphate; one specific metabolite: 2-isopropyl-4-methyl-6-hydroxypyrimidine) were determined in serum and in urine, respectively, using three liquid chromatography-tandem mass spectrometry methods. Diazinon was detected in serum while its metabolites were detected in urine. The concentrations of diazinon and its common metabolites were compared to concentrations previously described in literature in the same intoxication context and were discussed. The concentration of the specific metabolite was compared to concentrations highlighted in occupational exposure, because to the best of our knowledge, we reported here the first urinary concentration of this metabolite in an acute intoxication context.


Subject(s)
Diazinon/urine , Insecticides/urine , Pyrimidines/urine , Accidents , Child, Preschool , Chromatography, Liquid , Diazinon/administration & dosage , Diazinon/blood , Forensic Toxicology , Humans , Insecticides/administration & dosage , Insecticides/blood , Limit of Detection , Male , Tandem Mass Spectrometry
4.
Forensic Sci Int ; 226(1-3): e20-5, 2013 Mar 10.
Article in English | MEDLINE | ID: mdl-23291146

ABSTRACT

Acute intoxications after ingesting glyphosate are observed in suicidal or accidental cases. Despite low potential toxicity of this herbicide, a number of fatalities and severe outcomes are reported. Indeed, some authors have described the clinical features associated with blood and urine concentrations following intoxication. The purpose of this study is to describe the clinical feature and determinate the utility of the glyphosate concentration in blood and urine and the dose taken for predicting clinical outcomes. In 13 glyphosate poisoning cases treated in our laboratory within 7 years period from 2002 to 2009, we registered clinical observations and collected blood and urine samples to HPLC-MS-MS analysis. We classified our patients by the intoxication severity using simple clinical criteria. We obtained clinical observations from 10 patients and the others three patients were treated in forensic cases. Among the 10 patients, one was asymptomatic, 5 had mild to moderate poisoning and 2 had severe poisoning. There were 6 deaths whose 3 were forensic cases. The most common symptoms were oropharyngeal ulceration (5/10), nausea and vomiting (3/10). The main altered biological parameters were high lactate (3/10) and acidosis (7/10). We also noted respiratory distress (3/10), cardiac arrhythmia (4/10), hyperkaleamia, impaired renal function (2/10), hepatic toxicity (1/10) and altered consciousness (3/10). In fatalities, the common symptoms were cardiovascular shock, cardiorespiratory arrest, haemodynamic disturbance, intravascular disseminated coagulation and multiple organ failure. Blood glyphosate concentrations had a mean value of 61 mg/L (range 0.6-150 mg/L) and 4146 mg/L (range 690-7480 mg/L) respectively in mild-moderate intoxication and fatal cases. In the severe intoxication case for which blood has been sampled, the blood glyphosate concentration was found at 838 mg/L. Death was most of the time associated with larger taken dose (500 mL in one patient) and high blood glyphosate concentrations. To predict clinical outcomes and to guide treatment support in patients who ingested glyphosate, blood concentrations of this compound and the taken dose have been useful.


Subject(s)
Glycine/analogs & derivatives , Herbicides/blood , Herbicides/urine , Organophosphonates/blood , Organophosphonates/urine , Accidents , Acidosis/chemically induced , Adult , Aged , Arrhythmias, Cardiac/chemically induced , Chemical and Drug Induced Liver Injury/etiology , Chromatography, High Pressure Liquid , Consciousness Disorders/chemically induced , Deglutition Disorders/chemically induced , Disseminated Intravascular Coagulation/chemically induced , Female , Forensic Toxicology , Glycine/adverse effects , Glycine/blood , Glycine/poisoning , Glycine/urine , Heart Arrest/chemically induced , Hemodynamics , Herbicides/adverse effects , Herbicides/poisoning , Humans , Hyperkalemia/chemically induced , Isoxazoles , Lactic Acid/blood , Male , Mass Spectrometry , Middle Aged , Multiple Organ Failure/chemically induced , Nausea/chemically induced , Organophosphonates/adverse effects , Oropharynx/pathology , Pharyngeal Diseases/chemically induced , Pharyngitis/chemically induced , Renal Insufficiency/chemically induced , Respiratory Distress Syndrome/chemically induced , Shock/chemically induced , Suicide , Tetrazoles , Ulcer/chemically induced , Vomiting/chemically induced , Glyphosate
5.
Toxicol Lett ; 210(2): 248-53, 2012 Apr 25.
Article in English | MEDLINE | ID: mdl-21893175

ABSTRACT

A sensitive and reliable method was developed and validated for the determination of five synthetic pyrethroid metabolites namely cis-Cl(2)CA, trans-Cl(2)CA, Br(2)CA, 3-PBA and 4-FPBA in human urine by liquid chromatography-tandem mass spectrometry. (2)D(6)-labelled trans-Cl(2)CA and (13)C(6)-labelled 3-PBA were used as internal standards. This method was based on a liquid-liquid extraction procedure in acidic conditions using hexane solvent with a basic purification, a chromatographic separation using a specific C18 column and mass spectrometric detection in the negative polarity. Suitable limits of detection (0.015µg/L for the five compounds) and quantification (from 0.020 to 0.030µg/L) were obtained for rendering the method usable for the biomonitoring of pyrethroids in the general population. The efficiency of the method was tested in 39 urine samples from French people without any known exposure to pyrethroids. At least three of the five metabolites were detected in each sample. The results of this study were compared to those obtained in previous ones and discussed.


Subject(s)
Chromatography, Liquid/methods , Insecticides/chemistry , Insecticides/urine , Pyrethrins/chemistry , Pyrethrins/urine , Tandem Mass Spectrometry/methods , Adult , Environmental Exposure , Environmental Monitoring/methods , Environmental Pollutants , Female , Humans , Male , Middle Aged , Molecular Structure , Reproducibility of Results , Sensitivity and Specificity , Young Adult
6.
Forensic Sci Int ; 212(1-3): e10-4, 2011 Oct 10.
Article in English | MEDLINE | ID: mdl-21640530

ABSTRACT

We present two non fatal cases of intoxication with carbofuran (CBF) documented by hair analysis. Carbofuran and 3-hydroxycarbofuran (OHCBF, its main metabolite) hair concentrations were determined using a liquid chromatography-tandem mass spectrometry method. The obtained results were surprising if we consider several hair analyses previously published and based on a theory of the presence of xenobiotic in the only segment that comprised its intake. Among the two intoxication cases, we noticed the presence of CBF and OHCBF in hair segments corresponding to 45 days before, and more than 100 days after, the day of intoxication. Additionally, repeated hair samplings and subsequent analysis revealed a decrease of the carbofuran's concentration during the hair life.


Subject(s)
Carbofuran/analogs & derivatives , Carbofuran/poisoning , Forensic Toxicology/methods , Hair/chemistry , Carbofuran/analysis , Follow-Up Studies , France , Gas Chromatography-Mass Spectrometry , Humans , Insecticides/analysis , Insecticides/poisoning , Male , Middle Aged , Risk Assessment , Suicide, Attempted , Time Factors
8.
Forensic Sci Int ; 176(1): 72-5, 2008 Mar 21.
Article in English | MEDLINE | ID: mdl-17983717

ABSTRACT

We reported two non-fatal cases of intoxication with pesticides namely alachlor and carbofuran. Hair stand samples were collected from two men approximately 1 year after alachlor intoxication for case 1, and 14 days after the last exposure for case 2. Hair analysis was performed using a liquid chromatography-tandem mass spectrometry method. In case 1, alachlor was detected in the 5 analysed hair segments (concentrations between 12 and 136 pg/mg) and its metabolites were not detected. In case 2, carbofuran and its main metabolite (3-hydroxycarbofuran) were detected in the hair strand (global analysis) at the concentrations of 207 and 164 pg/mg, respectively. However, additional data are required in order to interpret such results.


Subject(s)
Acetamides/analysis , Carbofuran/analysis , Hair/chemistry , Pesticides/analysis , Adult , Carbofuran/analogs & derivatives , Forensic Toxicology , Gas Chromatography-Mass Spectrometry , Humans , Male , Middle Aged , Occupational Exposure
9.
Ann Pharm Fr ; 64(5): 312-27, 2006 Sep.
Article in French | MEDLINE | ID: mdl-17095951

ABSTRACT

ICP-MS is an instrumental method of multi-elementary qualitative and quantitative analysis. It associates with a mass spectrometer (MS) an ion source composed of a plasma torch fed with inductive coupling with a high frequency electromagnetic generator (ICP), similar to that used as a light source in highly-successful Atomics Emission Spectrometry (AES). ICP-MS can be applied to simultaneous analysis of numerous metallic and metalloid elements (80 or so). Its sensitiveness is all in all far better than that available with previous spectrometric techniques, which nevertheless remain more advantageous for processing certain low atomic mass elements. Thanks to its broad dynamic range, ICP-MS allows quantification of an array of elementary concentrations within a single sample. ICP-MS offers particularly interesting perspectives in geochemistry and metal processing, as well as in biochemistry and food or toxicology and environmental analysis. Implementation is rapid and the technique is suitable for series or continuous analyses, or for analysis of any evolving medium such as effluents from gas or liquid chromatography or from capillary electrophoresis, making it a valuable tool for speciation analyses. Finally it enables non-radioactive isotopic labeling, essential for nutritional studies of trace elements, and sufficiently accurate isotopic dilutions, even with more accessible machines.


Subject(s)
Mass Spectrometry/instrumentation , Animals , Biology/instrumentation , Humans , Mass Spectrometry/trends , Terminology as Topic
10.
Acta Clin Belg ; 61 Suppl 1: 71-6, 2006.
Article in French | MEDLINE | ID: mdl-16700158

ABSTRACT

Currently, the determination of pesticides in human fluids is complex. Our scientific knowledge on pesticide intoxication is fragmentary. This leads to numerous difficulties regarding the diagnosis, the analysis request, the choice of the analytical technique, the interpretation of the results. Consequently the waiting time to provide a result is not compatible with the initiation of a treatment in the intoxicated patients. Very few information about Human intoxication with pesticides is available from the literature. In this context, we tried to acquire our own experience. We chose to deal with all pesticides intoxication suspicions received, in collaboration with clinicians. We are presenting the main problems we encounter.


Subject(s)
Pesticides/analysis , Pesticides/poisoning , Hair/chemistry , Humans , Pesticides/blood , Pesticides/urine
11.
J Chromatogr B Analyt Technol Biomed Life Sci ; 831(1-2): 223-9, 2006 Feb 02.
Article in English | MEDLINE | ID: mdl-16412711

ABSTRACT

Dialkylphosphates (DAP) are urinary markers of the exposure to organophosphates pesticides. The aim of this study was to develop a liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for the simultaneous quantitative determination of the following DAP: dimethylphosphate (DMP), dimethythiophosphate (DMTP), dimethyldithiophosphate (DMDTP), diethylphosphate (DEP), diethylthiophosphate (DETP) and diethyldithiophosphate (DEDTP). Dibutylphosphate (DBP) was used as internal standard. This method was based on a liquid-liquid extraction procedure, a chromatographic separation using an Inertsil ODS3 C18 column and mass spectrometric detection in the negative ion, multiple reaction monitoring (MRM) mode, following two ion transitions per compound. It yielded a limit of quantification of 2 microg/L for the six compounds and intra-assay coefficients of variation (CV%) lower than 20%. This method was applied to the analysis of urines samples from a small cohort of non-exposed volunteers. At least one of the six DAP was detected in each sample. This result confirmed the feasibility of a LC-MS/MS procedure for monitoring the general population exposure to some frequently employed organophosphate pesticides.


Subject(s)
Chromatography, High Pressure Liquid/methods , Mass Spectrometry/methods , Organophosphates/urine , Adult , Environmental Exposure/analysis , Female , Humans , Male , Middle Aged , Pesticide Residues/urine
12.
Ther Drug Monit ; 28(1): 123-30, 2006 Feb.
Article in English | MEDLINE | ID: mdl-16418706

ABSTRACT

Antidepressants belong to a variety of chemical and pharmacologic classes. Most require therapeutic drug monitoring, at least in certain circumstances, such as unexplained inefficacy or suspected toxicity. Several types of chromatographic methods have generally been used. This paper presents a fully automated, sensitive, and specific method for the therapeutic drug monitoring of 13 antidepressants of all classes (amoxapine, amitriptyline, citalopram, clomipramine, dothiepin, doxepin, fluoxetine, imipramine, maprotiline, mianserin, paroxetine, sertraline, trimipramine) and some of their respective active metabolites (nortriptyline, monodesmethylcitalopram, desmethylclomipramine, desipramine, norfluoxetine, desmethylmianserin, N-desmethylsertraline), based on the innovative turbulent-flow liquid chromatography (TFC) technology, coupled to tandem-mass spectrometry (MS/MS). The antidepressants were divided in two groups depending on their chromatographic properties, so that two injections would be necessary to screen all compounds (which is infrequent for therapeutic drug monitoring). Calibration curves ranged from 10 to 500 ng/mL. No significant memory effect was observed after the injection of a blank serum sample spiked at 500 ng/mL. The intra-assay and inter-assay precision CVs ranged from 0.4% to 12% and from 1% to 16%, respectively. The method was further validated by blindly analyzing Heathcontrol-Therapeutic Drugs Scheme samples (Cardiff Bioanalytical Services Ltd.) containing several antidepressants.


Subject(s)
Antidepressive Agents/blood , Chromatography, Liquid/methods , Mass Spectrometry/methods , Chromatography, Liquid/instrumentation , Humans , Mass Spectrometry/instrumentation
13.
Anal Bioanal Chem ; 383(2): 167-73, 2005 Sep.
Article in English | MEDLINE | ID: mdl-16132130

ABSTRACT

Quantification of chromium in whole blood has been performed by ICP-quadrupole MS. The spectrometer was equipped with a dynamic reaction cell (DRC) with ammonia as reaction gas. The rejection parameter q (RPq) of the DRC and the flow rate of ammonia (NH3) were optimized and set at 0.7 and 0.6 mL min(-1), respectively. Blood was diluted 1:51 (v/v) with an aqueous solution containing 0.1 mg L(-1) NH4OH, 0.1 g L(-1) EDTA, 5 mg L(-1) n-butanol, and 0.1 per thousand Triton X100. Non-spectral matrix effects observed when using the DRC were confirmed by use of vanadium. External calibration with blank and standard solutions prepared in purified water led to biased results for quality control samples. Standard addition calibration was therefore used and its validity verified. By comparing the slopes and calculating residues, it was proved that the plot obtained with standard additions and the plot obtained from blood samples of different concentrations were aligned down to 0.05 microg L(-1) after dilution.


Subject(s)
Chromium/blood , Mass Spectrometry/methods , 1-Butanol/chemistry , Ammonia/chemistry , Ammonium Hydroxide , Calibration , Edetic Acid/chemistry , Hydroxides/chemistry , Octoxynol/chemistry , Quality Control , Reproducibility of Results , Vanadium/analysis
14.
Ann Biol Clin (Paris) ; 62(5): 529-38, 2004.
Article in French | MEDLINE | ID: mdl-15355803

ABSTRACT

Rape drugs or compounds used for chemical submission are current hot topics of numerous media based on a few well-documented identified cases. In the aim of considering the compounds potentially involved and subsequently the samples to collect and the toxicological analyses to perform, and according to the aggressor's viewpoint (victim submission and impunity of himself or herself), the characteristics of such compounds were drawn following the drug pharmacological properties. The compounds or therapeutic classes potentially used are numerous and diverse because the expected effects can be obtained by many neuropharmacological mechanisms or combinations of mechanisms. However, a few drugs (i.e. several benzodiazepines, and gamma-hydroxybutyrate) seem to be the ideal candidates owing to advantageous pharmacological properties (low blood concentrations, short elimination half-life) and practical ones (availability, galenic forms). It appears that the quality and precocity of biological specimen collection, the use of specific and sensitive analytical techniques, and the collaboration between the clinician and the toxicologist, are the essential keys for successful toxicological investigations when a case of chemical submission is suspected.


Subject(s)
Illicit Drugs/analysis , Illicit Drugs/pharmacology , Rape , Female , Hallucinogens/analysis , Hallucinogens/pharmacology , Humans , Hypnotics and Sedatives/analysis , Hypnotics and Sedatives/pharmacology , Male
15.
J Anal Toxicol ; 27(1): 7-14, 2003.
Article in English | MEDLINE | ID: mdl-12587676

ABSTRACT

A complete general unknown screening procedure was developed using liquid chromatography-mass spectrometry (LC-MS), a coupling that can increase the range of compounds amenable to MS. Sample preparation was by solid-phase extraction on a mixed-mode support in parallel with serum deproteination in order to recover the most hydrophilic compounds. Chromatography employed a reversed-phase narrow-bore column (150 x 1-mm i.d.) and a 50-min gradient elution at low flow-rate (50 microL/min), compatible with the electrospray source used without splitting nor heating. The single quadrupole LC-MS instrument used was operated in the 100 to 1100 mu mass range in both the positive and negative modes, with two different, alternated collision-induced dissociation voltages in the source, in order to obtain the molecular or pseudo-molecular ions as well as fragments for the compounds analyzed. The addition of spectra obtained at low and high fragmentation voltages gave reconstructed spectra for each polarity, representing library entries. Finally, a program was created in order to detect the peaks of interest in the chromatographic noise using a very efficient signal processing algorithm, compute their relative retention time with respect to the internal standard (glafenine), draw their reconstructed spectra, search them in the libraries, and edit a report.


Subject(s)
Chromatography, High Pressure Liquid/methods , Mass Screening/methods , Pharmaceutical Preparations/blood , Spectrometry, Mass, Electrospray Ionization/methods , Xenobiotics/blood , Humans , Pharmaceutical Preparations/analysis , Xenobiotics/analysis
17.
Acta Clin Belg ; 57 Suppl 1: 41-6, 2002.
Article in French | MEDLINE | ID: mdl-11974443

ABSTRACT

"Rave parties", all-night dance parties based on "techno" music, represent an increasing phenomenon in France. "Rave drugs" refers to a wide variety of drugs used by the young participants owing to their hallucinogenic or stimulant effects. Uncertainties about the sources of these substances, the possible contaminants and the multiplicity of the associations make it difficult to evaluate the toxic consequences that might be expected in this particular context. This report presents toxicological cases documented by analytical findings in which two pharmacological agents abused in "rave parties" in South-West of France were found. The day following a party, a 17 year-old girl showed a confused state with drowsiness and hallucinations. She confessed having consumed a white powder sold as "ecstasy", that sample analysis identified as pure ketamine. Ketamine is an anaesthetic agent primarily used in veterinary medicine and paediatrics. This drug seems to be abused, mainly by the intranasal route, owing to its hallucinogen effects. Its used in "rave-party" appears to be marked by unsuspected consumption. All long another party, a large quantity of orange tablets were sold and abused by several participants. Analysis performed on some fragments of these tablets revealed the presence of prolintane and ascorbic acid. Prolintane, an amphetamine-related substance, is a central nervous system stimulant. This compound is "freely" available in Spain in combination with several vitamins, under the form of tablets with orange coating named "Katovit" and sold at low price: 1.93 [symbol: see text]/20 tablets (200 mg of prolintane).


Subject(s)
Anesthetics, Dissociative/adverse effects , Central Nervous System Stimulants/adverse effects , Ketamine/adverse effects , Pyrrolidines/adverse effects , Recreation , Substance-Related Disorders , Adolescent , Adolescent Behavior , Anesthetics, Dissociative/economics , Central Nervous System Stimulants/economics , Costs and Cost Analysis , Drug Overdose , Female , France , Humans , Ketamine/economics , Pyrrolidines/economics
18.
Acta Clin Belg ; 57 Suppl 1: 41-6, 2002.
Article in English | MEDLINE | ID: mdl-24862524

ABSTRACT

"Rave parties", all-night dance parties based on "techno" music, represent an increasing phenomenon in France. "Rave drugs" refers to a wide variety of drugs used by the young participants owing to their hallucinogenic or stimulant effects. Uncertainties about the sources of these substances, the possible contaminants and the multiplicity of the associations make it difficult to evaluate the toxic consequences that might be expected in this particular context. This report presents toxicological cases documented by analytical findings in which two pharmacological agents abused in "rave parties" in South-West of France were found. The day following a party, a 17 year-old girl showed a confused state with drowsiness and hallucinations. She confessed having consumed a white powder sold as "ecstasy", that sample analysis identified as pure ketamine. Ketamine is an anaesthetic agent primarily used in veterinary medicine and paediatrics. This drug seems to be abused, mainly by the intranasal route, owing to its hallucinogen effects. Its used in "rave-party" appears to be marked by unsuspected consumption. All long another party, a large quantity of orange tablets were sold and abused by several participants. Analysis performed on some fragments of these tablets revealed the presence of prolintane and ascorbic acid. Prolintane, an amphetamine-related substance, is a central nervous system stimulant. This compound is "freely" available in Spain in combination with several vitamins, under the form of tablets with orange coating named "Katovit" and sold at low price: 1.93 €/20 tablets (200 mg of prolintane).

19.
J Forensic Sci ; 46(6): 1507-9, 2001 Nov.
Article in English | MEDLINE | ID: mdl-11714169

ABSTRACT

An alcoholic man, treated with chloral hydrate (CH) syrup to which he was dependent, was discovered comatose and in respiratory arrest. Death occurred on the ninth day of hospitalization following cerebral oedema. A woman, alcohol addicted, depressed, and epileptic was admitted in the Intensive Care Unit with heart and respiratory failure following CH absorption. She died three days later after a deep coma. In these two cases, CH intoxication was confirmed by toxicological analysis: CH and its major metabolite, trichloroethanol (TCE), were identified and determined in serum and urine using headspace-capillary gas chromatography-mass spectrometry. The concentrations measured were compared with those found in previously published fatalities. The analytical method used can be proposed for both clinical and forensic cases.


Subject(s)
Chloral Hydrate/poisoning , Ethylene Chlorohydrin/analogs & derivatives , Hypnotics and Sedatives/poisoning , Adult , Alcoholism/drug therapy , Chloral Hydrate/blood , Chloral Hydrate/urine , Drug Overdose , Ethylene Chlorohydrin/blood , Ethylene Chlorohydrin/poisoning , Ethylene Chlorohydrin/urine , Fatal Outcome , Female , Gas Chromatography-Mass Spectrometry , Humans , Hypnotics and Sedatives/blood , Hypnotics and Sedatives/urine , Male , Substance-Related Disorders/etiology
20.
J Chromatogr B Biomed Sci Appl ; 759(1): 109-16, 2001 Aug 05.
Article in English | MEDLINE | ID: mdl-11499614

ABSTRACT

This paper describes a rapid, specific and sensitive method for the determination of 29 organophosphorus pesticides in blood and serum, involving a rapid solid-phase extraction procedure using Oasis HLB cartridges and gas chromatography coupled to mass-selective detection. The ionization was performed by electron Impact and acquisition in the single ion monitoring mode followed three specific ions per analyte. Extraction recoveries were satisfactory and ranged between 40 and 108% in blood and serum. Limits of detection ranged from 5 to 25 ng/ml and limits of quantitation (LOQs) ranged from 10 to 50 ng/ml, in blood and serum. An excellent linearity was observed from these LOQs up to 1000 ng/ml. Intra- and inter-assay precision and accuracy were satisfactory for most of the pesticides analyzed.


Subject(s)
Gas Chromatography-Mass Spectrometry/methods , Insecticides/blood , Organophosphorus Compounds , Pesticide Residues/blood , Hydrogen-Ion Concentration , Reproducibility of Results , Sensitivity and Specificity
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