Subject(s)
Communication , Hematology , Internal Medicine , Interprofessional Relations , Specialization , HumansABSTRACT
Chinese hamster embryo cells were transformed with temperature-sensitive mutant of the Schmidt-Ruppin strain of Rous sarcoma virus-subgroup A (ts NY68S Ra-A). The developed cell line elicited the morphological transformation at a permissive temperature of 35 degrees C, whereas after a shift to a non-permissive temperature of 40 degrees C the cells reverted to a normal phenotype. Tests for the presence of avian sarcoma group-specific antigen showed that the antigen was not expressed both at permissive and non-permissive temperature. However, the transient expression of gs antigen could be achieved by treatment with 5-IUdR only at the permissive temperature. There was a difference in the rate of 14C-2-deoxy-D-glucose uptake between cells cultured at the permissive and non-permissive temperature.
Subject(s)
Avian Sarcoma Viruses , Cytopathogenic Effect, Viral , Animals , Antigens, Viral , Cell Line , Chick Embryo , Cricetinae , Embryo, Mammalian , Glucose/metabolism , TemperatureABSTRACT
The content of avian gs antigen in RSV-transformed rat cells LW13-RsK1 and LW13-RsK4 showed a transient increase after treatment with 5-iododeoxyuridine. Higher levels of gs antigen were found when treated cells were cultivated in the presence of dexamethasone. In no case was the production of RSV found in treated RSV-transformed rat cell lines when, in addition, XC cells were tested.
