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1.
Brain Behav Immun ; 41: 251-60, 2014 Oct.
Article in English | MEDLINE | ID: mdl-24793756

ABSTRACT

BACKGROUND: Recent lines of research have boosted awareness of the immunological facets of schizophrenia. However, associations with protein tyrosine phosphatase regulators have never been reported. The aim of our study was to investigate the expression and promoter status methylation of phosphatase SHP-1, a key negative regulator of the inflammatory process, in Peripheral blood mononuclear cells (PBMCs) of Schizophrenic patients. METHODS: We enrolled fifty-four (28 men and 26 women) unmedicated first episode subjects (SC) who met DSM-IV and thirty-eight (22 men and 16 women) healthy controls (HC). The SC psychopathological status was assessed using the Positive and Negative Syndrome Scale. We evaluated SHP-1 expression by Quantitative Real-time PCR (qPCR) and Western blotting (WB) methods and promoter status methylation through PCR bisulfate. IKK/NFkB signaling was detected by WB, and medium and plasma levels of pro-inflammatory cytokines (IL-1ß, IL-2, and TNF-α) by the ELISA method. SHP-1 was silenced by treating cells with specific siRNA. RESULTS: We found a significantly lower level of SHP-1 gene expression in PBMCs from SC vs. HC, consistently with which the promoter region analyzed presented significant hypermethylation. Silencing of SHP-1 expression induced higher activation of IKK/NF-kB signaling and pro-inflammatory cytokine production in ex vivo PBMCs from both SC and HC. Linear regression among patients generated a model in which SHP-1 expression explained 30% of the clinical negative symptom variance (adjusted R(2)=0.30, ANOVA p<0.001). CONCLUSIONS: Our findings are the first to suggest that impairment of SHP-1 expression is involved in the physiopathology of schizophrenia, opening fruitful new avenues for ameliorating treatment at least of negative symptoms.


Subject(s)
Cytokines/biosynthesis , Protein Tyrosine Phosphatase, Non-Receptor Type 6/physiology , Schizophrenia/enzymology , Adult , C-Reactive Protein/analysis , Cytokines/genetics , DNA Methylation , Female , Humans , I-kappa B Kinase/metabolism , Leukocytes, Mononuclear/enzymology , Male , Middle Aged , NF-kappa B/metabolism , Promoter Regions, Genetic , Protein Tyrosine Phosphatase, Non-Receptor Type 6/antagonists & inhibitors , Protein Tyrosine Phosphatase, Non-Receptor Type 6/biosynthesis , Protein Tyrosine Phosphatase, Non-Receptor Type 6/genetics , RNA Interference , RNA, Messenger/biosynthesis , RNA, Small Interfering/pharmacology , Schizophrenia/genetics , Schizophrenia/immunology , Schizophrenia/physiopathology , Schizophrenic Psychology , Severity of Illness Index
2.
Br J Cancer ; 109(10): 2654-64, 2013 Nov 12.
Article in English | MEDLINE | ID: mdl-24136149

ABSTRACT

BACKGROUND: The DNA-repair gene DNA-dependent kinase catalytic subunit (DNA-PKcs) favours or inhibits carcinogenesis, depending on the cancer type. Its role in human hepatocellular carcinoma (HCC) is unknown. METHODS: DNA-dependent protein kinase catalytic subunit, H2A histone family member X (H2AFX) and heat shock transcription factor-1 (HSF1) levels were assessed by immunohistochemistry and/or immunoblotting and qRT-PCR in a collection of human HCC. Rates of proliferation, apoptosis, microvessel density and genomic instability were also determined. Heat shock factor-1 cDNA or DNA-PKcs-specific siRNA were used to explore the role of both genes in HCC. Activator protein 1 (AP-1) binding to DNA-PKcs promoter was evaluated by chromatin immunoprecipitation. Kaplan-Meier curves and multivariate Cox model were used to study the impact on clinical outcome. RESULTS: Total and phosphorylated DNA-PKcs and H2AFX were upregulated in HCC. Activated DNA-PKcs positively correlated with HCC proliferation, genomic instability and microvessel density, and negatively with apoptosis and patient's survival. Proliferation decline and massive apoptosis followed DNA-PKcs silencing in HCC cell lines. Total and phosphorylated HSF1 protein, mRNA and activity were upregulated in HCC. Mechanistically, we demonstrated that HSF1 induces DNA-PKcs upregulation through the activation of the MAPK/JNK/AP-1 axis. CONCLUSION: DNA-dependent protein kinase catalytic subunit transduces HSF1 effects in HCC cells, and might represent a novel target and prognostic factor in human HCC.


Subject(s)
Biomarkers, Tumor/genetics , Carcinogenesis/genetics , Carcinoma, Hepatocellular/pathology , DNA-Activated Protein Kinase/genetics , Liver Neoplasms/pathology , Nuclear Proteins/genetics , Carcinoma, Hepatocellular/genetics , Cell Line, Tumor , Cell Proliferation , Cell Survival/genetics , DNA-Binding Proteins/physiology , Gene Expression Regulation, Enzymologic , Gene Expression Regulation, Neoplastic , Heat Shock Transcription Factors , Hep G2 Cells , Humans , Liver Neoplasms/genetics , Predictive Value of Tests , Prognosis , Transcription Factors/physiology
3.
Gut ; 58(5): 679-87, 2009 May.
Article in English | MEDLINE | ID: mdl-19136513

ABSTRACT

BACKGROUND AND AIMS: Previous studies indicate unrestrained cell cycle progression in liver lesions from hepatocarcinogenesis-susceptible Fisher 344 (F344) rats and a block of G(1)-S transition in corresponding lesions from resistant Brown Norway (BN) rats. Here, the role of the Forkhead box M1B (FOXM1) gene during hepatocarcinogenesis in both rat models and human hepatocellular carcinoma (HCC) was assessed. METHODS AND RESULTS: Levels of FOXM1 and its targets were determined by immunoprecipitation and real-time PCR analyses in rat and human samples. FOXM1 function was investigated by either FOXM1 silencing or overexpression in human HCC cell lines. Activation of FOXM1 and its targets (Aurora Kinose A, Cdc2, cyclin B1, Nek2) occurred earlier and was most pronounced in liver lesions from F344 than BN rats, leading to the highest number of Cdc2-cyclin B1 complexes (implying the highest G(2)-M transition) in F344 rats. In human HCC, the level of FOXM1 progressively increased from surrounding non-tumorous livers to HCC, reaching the highest levels in tumours with poorer prognosis (as defined by patients' length of survival). Furthermore, expression levels of FOXM1 directly correlated with the proliferation index, genomic instability rate and microvessel density, and inversely with apoptosis. FOXM1 upregulation was due to extracellular signal-regulated kinase (ERK) and glioblastoma-associated oncogene 1 (GLI1) combined activity, and its overexpression resulted in increased proliferation and angiogenesis and reduced apoptosis in human HCC cell lines. Conversely, FOXM1 suppression led to decreased ERK activity, reduced proliferation and angiogenesis, and massive apoptosis of human HCC cell lines. CONCLUSIONS: FOXM1 upregulation is associated with the acquisition of a susceptible phenotype in rats and influences human HCC development and prognosis.


Subject(s)
Carcinoma, Hepatocellular/genetics , Extracellular Signal-Regulated MAP Kinases/genetics , Forkhead Transcription Factors/genetics , Liver Neoplasms/genetics , Animals , Carcinoma, Hepatocellular/pathology , Cell Proliferation , Cell Transformation, Neoplastic/genetics , Forkhead Box Protein M1 , Forkhead Transcription Factors/physiology , Gene Expression Regulation, Neoplastic/genetics , Genes, cdc , Genetic Predisposition to Disease/genetics , Liver/pathology , Liver Neoplasms/pathology , Neovascularization, Pathologic/etiology , Rats , Rats, Inbred F344 , Transcriptional Activation , Up-Regulation
4.
Neuropharmacology ; 41(2): 229-37, 2001 Aug.
Article in English | MEDLINE | ID: mdl-11489459

ABSTRACT

Certain antidepressant drugs exert an anxiolytic action in both humans and rodents. The effects of long-term treatment with imipramine or mirtazapine, two antidepressant drugs with different mechanisms of action, on the response of cortical cholinergic neurons to foot-shock stress or to the anxiogenic drug FG 7142 were investigated in freely moving rats. Chronic treatment with imipramine or mirtazapine reduced the increase in cortical acetylcholine output induced by foot-shock stress by approximately 50%. The same treatment also reduced the sensitivity of cortical cholinergic neurons to the stimulatory effect of acute administration of FG 7142. In contrast, the administration of a single dose of either antidepressant 40 min before foot shock or FG 7142 injection failed to increase the threshold of excitability of cortical cholinergic neurons. These results demonstrate that long-term treatment with either imipramine or mirtazapine reduces the sensitivity of cortical cholinergic neurons to stress or to an anxiogenic drug with an efficacy similar to that of acute administration of benzodiazepines. The neurochemical mechanism responsible for regulation of cholinergic neuron sensitivity might contribute to the modulation of cognitive function associated with emotional and affective disorders.


Subject(s)
Antidepressive Agents/administration & dosage , Carbolines/administration & dosage , Cholinergic Fibers/drug effects , GABA Antagonists/pharmacology , Stress, Physiological/metabolism , Acetylcholine/biosynthesis , Animals , Antidepressive Agents/pharmacology , Antidepressive Agents, Tricyclic/pharmacology , Carbolines/pharmacology , Cholinergic Fibers/metabolism , Imipramine/pharmacology , Male , Mianserin/analogs & derivatives , Mianserin/pharmacology , Mirtazapine , Prefrontal Cortex/drug effects , Prefrontal Cortex/metabolism , Rats , Rats, Sprague-Dawley
5.
J Neurochem ; 76(4): 1212-20, 2001 Feb.
Article in English | MEDLINE | ID: mdl-11181840

ABSTRACT

The effects of long-term treatment with imipramine or mirtazapine, two antidepressant drugs with different mechanisms of action, on the response of cortical dopaminergic neurons to foot-shock stress or to the anxiogenic drug FG7142 were evaluated in freely moving rats. As expected, foot shock induced a marked increase (+ 90%) in the extracellular concentration of dopamine in the prefrontal cortex of control rats. Chronic treatment with imipramine or mirtazapine inhibited or prevented, respectively, the effect of foot-shock stress on cortical dopamine output. Whereas acute administration of the anxiogenic drug FG7142 induced a significant increase (+ 60%) in cortical dopamine output in control rats, chronic treatment with imipramine or mirtazapine completely inhibited this effect. In contrast, the administration of a single dose of either antidepressant 40 min before foot shock, had no effect on the response of the cortical dopaminergic innervation to stress. These results show that long-term treatment with imipramine or mirtazapine inhibits the neurochemical changes elicited by stress or an anxiogenic drug with an efficacy similar to that of acute treatment with benzodiazepines. Given that episodes of anxiety or depression are often preceded by stressful events, modulation by antidepressants of the dopaminergic response to stress might be related to the anxiolytic and antidepressant effects of these drugs.


Subject(s)
Antidepressive Agents/administration & dosage , Anxiety/metabolism , Dopamine/metabolism , Mianserin/analogs & derivatives , Prefrontal Cortex/drug effects , Prefrontal Cortex/metabolism , Animals , Anxiety/chemically induced , Carbolines/antagonists & inhibitors , Dose-Response Relationship, Drug , Drug Administration Schedule , Electroshock , GABA Antagonists , Imipramine/administration & dosage , Male , Mianserin/administration & dosage , Microdialysis , Mirtazapine , Neurons/drug effects , Neurons/metabolism , Rats , Rats, Sprague-Dawley , Time Factors
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