Application of the effects of solvent and dissolved oxygen on the determination of benzo[a]pyrene by constant-wavelength synchronous spectrofluorimetry in smoke-flavouring.

This work studies the effects of solvent and of dissolved oxygen in the determination of benzo[a]pyrene (BaP) by constant-wavelength synchronous spectrofluorimetry in smoke-flavour agents, as confirmation and quantification techniques. The wavelength of the most intense peak at the optimum excitation-emission wavelength interval (20 or 110 nm in most of nine solvents) varied by up to 5 nm, and the detection and quantification limits by a factor of up to 30. The best quantification limit was obtained with DMSO (0.09 mug l(-1)). The deoxygenation of the analyte solution decreased detection and quantification limits, by a variable factor (8-1.13 in the case of n-hexane and DMSO, respectively).