Giant cell granuloma of the lung.

In a 50-year-old man without bronchopulmonary symptoms a round mass lesion close to the hilum of the right lung was detected in a routine chest x-ray and confirmed by computed tomography. Histological examination of two biopsy specimens did not result in a definitive diagnosis. Therefore thoracotomy with enucleation of the focus was performed. The histological picture of the lesion is characterized by connective tissue proliferation, multinucleated giant cells, ossification, localised hemorrhage, deposits of hemosiderin and foci of foam cells. The findings are interpreted as a giant cell granuloma of the lung.

The moral lives of children: The thought of Robert Coles.

Robert Coles combines the insights of literature with the resources of medicine and psychiatry in order to understand and bring healing to people. Using the distinction between character and personality, Coles explores the active moral lives of children. To Coles, the moral purpose of the family is to develop character. Coles's contribution is not a new theoretical approach; rather, it is a literary documentary perspective that seeks to awaken and sensitize our moral imaginations.

Socrates and psychotherapy.

This article examines the basic philosophical ideas of Socrates and how these ideas form a basic part of the philosophical foundation of psychotherapy. His influence is analyzed by observing how Kierkegaard and Nietzsche view his contribution. The controversial thesis of I.F. Stone's recent book,The Trial of Socrates, is used to re-examine the man and his philosophy.

[Immunohistochemical labeling of immunoglobulin antigens in semithin sections of epoxy resin-embedded tissue using the gold-silver technic]. / Immunhistochemische Markierung von Immunglobulin-Antigenen an Semidünnschnitten Epoxidharz-eingebetteten Gewebes mit der Gold-Silber-Technik.

The silver nitrate variant of the immunohistochemical gold-silver technique is applied to label L-chains within immunoglobulin containing cells in semithin sections of epoxy resin embedded human tonsils. Different possibilities of tissue fixation and of reactivation of antigens are tested. The best results are obtained by paraformaldehyde-sublimate fixation of tissue and potassium iodide processing of sections. This method allows a good preservation of both tissue and antigens and a sensitive labelling of the immunoglobulin antigens by means of the gold-silver technique.

[Affinity-histochemical labeling methods using colloidal gold. Overview of developments in the past 15 years]. / Affinitätshistochemische Markierungsverfahren mit kolloidalem Gold. Ubersicht über die Entwicklung in 15 Jahren.

A survey is given of the development of affinity-histochemical labelling methods using colloidal gold for electron and light microscopy. Historical aspects and trends are discussed and principle methodologic variations are illustrated. A comprehensive compilation of previously published data provides a concise overview of technical variations and applications of the colloidal gold method.

Encounter with death: The thought of Robert Jay Lifton.

Robert Jay Lifton begins his work in the psychosocial framework that he takes over from Erik Erikson. Lifton's thought is based upon a central paradigm-"death and the continuity of life." Lifton makes important contributions with his five modes of symbolic immortality and with his investigation of the psychological themes in survivors. The origins and limits of Lifton's thought are critically examined.

Myths, metaphors, and mentors.

This article begins with a discussion of myths as visions that transform life. The primary myth is that of death and rebirth. Metaphors are images which illumine our myths. Therapists are thus both scientists and poets. The process of being a mentor occurs in a variety of relationships. Our search is for an understanding of this process which then transcends the positions of master and disciple. Mentors need to have a sufficient degree of self-transcendence to enable the focus to be on the person's own unique vision. Mentors light sparks which ignite our myths and metaphors and thereby expand our understanding of ourselves. Mentors become catalysts for our growth and thereby facilitate the process of transformation.

[Gold-silver technic--a new immunohistochemical method for detecting cell membrane antigens]. / Die Gold-Silber-Technik--ein neues immunhistochemisches Verfahren zum Nachweis von Zellmembranantigenen.

The gold-silver technique is presented as a new method for immunohistochemical labelling of cell membrane antigens. At first, membrane antigens of mouse lymphocytes, human leukaemic cells, and experimental brain tumour cells are labelled by immunoglobulin- or anti-immunoglobulin-gold complexes. Secondarily, gold colloid particles are made visible in the light microscope by means of subsequent precipitation of metallic silver around the colloid particles according to the principle of photographic development. The gold-silver reaction can be used after direct or indirect gold-labelling of cell membrane antigens. The technique is suitable for both vital and prefixed cells. Labelling of membrane antigens of vital and prefixed cells, however, results in a different pattern of the gold-silver grains. The gold-silver method is easy and quickly to perform, sensitive and not expensive.

Marketing pastoral counseling.

Identifies, suggests understandings, and illustrates applications of marketing principles as they relate to pastoral counseling services. Describes four approaches to marketing pastoral counseling--traditional, educational, marketing, and fund raising--and gives illustrations of each. A director of an established pastoral counseling center responds to the article in terms of "high density" and "low density" principles and suggests that strategies which emulate the medical model may need to be re-examined by contemporary pastoral counselors.

A variant of the Wiedemann-Beckwith syndrome.

The following combination of findings were established by histological examination of biopsy or autopsy material in an 11-day-old male baby: congenital mesoblastic nephroma, nodular renal blastema, dysgenetic pancreatic cyst, "cytomegaly" of the adrenal cortex, hyperplasia and hypertrophy of the islets of Langerhans, hypoplasia of the thymus and the lymphatic tissue. Since some of these changes constitute the characteristic histological findings in the Wiedemann-Beckwith syndrome and the remainder can be readily reconciled with it, the assignment of this case to this syndrome is discussed. Although the three cardinal symptoms are lacking, the combination of findings are interpreted as a variant of the wiedemann-Beckwith syndrome.

[Preparation and application of hybrid antibodies (author's transl)]. / Präparation und Anwendungsmöglichkeiten von Hybridantikörpern.

Use of hybrid antibodies with one specificity for IgG and the other for an electron microscopical marker is particularly suited for exact localizing of cell antigens. The preparation of hybrid antibodies of anti-marker and anti-IgG immune sera is described. Different ways of this preparation are discussed, whereby a method of production of hybrid antibodies by use of a combined marker- and IgG-immunoadsorbent is suggested. Application of hybrid antibodies with one specificity for IgG and the other for either ferritin or a plant virus (Erysimum latent virus) is demonstrated on visual labeling of cell membrane antigens. The hybrid antibody technique is preferably assumed to allow a multiple labeling of distinct antigens, scanning electron microscopical examinations and quantitative investigations in the field of immuno electron microscopy.

[Preparation of hybrid antibodies with mouse IgG and ferritin specifities for the immune electron microscopic detection of cell membrane antigens]. / Herstellung von Hybridantikörpern gegen Maus-IgG und gegen Ferritin zur immunelektronenmikroskopischen Darstellung von Zellmembranantigenen.

Use of hybrid antibodies with one specifity for mouse-IgG and the other one for ferritin is particularly suitable for immune electron microscopical detection of cell surface antigens. Preparation of antibodies of such kind is described, whereby the method introduced by HMMERLING et al. is varied within some steps of preparation. These variations are discussed here. Activity of produced hybrid antibodies is demonstrated by labeling the THY 1.1. antigen on the cell surface of the thymocytes of the mouse. The advantages of utilizing the hybrid antibodies in comparison with known immune electron microscopical techniques are an excellent location of the antigens, the possibility of using distinct particles for labeling, the application of a multiple labeling, and the fact that investigations by both the transmission and the scanning electron microscope can be carried out by means of the same preparation of hybrid antibodies.

[A case of central core disease. Electronmicroscopic studies (author's transl)]. / Beitrag zur Central Core Disease. 2 Mitteilung: Elektronenoptische Untersuchungen

A biopsy taken from the right m. gastrocnemius of a sporadic case of central core disease has been investigated by electronmicroscopy. Both longitudinal and cross sections show cores, which are partially in the centre and partially near the sarcolemma of the muscle fibres. These cores are more or less sharply delimited from the intact areas of the muscle fibres. A zone showing a severe destruction of the regularly fibrillar structure is observed in the centre of each core. This destruction zone is surrounded by a transition zone, in which a partial lesion of the fibrillar structure is found whereas the sarcomeric segmentation is preserved. The width of the interfibrillar sarcoplasm is reduced, frequently, however, mitochondria containing cristae of normal structure are observed. The cores are foundlimited by target fibres. Pathogenetically, the damages of z-lines are assumed to be dominant in the formation of the cores.

[Electron microscopical findings in the sural nerve of twins with metachromatic leukodystrophy. An attempt to classify the findings according to distinctive phases of the disease (author's transl)]. / Elektronenmikroskopische Befunde am Nervus suralis von Zwillingskindern mit metachromatischer Leukodystrophie. Versuch einer Zuordnung zu unterschiedlichen Erkrankungsstadien

Sural nerve biopsies taken from a pair of twins with metachromatic leukodystrophy were investigated by electron microscopy. The morphological findings showed distinct alterations. In the first twin the particularity was a large number of granules containing sulfatides. In the second twin, however, the main finding was a damage of myelin sheaths evidently caused by an excessive and morphologically as well as chemically defective myelin formation. According to the grading given by Ulrich the first case corresponds to the stadium one, in which a storage of sulfatides dominates, whereas case two showing a severe damage of myelin sheaths is characteristic of the second stadium of metachromatic leukodystrophy.