[H3-dopamine and H3-quinuclidinylbenzilate binding by autonomic ganglia tissue in rats of various age]. / Sviazyvanie 3H-dofamina i 3H-khinuklidinilbenzilata tkan'iu vegetativnykh gangliev krys raznogo vozrasta.

Binding of tritiated ligands of muscarinic and dopamine receptors was analysed in rats 1, 7, 14, 28, 60 days and 24-30 months old. The following ganglia were studied: the nodose ganglion, the lumbar ganglia of sympathetic chain, the main pelvic ganglion in male rats and the paracervical ganglion in female rats. The same level was found for binding of each of ligands for all investigated ganglia. Parameters of postnatal dynamics of development M-cholino- and dopamine reception systems prove to be quite similar, but not identical. Both of the systems reach matured level during first 2 postnatal weeks. In comparison with quinuclidinylbenzilate binding failure of dopamine binding in aged rats is to be more pronounced.

[Age-related changes in the amount and intensity of the fluorescence of small, intensely fluorescing cells in the autonomic ganglia of rats]. / Vozrastnye izmeneniia kolichesva i intensivnosti fliuorestsentsii melkikh, intensivno fliuorestsiruiushchikh kletok vegetativnykh gangliev krysy.

The number and intensity of fluorescence of small, intensely fluorescent cells were measured on serial slices of main pelvic (MPG) ganglion and lumbar ganglia of sympathetic trunk (LG), treated by modified Falck method, on days 1, 7, 14, 28, and 26-30 months of age. The content of paraform-induced fluorescence increased with age of two weeks and later in SIF cells of MPG and LG. The number of SIF cells in LG decreased with age, while that of MPG increased. The growth of a number of SIF cells in MPG was detected in large clusters.

[Changes in the transcription capacity of the chromatin of nerve and liver cells exposed to morphine and opioids]. / Izmenenie transkriptsionnoi sposobnosti khromatina nervnykh i pechenochnykh kletok pri deistvii na nikh morfina i nekotorykh opioidov.

Morphine stimulates in vitro 3H-UTP incorporation into the nuclei of neurons of the superior cervical ganglion and into the hepatocyte nuclei of some rodents. This effect is dose-dependent and partially inhibited by naloxone. A synthetic analog of enkephalins, D-Ala2-Gly-ol5-enkephalin (DAGO), and beta-endorphin stimulate the incorporation of RNA precursors into pre-rRNA. Possible mechanisms of morphine action are discussed.

[Changes in the properties of chromatin during nerve cell acquisition of definitive morphologic features]. / Izmeneniia svoistv khromatina v protsessee priobreteniia nervynymi kletkami definitivnykh morfologicheskikh priznakov.

The morphological maturation of neurocytes of the cranial cervical ganglion during the 1st month of life was accompanied in rats by changes in the state of DNA-histone chromatin complex and rise of stainability of non-histone nuclear proteins by fast green FCF at pH 2.6. This coincided in time with the increase of transcription level as well as with changes in the dynamics of RNA-bound nuclear label during the first hours after (3)H-uridine incorporation. These peculiarities reflect the process of chromatin rearrangement during the maturation of neurocytes.