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Appl Opt ; 50(6): 788-96, 2011 Feb 20.
Article in English | MEDLINE | ID: mdl-21343956

ABSTRACT

We have evaluated the influence of growth media and washing on the laser-induced fluorescence spectra of bacteria. Three different bacterial simulants were cultured in three types of growth media. Three kinds of samples were generated from each culture: the culture itself, the growth medium alone, and a triple-washed sample. The materials were injected as aerosols in a lab-sized lidar aerosol chamber to obtain their spectra. Using two different analysis approaches, signature variations were observed between the three kinds of samples for most combinations of growth media/bacteria. This study concludes that the culture media used influences the spectral signatures.


Subject(s)
Aerosols/chemistry , Culture Media, Conditioned/chemistry , Spectrometry, Fluorescence/methods , Bacillus/chemistry , Bacillus/metabolism , Biological Warfare Agents , Erwinia/chemistry , Erwinia/metabolism , Pattern Recognition, Automated/methods , Principal Component Analysis
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