ABSTRACT
The authors report about one case of acute abdominal pain syndrome, the etiology of which proved to be a primary torsion of the greater omentum. On this basis, they review the international literature and set forth etiopathogenetic hypotheses.
Subject(s)
Omentum/surgery , Peritoneal Diseases/surgery , Abdomen, Acute/etiology , Abdomen, Acute/surgery , Adult , Humans , Male , Omentum/physiopathology , Peritoneal Diseases/etiology , Time Factors , Torsion AbnormalityABSTRACT
The human erythrocyte glucose transporter is a fully integrated membrane glycoprotein having only one N-linked carbohydrate chain on the extracellular part of the molecule. Several authors have suggested the involvement of the carbohydrate moiety in glucose transport, but not definitive results have been published to date. Using transport glycoproteins reconstituted in proteoliposomes, kinetic studies of zero-trans influx were performed before and after N-glycanase treatment of the proteoliposomes: this enzymatic treatment results in a 50% decrease of the Vmax. The orientation of transport glycoproteins in the lipid bilayer of liposomes was investigated and it appears that about half of the reconstituted transporter molecules are oriented properly. Finally, it could be concluded that the release of the carbohydrate moiety from the transport glycoproteins leads to the loss of their transport activity.
Subject(s)
Blood Glucose/metabolism , Erythrocytes/metabolism , Monosaccharide Transport Proteins/blood , Amidohydrolases/metabolism , Biological Transport , Glycosylation , Humans , Kinetics , Lipid Bilayers/metabolism , Liposomes/metabolism , Monosaccharide Transport Proteins/chemistry , Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase , Structure-Activity RelationshipABSTRACT
ATP entrapped into liposomes was administered intracarotidally to rats submitted to brain ischemics episodes by clamping of the carotid arteries and lowering of the systemic blood pressure. It was observed that when entrapped into liposomes, ATP greatly increased the number of ischemic episodes tolerated before brain electrical silence and death appeared. These results added to very similar previous data obtained by i.c.v. treatment excluding the prominent role of cardiovascular effects, could open new possibilities in brain antihypoxic protection. Here and now it cannot be stated if ATP provides direct energetic supply.
Subject(s)
Adenosine Triphosphate/pharmacology , Brain Ischemia/drug therapy , Adenosine Triphosphate/administration & dosage , Adenosine Triphosphate/therapeutic use , Animals , Blood Pressure/drug effects , Carotid Arteries , Electroencephalography , Injections, Intra-Arterial , Liposomes , Male , Rats , Rats, Inbred StrainsABSTRACT
Liposomally entrapped adenosine triphosphate (ATP) was administered intracerebroventricularly and intracarotidally to rats subjected to brain ischaemic episodes by clamping of the carotid arteries and lowering of the systemic blood pressure. It was observed that, when entrapped in liposomes, ATP greatly increased the number of ischaemic episodes before brain electrical silence and death. The results open new perspectives in brain ATP supply, which will potentially be useful in human resuscitation from deep brain hypoergic states.
Subject(s)
Adenosine Triphosphate/therapeutic use , Brain Ischemia/drug therapy , Liposomes/administration & dosage , Adenosine Triphosphate/administration & dosage , Animals , Blood-Brain Barrier , Injections, Intra-Arterial , Injections, Intraventricular , Male , Rats , Rats, Inbred StrainsABSTRACT
ATP was entrapped inside negatively charged liposomes composed of sulfatide, in order to improve its penetration into the brain and to reduce its degradation into other tissues. These liposomes were prepared according to an original method allowing a satisfying stability of the formulation. Liposomally entrapped ATP was administered intracerebroventricularly to rats submitted to brain ischemic episodes by both carotid artery clamping and systemic blood pressure lowering (during 3 minutes every 15 minutes). Such treatment importantly increases the number of ischemic episodes before brain silence appeared. So, this paper allows new perspectives in the administration of drugs into the brain.
Subject(s)
Adenosine Triphosphate/administration & dosage , Brain Ischemia/drug therapy , Liposomes/administration & dosage , Adenosine Triphosphate/therapeutic use , Animals , Biological Availability , Injections, Intraventricular , Male , Rats , Rats, Inbred Strains , SulfoglycosphingolipidsABSTRACT
Two novel complexes of Pd(II) involving vitamin B6 compounds have been synthesized. They are compatible with the compositions Pd(P.H.)2 C2(P = pyridoxol) and Pd(PL.H)2 C2(PL = pyridoxal). The complexes inhibited the growth as well as the biosynthesis of RNA, DNA, and protein of E. coli B-766. Photoacoustic spectral (PAS) measurements showed that the complexes bound to DNA of the bacteria and were present only in the kidney of treated mice. The complexes inhibited the incorporation of 3H-thymidine as well as 14C-leucine in the DNA and protein, respectively, of liver cell cultures (BL8L). The inhibition of cell division of Walker-S-cells and human lymphocytes by the complexes was highly significant.
Subject(s)
Cell Division/drug effects , Lead/pharmacology , Pyridoxine/pharmacology , Animals , DNA, Bacterial/isolation & purification , Escherichia coli/cytology , Humans , Lead/metabolism , Mice , Pyridoxine/metabolism , Spectrophotometry, InfraredABSTRACT
The authors report two cases of torsion of Riedel's lobe. The first case resembled acute cholecystitis. Resection was followed by a cure. The second case presented in the form of a complicated abdomino-pelvic tumor. Its removal did not prevent a fatal issue due to fibrinolysis probably related to advanced ischemic infarction of Riedel's lobe of the liver. It seems that these complications are exceptional.
