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1.
J Biomed Opt ; 19(4): 045002, 2014 Apr.
Article in English | MEDLINE | ID: mdl-24723112

ABSTRACT

Detecting and concentrating cancer cells in peripheral blood is of great importance for cancer diagnosis and prognosis. Optically induced dielectrophoresis (ODEP) can achieve high resolution and low optical intensities, and the electrode pattern can be dynamically changed by varied light patterns. By changing the projected light pattern, it is demonstrated to separate high-purity gastric cancer cell lines. Traditionally, the purity of cancer cell isolation by negative selection is 0.9% to 10%; by positive selection it is 50% to 62%. An ODEP technology is proposed to enhance the purity of cancer cell isolation to about 77%.


Subject(s)
Cell Separation/methods , Electrophoresis/methods , Microfluidic Analytical Techniques/instrumentation , Optical Imaging/methods , Cell Line, Tumor , Cell Separation/instrumentation , Electrophoresis/instrumentation , Equipment Design , Humans , MCF-7 Cells , Neoplasms , Optical Imaging/instrumentation
2.
J Proteome Res ; 12(8): 3573-85, 2013 Aug 02.
Article in English | MEDLINE | ID: mdl-23782096

ABSTRACT

Malignant tumors are relatively resistant to treatment due to their heterogeneous nature, drug resistance, and tendency for metastasis. Recent studies suggest that a subpopulation of cancer cells is responsible for the malignant outcomes. These cells are considered as cancer stem cells (CSC). Although a number of molecules have been identified in different cancer cells as markers for cancer stem cells, no promising markers are currently available for hepatocellular carcinoma cells. In this study, two clones of Hep3B cell lines were functionally characterized as control or CSC-like cells, based on properties including spheroid formation, drug resistance, and tumor initiation. Furthermore, their protein expression profiles were investigated by isobaric tags for relative and absolute quantitation (iTRAQ), and a total of 1,127 proteins were identified and quantified from the combined fractions; 50 proteins exhibited at least 2-fold differences between these two clones. These 50 proteins were analyzed by GeneGo and were found to be associated with liver neoplasms, hepatocellular carcinoma (HCC), and liver diseases. They were also components of metabolic pathways, immune responses, and cytoskeleton remodeling. Among these proteins, the expressions of S100P, S100A14, and vimentin were verified in several HCC cell lines, and their expressions were correlated with tumorigenicity in HCC cell lines. The functional significance of vimentin and S100A14 were also investigated and verified.


Subject(s)
Biomarkers, Tumor/genetics , Carcinoma, Hepatocellular/genetics , Cell Transformation, Neoplastic/genetics , Liver Neoplasms/genetics , Neoplastic Stem Cells/metabolism , Proteome/genetics , Biomarkers, Tumor/metabolism , Carcinoma, Hepatocellular/metabolism , Carcinoma, Hepatocellular/pathology , Cell Line, Tumor , Cell Transformation, Neoplastic/metabolism , Cell Transformation, Neoplastic/pathology , Drug Resistance, Neoplasm/genetics , Gene Expression , Gene Expression Profiling , Humans , Liver Neoplasms/metabolism , Liver Neoplasms/pathology , Mass Spectrometry , Molecular Sequence Annotation , Neoplastic Stem Cells/pathology , Proteome/metabolism , S100 Proteins/genetics , S100 Proteins/metabolism , Staining and Labeling/methods , Vimentin/genetics , Vimentin/metabolism
3.
Article in English | MEDLINE | ID: mdl-23304212

ABSTRACT

Chlorella sorokiniana (CS) is a unicellular green alga. The extracts of Chlorella have been used as treatments for relieving hypertension and modulating immune response. The detailed mechanisms are not clear yet. In this study, we sought to study the molecular mechanisms for the polysaccharide fraction of CS-induced immune response. We pulsed dendritic cells (DCs) with CS and found that CS could maturate DCs. CS-maturated DC could activate naïve T cells and stimulate T-cell proliferation and IFN-γ secretion. Furthermore, CS activated PI3K and MAPKs signaling pathways in DCs by interacting with TLR4 receptor. These CS-activated signaling pathways could further activate NF-κB and induce IL-12 production in DCs. This study provides molecular mechanisms for CS-induced DCs activation and immune response.

4.
Bioorg Med Chem Lett ; 20(20): 6129-32, 2010 Oct 15.
Article in English | MEDLINE | ID: mdl-20833039

ABSTRACT

A series of azulene-based derivatives were synthesized as potent inhibitors for receptor tyrosine kinases such as FMS-like tyrosine kinase 3 (FLT-3). Systematic side chain modification of prototype 1a was carried out through SAR studies. Analogue 22 was identified from this series and found to be one of the most potent FLT-3 inhibitors, with good pharmaceutical properties, superior efficacy, and tolerability in a tumor xenograft model.


Subject(s)
Antineoplastic Agents/chemistry , Antineoplastic Agents/therapeutic use , Azulenes/chemistry , Azulenes/therapeutic use , Leukemia, Myeloid, Acute/drug therapy , Receptor Protein-Tyrosine Kinases/antagonists & inhibitors , Animals , Antineoplastic Agents/blood , Antineoplastic Agents/pharmacology , Azulenes/blood , Azulenes/pharmacology , Cell Line, Tumor , Cell Proliferation , Humans , Mice , Mice, Inbred BALB C , Mice, Nude , Rats , Receptor Protein-Tyrosine Kinases/metabolism , fms-Like Tyrosine Kinase 3/antagonists & inhibitors
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