Enhanced nitrogen removal performance of nitrogen-rich saline wastewater by marine anammox bacteria: Based on different influent loading strengths.

In an anaerobic sequential batch reactor (SBR), marine anammox bacteria (MAB) were able to enhance microbial activity in nitrogen-rich saline wastewater and it was significantly affected by influent substrate composition and loading strength. This study therefore enhanced nitrogen removal efficiency by adjusting the influent nitrogen loading strength of MAB-inoculated anaerobic SBRs and assessed the correlation with the bacterial community. The results displayed that the system obtained optimal nitrogen removal efficiency (TN = 83.52%, NH4-N = 90.14%, and NO2-N = 83.57%) as the strength of influent nitrogen loading was increased to 201.35 mg L-1 for NH4-N and 266.42 mg L-1 for NO2-N. Moreover, the increase in the strength of influent nitrogen loading also enhanced the anammox 16S rRNA abundance (4.09 × 108 copies g-1) and ladderanes content (22.49 ng g-1dw). Analysis of 15N isotope further illustrated that all systems were dominated by anammox (average ra = 95.22%). In conclusion, these findings provide scientific guidance for the management of eutrophic seawater and contribute to the realization of industrial applications for the treatment of nitrogen-rich saline wastewater.

Nitrogen loss from the coastal shelf of the East China Sea: Implications of the organic matter.

Organic matter is a critical factor which regulates nitrogen loss pathways of denitrification and anammox for microbes in marine ecosystems. However, only a little attention has been paid to contrasting studies on denitrification and anammox in sandy and muddy sediments, especially in the coastal continental shelf dominated by sandy sediments. This study determined the bulk properties and associated microbial nitrogen transformation processes of surface sediments in the East China Sea coastal shelf, with the aim of gaining insight into the interaction of nitrogen loss with organic matter at the molecular level. The results illustrate that nitrogen loss dominates in organic-rich muddy sediments, and its denitrification rate (14.39 nmol N g-1 h-1) and anammox rate (2.73 nmol N g-1 h-1) are greater than those of sandy sediments (denitrification rate = 5.55 nmol N g-1 h-1, anammox rate = 1.57 nmol N g-1 h-1). Furthermore, determination of the mean summed ladderanes shows higher anammox generated in the muddy sediments with a value of 167.78 ng g-1dw. Quantitative analysis demonstrated that organic-rich muddy sediments enhanced the copy number of the denitrifying functional gene nosZ and anammox functional gene hzsB. We inferred that the greater rate of nitrogen loss in muddy sediments was due to the coupling relationship between anammox and denitrification. Overall, the community distribution and abundance of denitrifying bacteria and anammox bacteria changed intricately under the influence of organic matter. Moreover, this study further improves the understanding of nitrogen loss pathways and mechanistic factors from sediments.

Enhanced nitrogen removal in filled-and-drained vertical flow constructed wetlands: microbial responses to aeration mode and carbon source.

For the purpose of enhancing the removal rate of nitrogen (N) and organic matters, intermittent aeration and carbon source were used in filled-and-drained vertical flow constructed wetlands (VFCWs). The results showed that the best removal of COD (74.16%), NH4+-N (93.56%), TN (86.88%), and NO3--N (79.65%) was achieved in VFCW1 (aerated with carbon source system). Illumina MiSeq300 high-throughput sequencing showed that carbon source aerated system increases the diversity and richness of the microbial community. The copy numbers of nitrification functional genes (nxrA, amoA), denitrification functional genes (nirS, nirK, nosZ), and anammox functional gene (anammox 16S rRNA) displayed various changes when applied different aeration modes and additional carbon source to each system. An increase of the DO concentration and carbon source facilitated the absolute abundance of microbial nitrification and denitrification functional genes, respectively. All in all, these results demonstrate that carbon source combined with intermittent aeration is valid to improve the pollutant treatment performance in these systems.

The simultaneous antibiotics and nitrogen removal in vertical flow constructed wetlands: Effects of substrates and responses of microbial functions.

A vertical flow constructed wetland (VFCW) packed with the different substrates was designed to remediate the antibiotics in the wastewater. Zeolite (CW-Z) paralleled with Manganese (Mn) ore (CW-M) and biochar (CW-C) were used to enhance the synchronous removal of ciprofloxacin hydrochloride (CIPH), sulfamethazine (SMZ) and nitrogen (N) from the wastewater. The result indicated that CW-M had a significant potential to remove CIPH (93%), SMZ (69%), TN (71%), NH4+-N (94%) and NO3--N (94%) across all the treatments. The abundance of amoA, nirK and nirS genes are dramatically higher in CW-M and CW-C, while CW-C inhibited the production of quinolone resistance genes. Results showed that different substrates could affect the microbial diversity and structure. The addition of Mn ore to the water led to an improved abundance of nitrogen-related phyla. Overall, Mn ore has a considerable potential to simultaneously remove antibiotics and N in VFCWs.

Enhanced optimal removal of nitrogen and organics from intermittently aerated vertical flow constructed wetlands: Relative COD/N ratios and microbial responses.

Carbon source and dissolved oxygen are the critical factors which sustain the stable redox environment for the microbes to implement the removal of nitrogen and organics in vertical flow constructed wetlands (VFCWs). The effect mechanisms of the COD/N ratios in intermittently aerated VFCWs are needed to be investigated in order to increase the synchronous removal efficiency of pollutants. In this study, the combined effects of COD/N ratios (3, 6, 12) and intermittent aeration in VFCWs on pollutant removal, microbial communities and related function genes were studied. The results showed the increase of COD/N ratios from 3 to 12 enhanced the removal efficiency of TN, NO3--N and COD. The removals of NH4+-N decreased as the COD/N ratio increased. The optimal removals of TN (87.65%), NH4+-N (93.20%), NO3--N (80.80%) and COD (73.93%) were obtained in VFCW2 (COD/N ratios was 6). Illumina Miseq High-throughput sequencing analysis showed that high COD/N ratios increased the richness and diversity of microbial communities. The absolute abundance of nirK, nosZ, nirS, amoA, nxrA, and anammox bacterial 16S rRNA presented various changes under the different ratios of COD/N. The increase of COD/N ratios enhanced the copy numbers of nirS, nirK and nosZ, which participate in denitrification process. High COD/N ratios (6 and 12) were in favor of Actinobacteria, Firmicutes and Chloroflexi, which mainly play important roles in the process of denitrification. This paper implies that the combination of carbon source and aeration is necessary to sustain high microbial activities during pollutant removal in VFCWs.

Combing δ15N and δ18O to identify the distribution and the potential sources of nitrate in human-impacted watersheds, Shandong, China.

Identifying the anthropogenic and natural sources of nitrate emissions contributing to surface water continues to be an enormous challenge. It is necessary to control the water quality in the watershed impacted by human disturbance. In this study, water chemical parameters including nitrate (NO3 -) concentrations, δ15N-NO3 -, δ18O-NO3 -, and δ18O-H2O were analyzed to investigate the contamination and sources of NO3 - in two watershed rivers (Jinyun, JYN and Jinyang, JYA), Jinan, Shandong, China. Results indicated NO3 - concentrations in the JYN were significantly higher than those in the JYA (P < 0.05), probably because of high N input of the extensive farmlands or orchards in the drainage basin. δ15N-NO3 - and δ18O-NO3 -, associated with Cl-, indicated that nitrate-nitrogen (NO3 --N) was not derived from atmospheric deposition but came principally from manure/sewage and soil organic matter in these two watersheds. The microbial nitrification took place in the nitrate of manure/sewage and soil nitrate. The combination of NO3 - concentration and nitrogen and oxygen isotope suggested that NO3 - had undergone microbial denitrification after entering the rivers. Furthermore, NO3 - concentrations had significant temporal and spatial variation highlighting differential sources and fates. These results expand our understanding of mechanisms driving NO3 - retention and transport and provide strategies in managing NO3 - contamination in different land use watersheds around the world.