Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 1 de 1
Filter
Add more filters










Database
Language
Publication year range
1.
Biochem Biophys Res Commun ; 430(1): 370-4, 2013 Jan 04.
Article in English | MEDLINE | ID: mdl-23149414

ABSTRACT

While Mdm2 is an important negative regulator of the p53 tumor suppressor, it also possesses p53-independent functions in cellular differentiation processes. Mdm2 expression is alternatively regulated by two P1 and P2 promoters. In this study we show that the P2-intiated transcription of Mdm2 gene is activated by 1,25-dihydroxy vitamin D3 in MC3T3 cells. By using P1 and P2-specific reporters, we demonstrate that only the P2-promoter responds to vitamin D treatment. We have further identified a potential vitamin D receptor responsive element proximal to the two p53 response elements within the Mdm2 P2 promoter. Using cell lines that are p53-temperature sensitive and p53-null, we show requirement of p53 for VDR-mediated up regulation of Mdm2 expression. Our results indicate that 1,25-dihydroxy vitamin D3 and its receptor have a role in the regulation of P2-initiated Mdm2 gene expression in a p53-dependent way.


Subject(s)
Calcitriol/metabolism , Gene Expression Regulation , Osteoblasts/metabolism , Proto-Oncogene Proteins c-mdm2/genetics , Vitamin D Response Element/genetics , Animals , Calcitriol/pharmacology , Cell Line , Mice , Osteoblasts/drug effects , Receptors, Calcitriol/metabolism , Tumor Suppressor Protein p53/metabolism , Up-Regulation , Vitamin D Response Element/drug effects
SELECTION OF CITATIONS
SEARCH DETAIL
...