Experimental population design for estimation of dominant molecular marker effect on egg-production traits.

A potential limitation of the use of a dominant molecular marker system such as DNA fingerprinting (DFP) is the inability to distinguish homozygous from heterozygous allele state in an individual, and a resulting inaccuracy in estimating effects of the marker alleles. The objective of this study was to accurately estimate the effect of DFP markers on egg-production traits. A BC1 population was produced from two distinct layer lines. Four DFP bands, each originating predominantly in one of the two parental lines, were evaluated for linkage with egg-production quantitative trait loci in the BC1 population. The egg-production traits consisted of eight early period and seven late period measurements. Eight marker-trait linkages were identified out of 60 total statistical tests. By utilizing information on frequency of DFP bands in two parental lines, selecting F1 sires with DFP bands present, and backcrossing to the line lacking these bands, the population design allowed definitive identification of the DFP zygosity in the BC1 resource population hens. In this manner, accurate estimates of marker allele effects on egg-production traits were obtained from the dominant marker system of DNA fingerprinting.

Genetic diversity at the major histocompatibility complex (B) and microsatellite loci in three commercial broiler pure lines.

Genetic diversity at the MHC and non-MHC loci was investigated in three commercial broiler chicken pure lines. The MHC class II and IV loci were evaluated in Southern hybridizations and molecular genotypes based on RFLP were interpreted from pedigreed families. Four MHC class II and eight class IV genotypes were identified in the broiler lines, and their frequencies differed among the lines. Line-specific MHC genotypes were identified. The observed heterozygosities (59 to 67%) suggest that the MHC loci are highly polymorphic in the broiler lines. At least 9% of the genetic variation at the MHC was due to line differences; the remainder reflected individual variations. To characterize non-MHC genes, 41 microsatellite loci located throughout the chicken genome were evaluated in the broiler lines. Genetic variation was also observed at the microsatellite loci for the broiler lines; the number of alleles at a single locus ranged from one to eight, and the average number of alleles per locus was 3.5, 2.8, and 3.1 for each of the lines, respectively. The observed heterozygosities for microsatellite loci ranged between 0 and 89% in the lines. Based on the fixation index (Fst), about 19% of the genetic variation at microsatellite loci was attributed to broiler line differences. Deviations from Hardy-Weinberg equilibrium were detected at both MHC and non-MHC loci. Possible explanations for these deviations include genetic selection by the primary broiler breeder or the presence of null alleles that were not identified by the typing procedures described in this report. This study contributes to our knowledge on the molecular characteristics and genetic structure of a commercial broiler chicken population. Analysis of MHC and non-MHC loci suggests that there is still sufficient genetic diversity in the broiler lines to continue the progress toward improved broiler chicken production.

Salmonella enterica serovar enteritidis burden in broiler breeder chicks genetically associated with vaccine antibody response.

The relationship between antibody response to Salmonella enteritidis vaccine and internal organ burden of S. enteritidis is not fully understood. The genetic relationship, therefore, between postchallenge S. enteritidis burden and antibody response to S. enteritidis vaccine was determined in broiler breeder chicks. Sibling chicks from a broiler breeder male line were either inoculated with a pathogenic S. enteritidis or vaccinated with a commercial S. enteritidis vaccine. Spleen, liver, cecal wall, and cecal content samples from S. enteritidis-challenged chicks (n = 120) were cultured for enumeration of bacteria. Unchallenged chicks (n = 314) were vaccinated at 11 days of age, and serum samples were taken at 10 days postvaccination. Antibody response to vaccination and number of S. enteritidis in cecal content cultures were negatively correlated (-0.772), demonstrating that genetic potential for greater antibody response to S. enteritidis vaccine is associated with lesser S. enteritidis bacterial burden in cecal content of broiler breeder chicks. The findings suggest that genetic selection for vaccine antibody responsiveness can lower bacterial burden in the gut lumenal content and, thus, potentially reduce contamination of poultry products at processing.

Behaviour of buildings to withstand cyclonic winds : An experience of Gujarat and Andhra Pradesh

India due to its geographical location, has 5,700 kms long coastal line in the southern hemisphere of the earth. It faces four to five cyclones every year. Out of which one or two are very severe. Cyclones impact 50 per cent of population of the country. Due to complexities of cyclonic winds and their dynamic nature, behavior of buildings and their response can not be predicted on theoretical analysis only. In fact, this needs to be studied historically to improve design of buildings. SERC, Chennai has pioneered in the same and have arrived at specific guidelines and findings for structural improvements in residential as well as other buildings preventing devastating impacts. Some extracts of such findings and guidelines have been brought out on this paper. (AU)

Rfp-Y region polymorphism and Marek's disease resistance in multitrait immunocompetence-selected chicken lines.

Although the influence of the chicken classical MHC in resistance to many diseases is well established, the role of the recently identified, genetically independent, MHC-like region known as Rfp-Y is unclear. The objectives of this study were to analyze the frequencies of DNA polymorphisms of the Rfp-Y region in White Leghorn lines, which were divergently selected in replicate for multitrait immunocompetence, and to determine the association of these polymorphisms with Marek's disease (MD) resistance. Chicks, either with or without herpes virus of turkey (HVT) vaccination, were challenged with 500 ffu of a very virulent Marek's disease virus (Md5) at 2 d of age. The MD-related data were collected for 10 wk. PvuII-digested genomic DNA was hybridized with an Rfp-Y region-specific probe, 18.1. Three Rfp-Y polymorphisms were observed. The frequency of one Rfp-Y polymorphism was significantly different between divergently selected multitrait immunocompetence lines in one replicate only; therefore, the impact of multitrait immunocompetence selection on Rfp-Y polymorphisms is inconclusive. The PvuII defined Rfp-Y region polymorphisms had no association with either innate or vaccine-induced MD resistance to Md5 virus challenge.

Major histocompatibility complex class II DNA polymorphisms in chicken strains selected for Marek's disease resistance and egg production or for egg production alone.

The objective of this study was to investigate frequencies of major histocompatibility complex (MHC) class II restriction fragments in two groups of White Leghorn strains. Each group consisted of an unselected control, a strain selected for egg production traits, and a strain selected for egg production traits and Marek's disease (MD) resistance. PvuII-digested genomic DNA was hybridized with a chicken genomic MHC class II probe. The MHC class II DNA fragment frequencies in the selected strains differed from those in the related unselected control and in the strain selected using the same criteria from a different base population. Based on the sizes of the breeding populations, particularly those in the control strain and in the strain selected for egg production, it was considered unlikely that the observed changes of the MHC class II fragment frequencies were due to random genetic drift. The data suggested that some MHC class II bands are associated with production traits or with MD resistance, and that these associations tend to be unique to each genetic background. Hence, MHC class II genes are likely candidates for the investigation of quantitative trait loci in egg production and disease resistance traits such as those for which the studied strains were selected.

Genetic markers linked to quantitative traits in poultry.

This study utilized DNA fingerprints and crosses of two genetically distinct lines of layer-type chickens to identify genetic markers linked to quantitative trait loci (QTL). In phase I, backcross (BC1) hens were separately ranked for each of eight traits and then blood pools were produced in groups along each phenotypic distribution. The DNA was isolated from the blood pools and used in a gradient analysis to screen for DNA fingerprint bands that exhibited intensity gradients associated with the phenotypic traits. To identify linkage of bands with QTL and to estimate band effects, F2 progeny were produced in phase II from the phase I BC1 population. A single-trait animal model was used for analysis of associations of all individual DNA fingerprint bands of sires and their progeny phenotypic performance. Twenty fingerprint bands, only two of which had shown trait-associated gradients in phase I, were identified by the animal model analysis of the progeny test as QTL linked (P < or = 0.05) to specific traits of growth, reproduction and egg quality. These 20 bands warrant further study as potentially valuable molecular markers for QTL.

Sheep red blood cell and Brucella abortus antibody responses in chickens selected for multitrait immunocompetence.

Chickens from replicated lines divergently selected for multitrait immunocompetence were tested for their antibody responses to sheep red blood cells and Brucella abortus antigen. All birds were injected with sheep red blood cells and B. abortus antigens at 4 and 9 wk of age, and blood samples were taken 6, 8, and 10 d postimmunization. Antibody titers were determined by using agglutination assays. For sheep red blood cell responses, the most marked difference was between the high and low lines postsecondary immunization. The high line titer was still rising at 10 d, whereas the low line had reached its peak at 8 d and was decreasing. This may be a result of the selection process in which the high line is selected for a high, persistent response to two T cell-dependent antigens at 3 wk postimmunization. For B. abortus responses, the only differences were between high and low lines postsecondary immunization. The high lines were always higher than the low lines, with differences getting smaller as time postimmunization increased. In summary, selection for multitrait immunocompetence resulted in changes in antibody response to unrelated antigens.

Research note: influence of ahemeral light:dark cycles on egg traits in brown egg pullets.

Brown egg pullets (DeKalb Sex-Sal) were subjected to an ahemeral lighting program to determine their response in terms of egg traits. All birds were reared on a conventional lighting program of 10 h light (L):14 h dark (D) to 16 wk. At 16 wk, all birds received a schedule of 11L:13D followed by a weekly 1-h increase in photoperiod to 14L:10D at 19 wk. Control treatment (CON) birds were continued on this schedule. Ahemeral treatment (AHM) birds were given a 28-h schedule 14L:14D at 23 wk that was continued to 28 wk, then returned to a 24-h cycle of 14L:10D. Shell weight responded quickly to the ahemeral treatment and showed a significant (P less than .05) increase during the 2nd 28-h cycle. Shell thickness and total egg weight showed significant increases on the 3rd cycle; albumen weight showed an increase on Cycle 4, and yolk weight increased significantly only on the 8th cycle. When AHM treatment birds were returned to a conventional cycle (14L:10D) at 28 wk, the total weight remained significantly higher (P less than .05) as late as Cycle 6.

Effect of ahemeral light:dark cycles on egg production in early photostimulated brown-egg pullets.

A laying trial was conducted to determine the effect of an ahemeral lighting program on early photostimulated brown-egg pullets (DeKalb Sex-Sal). All birds received 24 h of light/day (24L:0D) to 3 days of age followed by 8 h of light (8L:16D) to 8 wk of age. From 8 to 16 wk the birds received 10 h of light daily. At 16 wk, the control group (CON) received 11 h of light and 13 h of darkness (11L:13D) followed by a weekly 1-h increase in photoperiod to 14L:10D at 19 wk, which was maintained for the duration of the trial (59 wk of age). Birds on the ahemeral (AHM) schedule were exposed to a 26-h schedule of 11L:15D at 16 wk with a weekly 1-h increase in the photoperiod to 14L:12D at 19 wk. The AHM schedule (14L:12D) was maintained from Weeks 19 to 30 at which time birds were returned to a 24-h cycle of 14L:10D and kept on this schedule for the remainder of the trial. Egg weight was significantly increased by the AHM treatment for 27 to 30 wk; however, there was no cumulative (Weeks 19 to 59) effect on egg weight. Percentages of eggs per hen per day (%HDP) were significantly reduced by the AHM treatment for the periods 23 to 26 wk and 31 to 34 wk. This reduction caused a significant cumulative effect on %HDP (68.9 for CON versus 66.2 for AHM).(ABSTRACT TRUNCATED AT 250 WORDS)