Socket preservation using a combination of propolis extract and bovine bone graft towards the expression of receptor activator of nuclear κB ligand and osteoprogerin.

AIM: This study was undertaken to comprehend the effect of a combination of bovine bone graft (BBG) and propolis extract on the receptor activator of nuclear κB ligand (RANKL) and osteoprotegerin (OPG) expressions in post-extraction tooth sockets.

Ethanol extract of propolis-bovine bone graft combination as a prospective candidate for socket preservation: Enhancing BMP7 and decreasing NFATc1.

OBJECTIVE: To analyse the expression of BMP7 in osteoblasts and NFATc1 in osteoclasts during the bone healing process in the extraction socket and the possible relationship between the expression of BMP7 and NFATc1. METHODOLOGY: This study represented a post-test only control group design consisting of four groups, namely; a control group (polyethylene glycol), an ethanol extract of propolis (EEP) group, a bovine bone graft (BBG) group, and a EEP-BBG group. 56 Cavia cobaya were split randomly into four groups. The mandibula left incisors of the subjects were extracted, treated with certain materials according to their group, and sutured. The expression of BMP7 and NFATc1 was observed on day 7 and day 14 by means of immunohistochemical staining. Statistical analysis was performed using a combination of one-way ANOVA, Games-Howell post-hoc, and Pearson tests. RESULTS: The propolis-BBG combination group showed the highest BMP7 expression, on both day 7 and day 14. With regard to NFATc1 expression, the combination group experienced the lowest expression on day 7 and day 14. The combination group showed significant differences in all expressions compared to the control group. The BMP7 and NFATc1 expressions showed a strong relationship (r = -0.598, r > 0.5). CONCLUSION: Propolis-BBG combination increases BMP7 expression and reduces NFATc1 expression in the extraction socket. This study confirmed a strong relationship between the expressions of BMP7 and NFATc1.

Lipopolysaccharides' Cytotoxicity on Human Umbilical Cord Mesenchymal Stem Cells

Abstract Objective: To show the cytotoxicity of Porphyromonas gingivalis lipopolysaccharide (LPS) on human umbilical cord mesenchymal stem cells (HUCMSCs) to better understand the characteristics for its application in regenerative procedures under periodontopathogen LPS influence. Material and Methods: Ultrapure Porphyromonas gingivalis LPS was used in this study. This research used a frozen stock HUCMSCs, previously confirmed by flow cytometry. The biological characteristics, such as cell morphology, proliferation, and protein expression, were screened. To check the cytotoxicity, HUCMSCs were cultured and divided into two groups, the control group and LPS group with various concentrations from 25 to 0.39 µg/mL. MTT assay was done and the cells were observed and counted. The significance level was set at 5%. Results: The percentage of living HUCMSCs on LPS group were not significantly different among concentrations (p>0.05) from 25 to 0.39 µg/mL, even though there were slight mean decrease between groups, but they were not significant. The duration of 24 hours of exposure of LPS does not significantly lower HUCMSCs viability. Conclusion: LPS does not affect the viability of HUCMSCs. The lower the concentration of LPS, the higher the viability of HUCMSCs.

Biocompatibility of Portunus Pelagicus Hydroxyapatite Graft on Human Gingival Fibroblast Cell Culture.

INTRODUCTION: Crab shell (Portunus pelagicus) has the potential to be a source of hydroxyapatite biomaterials that used as bone grafts. Before clinical application, crab shell graft should be tested for its biocompatibility in vitro on human gingival fibroblast. AIM: This study aimed to determine the biocompatibility of Portunus pelagicus hydroxyapatite graft on human gingival fibroblast cell culture. MATERIAL AND METHODS: Human gingival fibroblast cell cultures were divided into control group and treatment group with the addition of hydroxyapatite graft powder from Portunus pelagicus at a concentration of 100 ppm, 50 ppm, and 25 ppm. The synthesis process of hydroxyapatite was conducted by heating at 1000°C then characterizing the compound with SEM-EDX. All samples were incubated in α-MEM medium, then were given MTT material. The cultures on the plate were examined using ELISA reader. The results were analyzed using a Oneway Anova. RESULTS: The percentage of living cells throughout all treatment group shown results that exceeded the LD50 parameter. The highest percentage of living cells was at 25 ppm concentration group. CONCLUSION: The hydroxyapatite graft powder from crab shells is biocompatible with human gingival fibroblast cell culture.

Biocompatibility of Portunus Pelagicus Hydroxyapatite Graft on Human Gingival Fibroblast Cell Culture.

INTRODUCTION: Crab shell (Portunus pelagicus) has the potential to be a source of hydroxyapatite biomaterials that used as bone grafts. Before clinical application, crab shell graft should be tested for its biocompatibility in vitro on human gingival fibroblast. AIM: This study aimed to determine the biocompatibility of Portunus pelagicus hydroxyapatite graft on human gingival fibroblast cell culture. METHODS: Human gingival fibroblast cell cultures were divided into control group and treatment group with the addition of hydroxyapatite graft powder from Portunus pelagicus at a concentration of 100 ppm, 50 ppm, and 25 ppm. The synthesis process of hydroxyapatite was conducted by heating at 1000°C then characterizing the compound with SEM-EDX. All samples were incubated in α-MEM medium, then were given MTT material. The cultures on the plate were examined using ELISA reader. The results were analyzed using a Oneway Anova. RESULTS: The percentage of living cells throughout all treatment group shown results that exceeded the LD50 parameter. The highest percentage of living cells was at 25 ppm concentration group. CONCLUSION: The hydroxyapatite graft powder from crab shells is biocompatible with human gingival fibroblast cell culture.