ABSTRACT
Human endometrium from the secretory phase of the menstrual cycle was incubated with 3H- and 14C-labelled glucosamine and [3H]leucine. Incorporation into secreted extracellular glycoprotein and accumulation of the label into the microsomal fraction were measured. When oestradiol or progesterone were added to the medium, medroxyprogesterone acetate (MPA), ethynodiol diacetate and chlormadinone acetate reduced incorporation of glucosamine and MPA reduced incorporation of leucine into glycoprotein. MPA reduced the amount of glucosamine in the microsomal fraction and also had an effect on amino acid transport within the endometrial cells, as indicated by intracellular alpha-aminoisobutyric acid space measurements. These results and the ratios of 3H and 14C in the microsomal fraction and secreted protein suggest that MPA has a primary effect in decreasing amino sugar incorporation and a secondary effect in reducing amino acid incorporation into glycoprotein.
Subject(s)
Endometrium/metabolism , Glycoproteins/biosynthesis , Progesterone Congeners/pharmacology , Amino Acids/metabolism , Chlormadinone Acetate/pharmacology , Culture Techniques , Endometrium/drug effects , Estradiol/pharmacology , Female , Glucosamine/metabolism , Humans , Leucine/metabolism , Medroxyprogesterone/pharmacology , Microsomes/metabolism , Progesterone/pharmacology , Time FactorsSubject(s)
Endometrium/pathology , Infertility, Female/pathology , Biopsy , Carbohydrate Metabolism , Cell Nucleolus/metabolism , Embryo Implantation , Endometrium/physiology , Endometrium/ultrastructure , Estrogens/physiology , Female , Humans , Lysosomes/metabolism , Menstruation , Microscopy, Electron , Mitochondria/metabolism , Progesterone/metabolism , Protein Biosynthesis , RNA/biosynthesis , Uteroglobin/physiologyABSTRACT
PIP: Plasma levels of progesterone and estradiol-17beta were measured using radioimmunoassay methods in 52 blood samples obtained from 10 women aborting after intraamniotic instillation of prostaglandin F2alpha. Mean falls in plasma progesterone of 55.7% and in estradiol-17beta of 46.6% were noted. There was a highly significant correlation between falling concentrations of both steroids and the instillation-abortion interval (p 0.001).^ieng
